anti-Cow (Bovine) TACR1 antibody for Immunohistochemistry

Recommended TACR1 Antibody (supplied by: Log in to see )

Antigen
Tachykinin Receptor 1 (TACR1) Antibodies
  • NK1R
  • NKIR
  • SPR
  • TAC1R
  • Nk1r
  • Spr
  • Tac1r
  • nk1R
  • Tacr1
  • ASPR
  • tachykinin receptor 1
  • TACR1
  • Tacr1
Epitope
Extracellular Domain
23
19
15
14
11
8
7
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Human, Monkey
151
72
65
11
9
5
5
4
4
4
2
2
1
1
1
1
Host
Rabbit
137
16
9
Clonality
Polyclonal
Conjugate
This TACR1 antibody is un-conjugated
8
8
7
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
110
59
58
32
27
20
18
13
6
5
2
2
1
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Catalog No. ABIN1049097
$ 484.00
Plus shipping costs $45.00

General

Antigen Tachykinin Receptor 1 (TACR1) Antibodies
Epitope Extracellular Domain
(23), (19), (15), (14), (11), (8), (7), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Human, Monkey
(151), (72), (65), (11), (9), (5), (5), (4), (4), (4), (2), (2), (1), (1), (1), (1)
Host Rabbit
(137), (16), (9)
Clonality
Polyclonal
Conjugate This TACR1 antibody is un-conjugated
(8), (8), (7), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(110), (59), (58), (32), (27), (20), (18), (13), (6), (5), (2), (2), (1)
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Product Details anti-TACR1 Antibody

Target Details TACR1 Application Details Handling Images
Brand IHC-plus™
Specificity Human Neurokinin 1 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bovine, Dog (100%) Rabbit (94%) Elephant, Horse, Guinea pig (88%) Mouse, Rat, Hamster, Opossum (81%).
Purification Immunoaffinity purified
Immunogen Synthetic 16 amino acid peptide from 2nd extracellular domain of human Neurokinin 1 Receptor. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Bovine, Dog (100%), Rabbit (94%), Elephant, Horse, Guinea pig (88%), Mouse, Rat, Hamster, Opossum (81%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Target Details TACR1

Product Details anti-TACR1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name TACR1 / NK1R (TACR1 Antibody Abstract)
Background Name/Gene ID: TACR1
Subfamily: Tachykinin
Family: GPCR

Synonyms: TACR1, Neurokinin receptor 1, NK-1R, Nk1 tachykinin receptor, NK1R, TAC1R, Tachykinin 1 receptor, NKIR, Substance-P receptor, Neurokinin 1 receptor, NK-1 receptor, Tachykinin receptor 1
Gene ID 6869
Pathways Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration

Application Details

Product Details anti-TACR1 Antibody Target Details TACR1 Handling Images back to top
Application Notes Approved: IHC, IHC-P (2 - 4 μg/mL)
Comment

Target Species of Antibody: Human

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

Product Details anti-TACR1 Antibody Target Details TACR1 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months

Images

Product Details anti-TACR1 Antibody Target Details TACR1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Tachykinin Receptor 1 (TACR1) (Extracellular Domain) antibody (ABIN1049097) Anti-Neurokinin 1 Receptor antibody ABIN1049097 IHC staining of human brain, neurons...
Immunohistochemistry (IHC) image for anti-Tachykinin Receptor 1 (TACR1) (Extracellular Domain) antibody (ABIN1049097) Anti-TACR1 / NK1R antibody IHC of human Brain, Glioblastoma. Immunohistochemistry of ...