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|Antigen||Tachykinin Receptor 1 (TACR1) Antibodies|
|Reactivity||Guinea Pig, Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This TACR1 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
|31 references available|
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Product Details anti-TACR1 AntibodyTarget Details TACR1 Application Details Handling References for anti-TACR1 antibody (ABIN152451) Images
|Specificity||This antibody is specific to the NK-1 receptor. Staining of cells transfected with NK-1 was abolished by preabsorption of the antisera with NK-1. Specificity was also verified by the localization of immunohistochemical staining with NB300-101 in neurons in|
|Immunogen||A synthetic peptide corresponding to the C-terminus of rat Neurokinin 1 Receptor, conjugated to bovine thyroglobulin. [UniProt# P14600]|
|Plasmids, Primers & others|
Target Details TACR1Product Details anti-TACR1 Antibody Application Details Handling References for anti-TACR1 antibody (ABIN152451) Images back to top
|Alternative Name||NK1R (TACR1 Antibody Abstract)|
|Background||Gene Symbol: TACR1|
|Pathways||Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration|
Application DetailsProduct Details anti-TACR1 Antibody Target Details TACR1 Handling References for anti-TACR1 antibody (ABIN152451) Images back to top
|Application Notes||Western Blot 1:100-1:2000, Immunohistochemistry 1:1000, Immunocytochemistry/Immunofluorescence 1:50, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen 1:1000This Neurokinin 1 Receptor antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen sections and Western Blot. For ICC/IF staining, please see customer the review. Use in Immunohistochemistry on paraffin embedded sections was reported in scientific literature (PMID: 23792204).|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
Protocol specific for Neurokinin 1 Receptor Antibody Immunohistochemistry1) Perfuse rat through the ascending aorta with 500 mL. of 0.1 M phosphate buffered saline (PBS) (pH 7.4 at 4 degrees C), followed by 750 mL. of PBS containing 4 % formaldehyde and 12.5 % picric acid (pH 6.9, 4 degrees C). Tissue of interest is then dissected and post-fixed in PBS containing 4 % formaldehyde and 12.5 % picric acid (pH 6.9 at 4 degrees C) for at least 4 hours, followed by fixation in PBS containing 30 % sucrose (pH 7.4, 4 degrees C) for at least 24 hours. Until the tissue sinks.
2) Cut fixed sections at 60 um using a sliding microtome. 3) If using culture cells, remove media and perform 2-3 washed in PBS (pH 7.
) -use same protocol without agitation. 4) Place tissue section in microcentrifuge tubes containing 1 mL. Of PBS, pH 7.4 as they are being cut. Wash sections (in PBS) on an upright rotator for 10-15 minutes.
) Remove PBS and add 1 mL. of blocking solution (PBS + 1 % normal donkey serum (NDS) + 0.3 % triton X-100).
) Incubate tissue sections in blocking solution for 30 minutes at room temperature (RT) on rotator. 7) Remove blocking solution and add anti-neurokinin-1 receptor or anti-Neurokinin-3 receptor antibody +PBS + 1 % NDS + 0.3 % triton X-100 + NB 300-101/102 at 1:100~1:10008) Incubate overnight on rotator at RT.
) Remove primary antisera and perform 3 x 10 minute PBS washes (1ml volume) on rotator.
) Add 1 mL. of secondary antibody and incubate for 2 hours at RT on rotator. -PBS + 1 % NDS + 0.3 % triton X 100 + anti-rabbit Cy3 (Jackson Immunoresearch Labs) at 1:60011) Perform 3 X 10 minute PBS washes (1 mL volume) on rotator12) Mount tissue sections on gelatin coated slides and allow tissue to dry13) Run slides through alcohol gradients (70 %, 90 %, 100 %, xylene) leaving slide in each alcohol and xylene for 2 minutes14) Coverslip with DPX mountant (FLUKA) ** Note: 24 well plates may be substituted for microcentrifuge tubes. Use flat top bench rotator instead of upright rotator.
|Restrictions||For Research Use only|
HandlingProduct Details anti-TACR1 Antibody Target Details TACR1 Application Details References for anti-TACR1 antibody (ABIN152451) Images back to top
Buffer contains: No Preservative
|Handling Advice||Avoid freeze-thaw cycles|
|Storage||-80 °C,-20 °C|
|Storage Comment||Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.|
References for anti-TACR1 antibody (ABIN152451)Product Details anti-TACR1 Antibody Target Details TACR1 Application Details Handling Images back to top
|Product cited in:||
Li, Guo, Sun, Li, Ma, Zhang, Guan, Li, Wang: "Botulinum toxin type A and gabapentin attenuate postoperative pain and NK1 receptor internalization in rats." in: Neurochemistry international, Vol. 116, pp. 52-62, 2018
Morinaga, Nakamuta, Yamamoto: "Hypoxia-induced increases in serotonin-immunoreactive nerve fibers in the medulla oblongata of the rat." in: Acta histochemica, Vol. 118, Issue 8, pp. 806-817, 2016 (Sample species: Human).
Wu, Zhang, Qian, Guo, Yang, Huang, Luo, Xiang, Deng, Dai, Peng, Pan: "Antinociceptive effects of endomorphin-2: suppression of substance P release in the inflammatory pain model rat." in: Neurochemistry international, Vol. 82, pp. 1-9, 2015 (Sample species: Rat (Rattus)). Further details: Immunohistochemistry (Frozen Sections)
Li, Fotinos, Wu, Chen, Zhu, Baranov, Tu, Zhou, Sinha, Kristal, Wang: "N-acetyl-L-tryptophan delays disease onset and extends survival in an amyotrophic lateral sclerosis transgenic mouse model." in: Neurobiology of disease, Vol. 80, pp. 93-103, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting
Grip, Lonne-Rahm, Holst, Johansson, Nordlind, Theodorsson, El-Nour: "Substance P alterations in skin and brain of chronically stressed atopic-like mice." in: Journal of the European Academy of Dermatology and Venereology : JEADV, Vol. 27, Issue 2, pp. 199-205, 2013 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry
Ramalho, Almeida, Fernandes, Costa, Pirraco, Guardão, Delgado, Moreira, Soares: "Neurokinin-1 receptor, a new modulator of lymphangiogenesis in obese-asthma phenotype." in: Life sciences, Vol. 93, Issue 4, pp. 169-77, 2013 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)
Luo, Li, Zhang, Zheng, Wang, Nie, Zhang, Su: "Effects of four antitussives on airway neurogenic inflammation in a guinea pig model of chronic cough induced by cigarette smoke exposure." in: Inflammation research : official journal of the European Histamine Research Society ... [et al.], Vol. 62, Issue 12, pp. 1053-61, 2013 (Sample species: Guinea Pig). Further details: Western Blotting
Yang, Yan, Zhang, Wang: "Substance P participates in immune-mediated hepatic injury induced by concanavalin A in mice and stimulates cytokine synthesis in Kupffer cells." in: Experimental and therapeutic medicine, Vol. 6, Issue 2, pp. 459-464, 2013 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence
Lin, Moore, Jones, McGlashon, Talman: "Astrocytes in the rat nucleus tractus solitarii are critical for cardiovascular reflex control." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 47, pp. 18608-17, 2013 (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunohistochemistry (Paraffin-embedded Sections),Immunofluorescence
Talman, Dragon, Jones, Moore, Lin: "Sudden death and myocardial lesions after damage to catecholamine neurons of the nucleus tractus solitarii in rat." in: Cellular and molecular neurobiology, Vol. 32, Issue 7, pp. 1119-26, 2012 (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen Sections)
Caioli, Curcio, Pieri, Antonini, Marolda, Severini, Zona: "Substance P receptor activation induces downregulation of the AMPA receptor functionality in cortical neurons from a genetic model of Amyotrophic Lateral Sclerosis." in: Neurobiology of disease, Vol. 44, Issue 1, pp. 92-101, 2011 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence
Commons: "Locally collateralizing glutamate neurons in the dorsal raphe nucleus responsive to substance P contain vesicular glutamate transporter 3 (VGLUT3)." in: Journal of chemical neuroanatomy, Vol. 38, Issue 4, pp. 273-81, 2009 (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry
Amadoro, Pieri, Ciotti, Carunchio, Canu, Calissano, Zona, Severini: "Substance P provides neuroprotection in cerebellar granule cells through Akt and MAPK/Erk activation: evidence for the involvement of the delayed rectifier potassium current." in: Neuropharmacology, Vol. 52, Issue 6, pp. 1366-77, 2007 (Sample species: Rat (Rattus)).
Broccardo, Linari, Agostini, Amadoro, Carpino, Ciotti, Petrella, Petrozza, Severini, Improta: "Expression of NK-1 and NK-3 tachykinin receptors in pancreatic acinar cells after acute experimental pancreatitis in rats." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 291, Issue 3, pp. G518-24, 2006 (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence
Broccardo, Ciotti, Linari, Agostini, Petrella, Amadoro, Severini, Improta: "Immunocytochemical distribution of NK-1 and NK-3 tachykinin receptors in isolated pancreatic acini of guinea pigs and rats." in: Peptides, Vol. 26, Issue 11, pp. 2351-4, 2005
Commons, Connolley, Valentino: "A neurochemically distinct dorsal raphe-limbic circuit with a potential role in affective disorders." in: Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, Vol. 28, Issue 2, pp. 206-15, 2003 (Sample species: Rat (Rattus)). Further details: Immunocytochemistry,Immunofluorescence
Mounir, Parent: "The expression of neurokinin-1 receptor at striatal and pallidal levels in normal human brain." in: Neuroscience research, Vol. 44, Issue 1, pp. 71-81, 2002 (Sample species: Human). Further details: Western Blotting,Immunohistochemistry
Ma, Huang, Chien, Wu, Chen: "Temporal decrease in renal sensory responses in rats after chronic ligation of the bile duct." in: American journal of physiology. Renal physiology, Vol. 283, Issue 1, pp. F164-72, 2002 (Sample species: Rat (Rattus)). Further details: Western Blotting
Kc, Haxhiu, Tolentino-Silva, Wu, Trouth, Mack: "Paraventricular vasopressin-containing neurons project to brain stem and spinal cord respiratory-related sites." in: Respiratory physiology & neurobiology, Vol. 133, Issue 1-2, pp. 75-88, 2002
Yip, Chahl: "Localization of NK1 and NK3 receptors in guinea-pig brain." in: Regulatory peptides, Vol. 98, Issue 1-2, pp. 55-62, 2001 (Sample species: Guinea Pig). Further details: Immunohistochemistry
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