anti-Dog (Canine) Nucleolin antibody for Western Blotting

Recommended Nucleolin Antibody (supplied by: Log in to see )

Antigen
Nucleolin (NCL) Antibodies
  • C23
  • B530004O11Rik
  • D0Nds28
  • D1Nds28
  • Nucl
  • wu:fa12d03
  • zgc:152810
  • nucl
  • LOC100282923
  • LOC100283058
  • nucleolin
  • nucleolin S homeolog
  • NCL
  • Ncl
  • ncl.S
  • ncl
  • LOC100195537
  • LOC100282923
  • LOC100283058
Epitope
C-Term
16
14
13
12
11
8
8
8
7
7
5
3
3
3
3
2
2
2
2
2
1
1
1
1
1
Reactivity
Human, Rat (Rattus), Dog (Canine)
232
56
37
12
11
10
10
10
7
7
6
5
3
1
1
1
1
1
1
1
Host
Rabbit
125
115
1
Clonality
Polyclonal
Conjugate
This Nucleolin antibody is un-conjugated
16
11
10
9
9
9
3
3
3
3
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
217
135
124
91
63
58
41
24
21
17
13
9
2
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see

Available images

Catalog No. ABIN629901
$ 388.93
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
16.8439 ABIN2778936 IHC WB Rabbit C-Term Log in to see Polyclonal 1
13.843901 ABIN2778935 IHC WB Rabbit N-Term Log in to see Polyclonal 1
7 ABIN202879 IHC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
4.8439007 ABIN2462187 IHC ELISA WB Rabbit Log in to see Polyclonal 0
1 ABIN6290705 WB Goat IgG C-Term Log in to see Polyclonal 0
1 ABIN1916332 IF IHC IHC (p) IP WB Rabbit IgG AA 550-710, C-Term Log in to see Polyclonal 0
1 ABIN1916330 IF IHC IHC (p) IP WB Rabbit IgG AA 250-300 Log in to see Polyclonal 0
1 ABIN1916331 IF IHC IHC (p) IP WB Rabbit IgG AA 525-575 Log in to see Polyclonal 0
1 ABIN301790 ICC IP ELISA WB Mouse IgG1, kappa Log in to see 0
1 ABIN6731026 WB Mouse IgG2a Log in to see 0

General

Antigen Nucleolin (NCL) Antibodies
Epitope C-Term
(16), (14), (13), (12), (11), (8), (8), (8), (7), (7), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus), Dog (Canine)
(232), (56), (37), (12), (11), (10), (10), (10), (7), (7), (6), (5), (3), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(125), (115), (1)
Clonality
Polyclonal
Conjugate This Nucleolin antibody is un-conjugated
(16), (11), (10), (9), (9), (9), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(217), (135), (124), (91), (63), (58), (41), (24), (21), (17), (13), (9), (2), (1), (1)
Supplier Log in to see

Product Details anti-Nucleolin Antibody

Target Details Nucleolin Application Details Handling Images
Specificity Nucleolin antibody was raised against the C terminal of NCL
Purification Purified
Immunogen Nucleolin antibody was raised using the C terminal of NCL corresponding to a region with amino acids GGFGGRGGGRGGRGGFGGRGRGGFGGRGGFRGGRGGGGDHKPQGKKTKFE
Plasmids, Primers & others

Target Details Nucleolin

Product Details anti-Nucleolin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Nucleolin (NCL Antibody Abstract)
Background Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
Molecular Weight 78 kDa (MW of target protein)
Pathways Ribonucleoprotein Complex Subunit Organization

Application Details

Product Details anti-Nucleolin Antibody Target Details Nucleolin Handling Images back to top
Application Notes WB: 1.25 µg/mL, IHC: 4-8 µg/mL
Optimal conditions should be determined by the investigator.
Comment

Nucleolin Blocking Peptide, catalog no. 33R-3278, is also available for use as a blocking control in assays to test for specificity of this Nucleolin antibody

Restrictions For Research Use only

Handling

Product Details anti-Nucleolin Antibody Target Details Nucleolin Application Details Images back to top
Format Lyophilized
Reconstitution Lyophilized powder. Add distilled water for a 1 mg/mL concentration of NCL antibody in PBS
Concentration Lot specific
Buffer PBS
Handling Advice Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.
Storage 4 °C
Storage Comment Store at 2-8 °C for short periods. For longer periods of storage, store at -20 °C.

Images

Product Details anti-Nucleolin Antibody Target Details Nucleolin Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Nucleolin (NCL) (C-Term) antibody (ABIN629901) Nucleolin antibody was used for immunohistochemistry at a concentration of 4-8 ug/ml ...
Western Blotting (WB) image for anti-Nucleolin (NCL) (C-Term) antibody (ABIN629901) Nucleolin antibody used at 1.25 ug/ml to detect target protein.