anti-Rat (Rattus) Nucleolin antibody for ELISA

Recommended Nucleolin Antibody (supplied by: Log in to see )

Antigen
Nucleolin (NCL) Antibodies
  • C23
  • B530004O11Rik
  • D0Nds28
  • D1Nds28
  • Nucl
  • wu:fa12d03
  • zgc:152810
  • nucl
  • LOC100282923
  • LOC100283058
  • nucleolin
  • nucleolin S homeolog
  • NCL
  • Ncl
  • ncl.S
  • ncl
  • LOC100195537
  • LOC100282923
  • LOC100283058
Reactivity
Dog (Canine), Human, Rat (Rattus)
232
56
37
12
11
10
10
10
7
7
6
5
3
1
1
1
1
1
1
1
Host
Rabbit
125
115
1
Clonality
Polyclonal
Conjugate
This Nucleolin antibody is un-conjugated
16
11
10
9
9
9
3
3
3
3
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
217
135
124
91
63
57
41
24
21
17
13
9
2
1
1
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Supplier
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Available images

Catalog No. ABIN2462187
$ 446.88
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5873227 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5873226 ELISA IHC WB Rabbit Log in to see Polyclonal 0

General

Antigen Nucleolin (NCL) Antibodies
Reactivity Dog (Canine), Human, Rat (Rattus)
(232), (56), (37), (12), (11), (10), (10), (10), (7), (7), (6), (5), (3), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(125), (115), (1)
Clonality
Polyclonal
Conjugate This Nucleolin antibody is un-conjugated
(16), (11), (10), (9), (9), (9), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(217), (135), (124), (91), (63), (57), (41), (24), (21), (17), (13), (9), (2), (1), (1)
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Product Details anti-Nucleolin Antibody

Target Details Nucleolin Application Details Handling Images
Purification Antibody is purified by protein A chromatography method.
Immunogen Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NCL.
Plasmids, Primers & others

Target Details Nucleolin

Product Details anti-Nucleolin Antibody Application Details Handling Images back to top
Antigen
Alternative Name NCL (NCL Antibody Abstract)
Background Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus.Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
Molecular Weight 78 kDa
Gene ID 4691
NCBI Accession NP_005372
UniProt P19338
Pathways Ribonucleoprotein Complex Subunit Organization

Application Details

Product Details anti-Nucleolin Antibody Target Details Nucleolin Handling Images back to top
Application Notes NCL antibody can be used for detection of NCL by ELISA at 1:312500. NCL antibody can be used for detection of NCL by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Restrictions For Research Use only

Handling

Product Details anti-Nucleolin Antibody Target Details Nucleolin Application Details Images back to top
Format Lyophilized
Reconstitution Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Concentration 1 mg/mL
Buffer Antibody is lyophilized in PBS buffer with 2 % sucrose.
Handling Advice As with any antibody avoid repeat freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment For short periods of storage (days) store at 4 °C. For longer periods of storage, store NCL antibody at -20 °C.

Images

Product Details anti-Nucleolin Antibody Target Details Nucleolin Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Nucleolin (NCL) antibody (ABIN2462187) anti-Nucleolin (NCL) antibody