anti-Mouse (Murine) Methionine Adenosyltransferase II, alpha antibody for Immunocytochemistry

Recommended Methionine Adenosyltransferase II, alpha Antibody (supplied by: Log in to see )

Antigen
Methionine Adenosyltransferase II, alpha (MAT2A) Antibodies
  • MATA2
  • MATII
  • SAMS2
  • Sams2
  • D630045P18Rik
  • fd12a12
  • mat2a
  • si:ch73-340n8.1
  • wu:fb95e01
  • wu:fd12a12
  • m(2)21ab
  • MGC76253
  • MGC79598
  • zgc:110847
  • methionine adenosyltransferase 2A
  • methionine adenosyltransferase II, alpha
  • methionine adenosyltransferase II, alpha a
  • methionine adenosyltransferase II, alpha L homeolog
  • methionine adenosyltransferase II, alpha b
  • MAT2A
  • Mat2a
  • mat2aa
  • mat2a.L
  • mat2a
  • mat2ab
Epitope
N-Term, AA 1-100
16
13
9
7
6
5
2
2
1
1
Reactivity
Cow (Bovine), Human, Mouse (Murine), Primate, Rat (Rattus)
63
12
12
3
3
2
2
2
2
1
1
1
1
1
Host
Rabbit
49
16
2
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
3
2
2
2
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
60
30
14
10
5
4
1
1
Options
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Available images

Catalog No. ABIN410043
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
-11.123236 ABIN410044 ICC IF WB Biotin Rabbit N-Term, AA 1-100 Log in to see Polyclonal 0
-11.123236 ABIN410046 ICC IF WB DyLight 488 Rabbit N-Term, AA 1-100 Log in to see Polyclonal 0
-11.123236 ABIN4332800 ICC IF WB Alexa Fluor 488 Rabbit AA 1-100, N-Term Log in to see Polyclonal 0
-11.123236 ABIN4332801 ICC IF WB Alexa Fluor 647 Rabbit AA 1-100, N-Term Log in to see Polyclonal 0
-11.123236 ABIN4332802 ICC IF WB Alexa Fluor 700 Rabbit AA 1-100, N-Term Log in to see Polyclonal 0
-11.123236 ABIN4332793 ICC IF WB DyLight 650 Rabbit AA 1-100, N-Term Log in to see Polyclonal 0
-11.123236 ABIN4332794 ICC IF WB DyLight 550 Rabbit AA 1-100, N-Term Log in to see Polyclonal 0
-11.123236 ABIN4332797 ICC IF FITC Rabbit AA 1-100, N-Term Log in to see Polyclonal 0
-11.123236 ABIN4332798 ICC IF WB DyLight 405 Rabbit AA 1-100, N-Term Log in to see Polyclonal 0
-11.123236 ABIN4332799 ICC IF WB Alexa Fluor 405 Rabbit AA 1-100, N-Term Log in to see Polyclonal 0

General

Antigen Methionine Adenosyltransferase II, alpha (MAT2A) Antibodies
Epitope N-Term, AA 1-100
(16), (13), (9), (7), (6), (5), (2), (2), (1), (1)
Reactivity Cow (Bovine), Human, Mouse (Murine), Primate, Rat (Rattus)
(63), (12), (12), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(49), (16), (2)
Clonality
Polyclonal
Conjugate Un-conjugated
(4), (4), (3), (2), (2), (2), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(60), (30), (14), (10), (5), (4), (1), (1)
Pubmed 6 references available
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Product details

Target Details Application Details Handling References Images
Purification Immunogen affinity purified
Immunogen Synthetic peptide made to an N-terminal portion of human MAT2A (within residues 1-100). [Swiss-Prot# P31153]

Target Details

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Antigen
Alternative Name MAT2A (MAT2A Antibody Abstract)
Background Gene Symbol: MAT2A
Molecular Weight Theoretical MW: 43 kDa
Gene ID 4144
UniProt P31153
Pathways Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process

Application Details

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Application Notes Western Blot 1.0 μg/mL, Immunocytochemistry/Immunofluorescence 1:2000This MAT2A antibody is useful for Western blot, where a band is seen at ~43 kDa, and Immunocytochemistry/Immunofluorescence where cytoplasmic staining is observed in HepG2 cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for MAT2A Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 % MOPS) on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % NFDM + 1 % BSA in TBS + Tween, 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-MAT2a primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturersinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.Immunocytochemistry Protocol Immunocytochemistry ProtocolCulture cells to appropriate density in 35 mm culture dishes or 6-well plates.
. Remove culture medium and add 10 % formalin to the dish. Fix at room temperature for 30 minutes.
. Remove the formalin and add ice cold methanol. Incubate for 5-10 minutes.
. Remove methanol and add washing solution (i.e. PBS). Be sure to not let the specimen dry out. Wash three times for 10 minutes.
. To block nonspecific antibody binding incubate in 10 % normal goat serum from 1 hour to overnight at room temperature.
. Add primary antibody at appropriate dilution and incubate at room temperature from 2 hours to overnight at room temperature.
. Remove primary antibody and replace with washing solution. Wash three times for 10 minutes.
. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
. Remove antibody and replace with wash solution, then wash for 10 minutes. Add Hoechst 33258 to wash solution at 1:25,0000 and incubate for 10 minutes. Wash a third time for 10 minutes.
. Cells can be viewed directly after washing. The plates can also be stored in PBS containing Azide covered in Parafilm (TM). Cells can also be cover-slipped using Fluoromount, with appropriate sealing.*The above information is only intended as a guide. The researcher should determine what protocol best meets their needs. Please follow safe laboratory procedures.
Restrictions For Research Use only

Handling

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Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References

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Product cited in:

Stone, El-Brolosy, Wilhelm, Liu, Romão, Grillo, Lai, Günther, Jeratsch, Kuenne, Lee, Braun, Santoro, Locasale, Potente, Stainier: "Loss of pyruvate kinase M2 limits growth and triggers innate immune signaling in endothelial cells." in: Nature communications, Vol. 9, Issue 1, pp. 4077, 2019 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Tomasi, Cossu, Spissu, Floris, Ryoo, Iglesias-Ara, Wang, Pandol, Bhowmick, Seki, Posadas, Lu: "S-adenosylmethionine and methylthioadenosine inhibit cancer metastasis by targeting microRNA 34a/b-methionine adenosyltransferase 2A/2B axis." in: Oncotarget, Vol. 8, Issue 45, pp. 78851-78869, 2018 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Zhong, Liu, Lin, Hu, Yuan, Liu, Zhou, Xu, Hu, Wang, Guan, Hao, Lui, Guo, Mai, Chen, Han, Xia, Grandis, Zhang, Zeng: "The RARS-MAD1L1 Fusion Gene Induces Cancer Stem Cell-like Properties and Therapeutic Resistance in Nasopharyngeal Carcinoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 24, Issue 3, pp. 659-673, 2018 Method employed by authors: Western Blotting (WB) (Sample species: Pig (Porcine)).

Scotton, Bovolenta, Schwartz, Falzarano, Martoni, Passarelli, Armaroli, Osman, Rodolico, Messina, Pegoraro, DAmico, Bertini, Gualandi, Neri, Selvatici, Boffi, Maioli, Lochmüller, Straub, Bushby et al.: "Deep RNA profiling identified CLOCK and molecular clock genes as pathophysiological signatures in collagen VI myopathy. ..." in: Journal of cell science, Vol. 129, Issue 8, pp. 1671-84, 2016 Further details: Western Blotting

Ramani, Donoyan, Tomasi, Park: "Role of methionine adenosyltransferase α2 and β phosphorylation and stabilization in human hepatic stellate cell trans-differentiation." in: Journal of cellular physiology, Vol. 230, Issue 5, pp. 1075-85, 2015 (Sample species: Human). Further details: Immunoprecipitation

Ramani, Tomasi: "Transcriptional regulation of methionine adenosyltransferase 2A by peroxisome proliferator-activated receptors in rat hepatic stellate cells." in: Hepatology (Baltimore, Md.), Vol. 55, Issue 6, pp. 1942-53, 2012 (Sample species: Mouse (Murine)). Further details: Western Blotting

Images

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Supplier Images
Western Blotting (WB) image for anti-Methionine Adenosyltransferase II, alpha (MAT2A) (AA 1-100), (N-Term) antibody (ABIN410043) Western Blot: MAT2A Antibody [ABIN4100438] - Total protein from HeLa and HepG2 cells ...
Immunofluorescence (IF) image for anti-Methionine Adenosyltransferase II, alpha (MAT2A) (AA 1-100), (N-Term) antibody (ABIN410043) Immunocytochemistry/Immunofluorescence: MAT2A Antibody [ABIN4100438] - MAT2A antibody...
Western Blotting (WB) image for anti-Methionine Adenosyltransferase II, alpha (MAT2A) (AA 1-100), (N-Term) antibody (ABIN410043) Western Blot: MAT2A Antibody [ABIN4100438] - Detection of MAT2A in HepG2 whole cell l...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Methionine Adenosyltransferase II, alpha (MAT2A) (AA 1-100), (N-Term) antibody (ABIN410043) Immunocytochemistry/Immunofluorescence: MAT2A Antibody - Mouse embryonic stem cells ...