anti-Monkey AKT antibody for Western Blotting

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • CWS6
  • PKB
  • PKB-ALPHA
  • PRKBA
  • RAC
  • RAC-ALPHA
  • Akt
  • Rac
  • PKB/Akt
  • PKBalpha
  • AKT/PKB
  • AKT1
  • Akt/PKB
  • Akt1
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/dAKT
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ACT-5
  • akt-1
  • pkb
  • v-akt
  • v-akt1
  • xAct
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • AKT serine/threonine kinase 1
  • thymoma viral proto-oncogene 1
  • CG4006 gene product from transcript CG4006-RE
  • actin beta
  • v-akt murine thymoma viral oncogene homolog 1 S homeolog
  • v-akt murine thymoma viral oncogene homolog 1
  • K+ transporter 1
  • Serine/threonine-protein kinase akt-1
  • AKT1
  • Akt1
  • ACTB
  • akt1.S
  • akt1
  • KT1
  • akt-1
Epitope
pSer473, Internal Region
215
112
108
58
57
48
37
32
29
26
25
21
19
18
18
18
14
13
13
12
12
12
12
12
10
10
9
9
8
7
7
6
6
6
6
6
5
5
5
5
5
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus), Monkey
1305
745
602
68
55
54
52
44
31
29
6
6
6
5
4
3
3
3
3
1
1
1
1
Host
Mouse
1032
356
11
4
2
2
Clonality
Monoclonal
Conjugate
This AKT antibody is conjugated to Biotin
54
43
39
39
31
26
12
12
7
7
7
5
5
5
5
5
5
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
1209
621
600
283
205
195
133
110
61
38
32
26
12
11
10
4
4
3
3
2
2
1
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Pubmed
4 references available

Available images

image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752)
image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752)
image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752)
image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752)
ELISA image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752)
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752)
Catalog No. ABIN1043752
$ 450.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
35.892227 ABIN1043753 IP IHC ELISA WB Biotin Mouse IgG1 kappa pThr308, Internal Region Log in to see 18F3-H11 4
31.392225 ABIN964561 IP IHC ELISA WB Mouse IgG1 kappa pSer473 Log in to see 17F6-B11 0
31.392225 ABIN5542263 ELISA FACS ICC IHC WB Mouse IgG1 AA 1-150 Log in to see 1F7E10 0
31.392225 ABIN5542264 ELISA FACS ICC IHC WB Mouse IgG1 AA 1-150 Log in to see 1F7G2 0
26.892225 ABIN1043756 FM FRET STED ELISA WB Atto 594 Mouse IgG1 kappa pSer473, Internal Region Log in to see 17F6-B11 4
23.892225 ABIN1043729 IP IHC ELISA WB Mouse IgG1 kappa pThr308 Log in to see 18F3-H11 4
22.392225 ABIN3182568 ELISA IHC WB Rabbit IgG pTyr474 Log in to see Polyclonal 0
20.892225 ABIN968951 ELISA WB Mouse IgG1 Log in to see 3A3 3
19.819988 ABIN4880525 ELISA FACS ICC WB Mouse IgG1 AA 1-150 Log in to see 1F7E10 0
19.819988 ABIN4880526 ELISA FACS IHC WB Mouse IgG1 AA 1-150 Log in to see 1F7G2 0
19.392225 ABIN349626 IP IHC ELISA WB Mouse IgG1 kappa pThr308 Log in to see 18F3-H11 0
19.392225 ABIN3181908 ELISA IHC WB Rabbit IgG pThr308 Log in to see Polyclonal 0
19.392225 ABIN3183233 ELISA IF IHC WB Rabbit IgG Ser13 Log in to see Polyclonal 0
19 ABIN1496555 FACS IF IHC IHC (p) WB Mouse IgG1 Log in to see 3E3 0
19 ABIN1496558 FACS IF IHC IHC (p) WB Mouse IgG1 Log in to see 4C11 0
19 ABIN1496557 FACS IF IHC IHC (p) WB Mouse IgG1 Log in to see 4E10 0
19 ABIN1496554 FACS IF IHC IHC (p) WB Mouse IgG1 Log in to see 4D6 0
19 ABIN1099896 ELISA IF IHC IHC (p) WB Mouse IgG1 pSer473 Log in to see 17F6-B11 0
19 ABIN1496556 FACS IF IHC IHC (p) WB Mouse IgG1 Log in to see 3B4 0
17.072239 ABIN965527 ELISA WB Mouse IgG1 Log in to see 3A3 3

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope pSer473, Internal Region
(215), (112), (108), (58), (57), (48), (37), (32), (29), (26), (25), (21), (19), (18), (18), (18), (14), (13), (13), (12), (12), (12), (12), (12), (10), (10), (9), (9), (8), (7), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus), Monkey
(1305), (745), (602), (68), (55), (54), (52), (44), (31), (29), (6), (6), (6), (5), (4), (3), (3), (3), (3), (1), (1), (1), (1)
Host Mouse
(1032), (356), (11), (4), (2), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This AKT antibody is conjugated to Biotin
(54), (43), (39), (39), (31), (26), (12), (12), (7), (7), (7), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(1209), (621), (600), (283), (205), (195), (133), (110), (61), (38), (32), (26), (12), (11), (10), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
Supplier Log in to see

Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT antibody (ABIN1043752) Images
Specificity Anti-AKT pS473 (MOUSE) Monoclonal Antibody Biotin Conjugated was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human and mouse AKT protein phosphorylated at S473. A BLAST analysis was used to suggest cross-reactivity with AKT pS473 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 has not been determined.
Characteristics AKT phospho 473 is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. Anti-AKT pS473 (MOUSE) Monoclonal Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research.
Immunogen Anti-AKT pS473 (MOUSE) Monoclonal Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein.
Immunogen Type: Peptide
Clone 17F6-B11
Isotype IgG1 kappa
Plasmids, Primers & others

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT antibody (ABIN1043752) Images back to top
Antigen
Alternative Name Akt (AKT1 Antibody Abstract)
Background AKT phospho 473 is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT pS473 (MOUSE) Monoclonal Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research.
Synonyms: phospho AKT, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1, phospho AKT antibody, Anti-AKT pS473 Antibody Biotin Conjugated
Gene ID 207, 62241011
UniProt P31749
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT antibody (ABIN1043752) Images back to top
Application Notes Biotin Conjugated Anti-AKT pS473 (MOUSE) Monoclonal Antibody Biotin Conjugated is suitable for ELISA, immunohistochemistry, immunoprecipitation and western blotting. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry use formalin-fixed paraffin-embedded sections. No pre-treatment of sample is required.
Comment

Gene Name: AKT1
Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT antibody (ABIN1043752) Images back to top
Format Lyophilized
Reconstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Expiry Date 12 months

References for anti-AKT antibody (ABIN1043752)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Product cited in:

Lawlor, Alessi: "PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?" in: Journal of cell science, Vol. 114, Issue Pt 16, pp. 2903-10, 2001

Alessi: "Discovery of PDK1, one of the missing links in insulin signal transduction. Colworth Medal Lecture." in: Biochemical Society transactions, Vol. 29, Issue Pt 2, pp. 1-14, 2001

Jones, Jakubowicz, Pitossi, Maurer, Hemmings: "Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 10, pp. 4171-5, 1991

Staal: "Molecular cloning of the akt oncogene and its human homologues AKT1 and AKT2: amplification of AKT1 in a primary human gastric adenocarcinoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 14, pp. 5034-7, 1987

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT antibody (ABIN1043752) back to top
Supplier Images
image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin)
image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (Image 2)
image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (Image 3)
image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (Image 4)
ELISA image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752) ELISA of Mouse anti-Akt phospho S473 Biotin Conjugated antibody. Antigen: BSA conjuga...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752) Western Blot of Mouse anti-Akt phospho S473 Biotin Conjugated antibody. Lane 1: GST t...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752) Western Blot of Mouse anti-Akt phospho S473 Biotin Conjugated antibody. Lane 1: GST t...
Immunohistochemistry (IHC) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752) Immunohistochemistry of mouse anti AKT phospho S473 biotin conjugated at 40X (left) w...
Immunohistochemistry (IHC) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (Internal Region), (pSer473) antibody (Biotin) (ABIN1043752) Immunohistochemistry of mouse Anti-AKT pS473 (MOUSE) Biotin Conjugated  at 40X Tissue...