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Submit your validation data for this product and get a full refund. I want to validate this productRelevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
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3.2908103 | ABIN1043752 | IP IHC ELISA WB | Biotin | Mouse | IgG1 kappa | pSer473, Internal Region | Log in to see | 17F6-B11 | 4 | |
3.2908103 | ABIN964560 | IP IHC ELISA WB | Mouse | IgG1 kappa | pSer473 | Log in to see | 17F6-B11 | 0 | ||
3.2908103 | ABIN1043756 | FM FRET STED ELISA WB | Atto 594 | Mouse | IgG1 kappa | pSer473, Internal Region | Log in to see | 17F6-B11 | 4 | |
3.2908103 | ABIN2704460 | IHC WB | Rabbit | C-Term, pTyr474 | Log in to see | Polyclonal | 0 | |||
3.2908103 | ABIN2707319 | IHC WB | Rabbit | N-Term | Log in to see | Polyclonal | 0 | |||
3.2908103 | ABIN1043729 | IP IHC ELISA WB | Mouse | IgG1 kappa | pThr308 | Log in to see | 18F3-H11 | 4 | ||
3.2908103 | ABIN4279004 | FACS ICC IF IHC IHC (p) WB | Mouse | IgG1 | Log in to see | 4D6 | 1 | |||
3.2908103 | ABIN964561 | IP IHC ELISA WB | Mouse | IgG1 kappa | pSer473 | Log in to see | 17F6-B11 | 0 | ||
3.2908103 | ABIN964562 | IP IHC ELISA WB | Mouse | IgG1 kappa | pThr308 | Log in to see | 18F3-H11 | 0 | ||
3.2908103 | ABIN3182568 | ELISA IHC WB | Rabbit | IgG | pTyr474 | Log in to see | Polyclonal | 0 | ||
3.2908103 | ABIN2455513 | FACS IF IHC WB | Mouse | IgG1 | Log in to see | 4C11 | 0 | |||
3.2908103 | ABIN2455512 | FACS IF IHC WB | Mouse | IgG1 | Log in to see | 4E10 | 1 | |||
3.2908103 | ABIN968951 | ELISA WB | Mouse | IgG1 | Log in to see | 3A3 | 3 | |||
3.2908103 | ABIN2455510 | FACS IF IHC WB | Mouse | IgG1 | Log in to see | 4G4 | 0 | |||
3.2908103 | ABIN2455509 | FACS IF IHC WB | Mouse | IgG1 | Log in to see | 3E3 | 0 | |||
3.2908103 | ABIN2455508 | FACS IF IHC WB | Mouse | IgG1 | Log in to see | 4D6 | 0 | |||
3.2908103 | ABIN2455511 | FACS IF IHC WB | Mouse | IgG1 | Log in to see | 3B4 | 0 | |||
3.2908103 | ABIN3181908 | ELISA IHC WB | Rabbit | IgG | pThr308 | Log in to see | Polyclonal | 0 | ||
3.2908103 | ABIN3183233 | ELISA IF IHC WB | Rabbit | IgG | Ser13 | Log in to see | Polyclonal | 0 | ||
3.2908103 | ABIN4279007 | ELISA FACS IHC IHC (p) WB | Biotin | Mouse | IgG1 kappa | pThr308 | Log in to see | 18F3-H11 | 0 |
General |
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Antigen | V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies |
Epitope | pThr308, Internal Region Alternatives |
Reactivity | Human, Mouse (Murine), Rat (Rattus), Monkey Alternatives |
Host | Mouse Alternatives |
Clonality (Clone) | |
Conjugate | This AKT antibody is conjugated to Biotin Alternatives |
Application |
Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
Alternatives
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4 references available |
Supplier | Log in to see |
Product Details anti-AKT AntibodyTarget Details AKT Application Details Handling References for anti-AKT antibody (ABIN1043753) Images |
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Specificity | Anti-AKT pT308 was purified from concentrated tissue culture supernate by Protein A chromatography. This antibody is specific for human and mouse AKT protein phosphorylated at T308. A BLAST analysis was used to suggest cross-reactivity with AKT pT308 from most vertebrate species sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 will likely occur. Cancer, Cell Signaling, Neuroscience, Signal Transduction research. |
Characteristics | Anti-AKT phospho T308 is ideal for western blotting, ELISA, IHC and IP. Phospho AKT pT308 antibody is specific for AKT protein phosphorylated at T308. AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. Anti-AKT pT308 monoclonal antibody is ideal for investigators involved in Cancer, Cell Signaling, Neuroscience, Signal Transduction research. |
Immunogen |
Anti-AKT pT308 monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding T308 of human AKT1 protein. Immunogen Type: Peptide |
Clone | 18F3-H11 |
Isotype | IgG1 kappa |
Target Details AKTProduct Details anti-AKT Antibody Application Details Handling References for anti-AKT antibody (ABIN1043753) Images back to top |
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Antigen | |
Alternative Name | Akt (AKT1 Antibody Abstract) |
Background |
Anti-AKT phospho T308 is ideal for western blotting, ELISA, IHC and IP. Phospho AKT pT308 antibody is specific for AKT protein phosphorylated at T308. AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. Anti-AKT pT308 monoclonal antibody is ideal for investigators involved in Cancer, Cell Signaling, Neuroscience, Signal Transduction research. Synonyms: RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1, Akt phospho T308 Antibody, Anti-AKT pT308 Monoclonal Antibody Biotin Conjugated |
Gene ID | 207, 62241011 |
UniProt | P31749 |
Pathways | PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling |
Application DetailsProduct Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT antibody (ABIN1043753) Images back to top |
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Application Notes | Biotin Conjugated Anti-AKT pT308 is suitable for ELISA, immunohistochemistry, immunoprecipitation and western blotting. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pT308 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. Use formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required. |
Comment |
Gene Name: AKT1 |
Restrictions | For Research Use only |
HandlingProduct Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT antibody (ABIN1043753) Images back to top |
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Format | Lyophilized |
Reconstitution | Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL |
Concentration | 1.0 mg/mL |
Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free |
Preservative | Sodium azide |
Precaution of Use | This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Storage | 4 °C/-20 °C |
Storage Comment | Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. |
Expiry Date | 12 months |
References for anti-AKT antibody (ABIN1043753)Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top |
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Product cited in: |
Lawlor, Alessi: "PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?" in: Journal of cell science, Vol. 114, Issue Pt 16, pp. 2903-10, 2001 Alessi: "Discovery of PDK1, one of the missing links in insulin signal transduction. Colworth Medal Lecture." in: Biochemical Society transactions, Vol. 29, Issue Pt 2, pp. 1-14, 2001 Jones, Jakubowicz, Pitossi, Maurer, Hemmings: "Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 10, pp. 4171-5, 1991 Staal: "Molecular cloning of the akt oncogene and its human homologues AKT1 and AKT2: amplification of AKT1 in a primary human gastric adenocarcinoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 14, pp. 5034-7, 1987 |
ImagesProduct Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT antibody (ABIN1043753) back to top |
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