anti-Rabbit EPH Receptor B2 antibody for Immunohistochemistry

Recommended EPH Receptor B2 Antibody (supplied by: Log in to see )

Antigen
EPH Receptor B2 (EPHB2) Antibodies
  • CAPB
  • DRT
  • EK5
  • EPHT3
  • ERK
  • Hek5
  • PCBC
  • Tyro5
  • Cek5
  • Drt
  • ETECK
  • Erk
  • Nuk
  • Prkm5
  • Qek5
  • Sek3
  • RGD1564232
  • EPH receptor B2
  • Eph receptor B2
  • EPHB2
  • Ephb2
Epitope
Internal Region
25
13
12
12
12
11
8
7
7
6
4
4
3
2
1
1
1
1
1
1
1
1
Reactivity
Human, Monkey, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
159
50
6
5
3
3
3
3
1
1
1
1
1
1
Host
Rabbit
91
40
20
18
Clonality
Polyclonal
Conjugate
This EPH Receptor B2 antibody is un-conjugated
10
7
7
7
7
6
2
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
128
99
60
38
31
29
15
7
3
2
1
1
1
1
1
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Catalog No. ABIN1048573
$ 484.00
Plus shipping costs $45.00

General

Antigen EPH Receptor B2 (EPHB2) Antibodies
Epitope Internal Region
(25), (13), (12), (12), (12), (11), (8), (7), (7), (6), (4), (4), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Monkey, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
(159), (50), (6), (5), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1)
Host Rabbit
(91), (40), (20), (18)
Clonality
Polyclonal
Conjugate This EPH Receptor B2 antibody is un-conjugated
(10), (7), (7), (7), (7), (6), (2), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(128), (99), (60), (38), (31), (29), (15), (7), (3), (2), (1), (1), (1), (1), (1)
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Product Details anti-EPH Receptor B2 Antibody

Target Details EPH Receptor B2 Application Details Handling Images
Brand IHC-plus™
Specificity Human EPHB2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Panda, Rabbit, Opossum, Zebrafish (100%) Hamster, Elephant, Bovine, Pig, Turkey, Chicken, Pufferfish (93%) Xenopus, Seabass (87%) Stickleback (80%).
Purification Immunoaffinity purified
Immunogen Synthetic 15 amino acid peptide from internal region of human EPHB2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Panda, Rabbit, Opossum, Zebrafish (100%), Hamster, Elephant, Bovine, Pig, Turkey, Chicken, Pufferfish (93%), Xenopus, Seabass (87%), Stickleback (80%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Target Details EPH Receptor B2

Product Details anti-EPH Receptor B2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name EPHB2 / EPH Receptor B2 (EPHB2 Antibody Abstract)
Background Name/Gene ID: EPHB2
Subfamily: Ephrin Receptor
Family: Protein Kinase

Synonyms: EPHB2, CAPB, Cek5, EK5, ETECK, EPH tyrosine kinase 3, DRT, ELK-related tyrosine kinase, Hek5, EPH-like kinase 5, EPHT3, Nuk, PCBC, Tyrosine-protein kinase TYRO5, Protein-tyrosine kinase HEK5, Tyro5, EPH receptor B2, Ephrin type-B receptor 2, EPTH3
Gene ID 2048
Pathways RTK Signaling, Regulation of long-term Neuronal Synaptic Plasticity

Application Details

Product Details anti-EPH Receptor B2 Antibody Target Details EPH Receptor B2 Handling Images back to top
Application Notes Approved: IHC, IHC-P (7 μg/mL)
Comment

Target Species of Antibody: Human

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

Product Details anti-EPH Receptor B2 Antibody Target Details EPH Receptor B2 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months

Images

Product Details anti-EPH Receptor B2 Antibody Target Details EPH Receptor B2 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-EPH Receptor B2 (EPHB2) (Internal Region) antibody (ABIN1048573) Anti-EPHB2 antibody ABIN1048573 IHC staining of human colon, carcinoma. Immunohistoc...