anti-Rat (Rattus) Ephrin B2 antibody for Western Blotting

Recommended Ephrin B2 Antibody (supplied by: Log in to see )

Antigen
Ephrin B2 (EFNB2) Antibodies
  • EFNB2
  • efnb2
  • ephrin-B2
  • htkl
  • eplg5
  • htk-l
  • lerk5
  • ephrinB2
  • efnb2a
  • MGC68890
  • efnb2b
  • ELF-2
  • Epl5
  • Eplg5
  • Htk-L
  • LERK-5
  • Lerk5
  • NLERK-1
  • EPLG5
  • HTKL
  • LERK5
  • ephrin B2
  • ephrin-B2 precursor
  • ephrin B2 L homeolog
  • ephrin B2 S homeolog
  • EFNB2
  • efnb2
  • efnb2.L
  • efnb2.S
  • Efnb2
Epitope
AA 27-227
15
11
11
11
10
5
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
103
46
26
5
5
5
3
3
3
2
2
1
1
Host
Goat
91
15
2
Clonality
Polyclonal
Conjugate
This Ephrin B2 antibody is un-conjugated
3
3
3
3
3
3
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Western Blotting (WB)
90
38
33
26
8
8
3
2
Supplier
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Catalog No. ABIN4900008
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2592473 ABIN2464896 ELISA WB Goat Internal Region Log in to see Polyclonal 0
3.2592473 ABIN4308729 ELISA WB Goat Log in to see Polyclonal 2
3.2592473 ABIN1876870 IHC WB Rabbit IgG Log in to see Polyclonal 0
3.2592473 ABIN3197628 WB Rabbit Log in to see Polyclonal 0
3.2592473 ABIN2562370 ELISA WB Goat IgG Internal Region Log in to see Polyclonal 0
3.2592473 ABIN274039 WB Rabbit IgG Log in to see Polyclonal 0
3.2592473 ABIN274041 IF WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN197418 IF WB Rabbit AA 328-332 Log in to see Polyclonal 3
1 ABIN5553985 EIA WB Goat Internal Region Log in to see Polyclonal 0
1 ABIN793161 ELISA WB Goat AA 62-74 Log in to see Polyclonal 0
1 ABIN362834 IF WB Rabbit IgG AA 328-332 Log in to see Polyclonal 4
1 ABIN362832 WB Rabbit IgG AA 314-318 Log in to see Polyclonal 3
1 ABIN5689271 WB Rabbit AA 314-318 Log in to see Polyclonal 0
1 ABIN1822404 WB Rabbit IgG pTyr316 Log in to see Polyclonal 0
1 ABIN1822405 WB Rabbit IgG pTyr330 Log in to see Polyclonal 0
1 ABIN594823 WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN594824 WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN571218 ELISA WB Goat Log in to see Polyclonal 0
1 ABIN2881211 IF IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2886957 IHC WB Rabbit Log in to see Polyclonal 0

General

Antigen Ephrin B2 (EFNB2) Antibodies
Epitope AA 27-227
(15), (11), (11), (11), (10), (5), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(103), (46), (26), (5), (5), (5), (3), (3), (3), (2), (2), (1), (1)
Host Goat
(91), (15), (2)
Clonality
Polyclonal
Conjugate This Ephrin B2 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Western Blotting (WB)
(90), (38), (33), (26), (8), (8), (3), (2)
Pubmed 16 references available
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Product Details anti-Ephrin B2 Antibody

Target Details Ephrin B2 Application Details Handling References for anti-Ephrin B2 antibody (ABIN4900008) Images
Specificity Detects mouse Ephrin-B2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5 % cross-reactivity with recombinant mouse Ephrin-B1 is observed.
Purification Antigen Affinity-purified
Sterility 0.2 μm filtered
Immunogen Mouse myeloma cell line NS0-derived recombinant mouse Ephrin-B2, Arg27-Ala227, Accession # AAA82934
Isotype IgG

Target Details Ephrin B2

Product Details anti-Ephrin B2 Antibody Application Details Handling References for anti-Ephrin B2 antibody (ABIN4900008) Images back to top
Antigen
Alternative Name Ephrin-B2 (EFNB2 Antibody Abstract)
Gene ID 1948
Pathways RTK Signaling, Regulation of Muscle Cell Differentiation

Application Details

Product Details anti-Ephrin B2 Antibody Target Details Ephrin B2 Handling References for anti-Ephrin B2 antibody (ABIN4900008) Images back to top
Application Notes Western Blot 1 μg/mL
Flow Cytometry 0.25 μg/10^6 cells
Immunocytochemistry 5-15 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-Ephrin B2 Antibody Target Details Ephrin B2 Application Details References for anti-Ephrin B2 antibody (ABIN4900008) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-Ephrin B2 antibody (ABIN4900008)

Product Details anti-Ephrin B2 Antibody Target Details Ephrin B2 Application Details Handling Images back to top
Product cited in:

Toray, Hasegawa, Sakagami, Tsuchiya, Kudo, Zhao, Moritani, Abe, Yoshida, Yamamoto, Yamamoto, Oda, Udagawa, Luiz de Freitas, Li: "Histochemical assessment for osteoblastic activity coupled with dysfunctional osteoclasts in c-src deficient mice." in: Biomedical research (Tokyo, Japan), Vol. 38, Issue 2, pp. 123-134, 2017 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).

Tsang, Hyun, Cheng, Vargas, Mehta, Ushio-Fukai, Zou, Pajcini, Rehman, Malik: "Embryonic Stem Cell Differentiation to Functional Arterial Endothelial Cells through Sequential Activation of ETV2 and NOTCH1 Signaling by HIF1α." in: Stem cell reports, Vol. 9, Issue 3, pp. 796-806, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Depner, Zum Buttel, Böğürcü, Cuesta, Aburto, Seidel, Finkelmeier, Foss, Hofmann, Kaulich, Barbus, Segarra, Reifenberger, Garvalov, Acker, Acker-Palmer: "EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance." in: Nature communications, Vol. 7, pp. 12329, 2016 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)).

Feng, Sakamoto, Wu, Liu, Wiese, Green, Green, Akyol, Roy, Zhai, Cho, Fearon: "Tissue-Specific Effects of Reduced β-catenin Expression on Adenomatous Polyposis Coli Mutation-Instigated Tumorigenesis in Mouse Colon and Ovarian Epithelium." in: PLoS genetics, Vol. 11, Issue 11, pp. e1005638, 2015 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)).

Salvucci, Ohnuki, Maric, Hou, Li, Yoon, Segarra, Eberhart, Acker-Palmer, Tosato: "EphrinB2 controls vessel pruning through STAT1-JNK3 signalling." in: Nature communications, Vol. 6, pp. 6576, 2015 Method employed by authors: Proximity Ligation Assay (PLA), Proximity Ligation Assay (PLA) (Sample species: Mouse (Murine)).

Dong, Gao, Wang, Miao, Wang, Wu, Li, Wu, Cheng, Sun, Yang, Wang: "GluA2 trafficking is involved in apoptosis of retinal ganglion cells induced by activation of EphB/EphrinB reverse signaling in a rat chronic ocular hypertension model." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 35, Issue 13, pp. 5409-21, 2015 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Rat (Rattus)).

Ji, Hwang, Mood, Cho, Lee, Winterbottom, Cousin, Daar: "EphrinB2 affects apical constriction in Xenopus embryos and is regulated by ADAM10 and flotillin-1." in: Nature communications, Vol. 5, pp. 3516, 2014 Method employed by authors: Immunoprecipitation (IP), Immunoprecipitation (IP) (Sample species: Human).

Conway, Schaffer: "Biomaterial microenvironments to support the generation of new neurons in the adult brain." in: Stem cells (Dayton, Ohio), Vol. 32, Issue 5, pp. 1220-9, 2014 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).

Kida, Ieronimakis, Schrimpf, Reyes, Duffield: "EphrinB2 reverse signaling protects against capillary rarefaction and fibrosis after kidney injury." in: Journal of the American Society of Nephrology : JASN, Vol. 24, Issue 4, pp. 559-72, 2013 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)).

Attwood, Bourgognon, Patel, Mucha, Schiavon, Skrzypiec, Young, Shiosaka, Korostynski, Piechota, Przewlocki, Pawlak: "Neuropsin cleaves EphB2 in the amygdala to control anxiety." in: Nature, Vol. 473, Issue 7347, pp. 372-5, 2011 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)).

He, Kumar, Zhou, Krasnoperov, Ryan, Gill, Hinton: "Soluble EphB4 inhibition of PDGF-induced RPE migration in vitro." in: Investigative ophthalmology & visual science, Vol. 51, Issue 1, pp. 543-52, 2010 Method employed by authors: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Frozen Sections) (IHC (fro)) (Sample species: Human).

Williams, Kim, Ni, Mitchell, Ro, Penn, Baldwin, Solnica-Krezel, Zhong: "Hedgehog signaling induces arterial endothelial cell formation by repressing venous cell fate." in: Developmental biology, Vol. 341, Issue 1, pp. 196-204, 2010 Method employed by authors: Immunohistochemistry (IHC), Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).

Zhang, Dong, Wang, Zhu, Croy: "Dynamic changes occur in patterns of endometrial EFNB2/EPHB4 expression during the period of spiral arterial modification in mice." in: Biology of reproduction, Vol. 79, Issue 3, pp. 450-8, 2008 Method employed by authors: Flow Cytometry (FACS), Flow Cytometry (FACS) (Sample species: Mouse (Murine)).

Allan, Häusler, Wei, Gooi, Quinn, Crimeen-Irwin, Pompolo, Sims, Gillespie, Onyia, Martin: "EphrinB2 regulation by PTH and PTHrP revealed by molecular profiling in differentiating osteoblasts." in: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, Vol. 23, Issue 8, pp. 1170-81, 2008 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Rat (Rattus)).

Yang, Pasquale, Owen, Ethell: "The EphB4 receptor-tyrosine kinase promotes the migration of melanoma cells through Rho-mediated actin cytoskeleton reorganization." in: The Journal of biological chemistry, Vol. 281, Issue 43, pp. 32574-86, 2006 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Mouse (Murine)).

Füller, Korff, Kilian, Dandekar, Augustin: "Forward EphB4 signaling in endothelial cells controls cellular repulsion and segregation from ephrinB2 positive cells." in: Journal of cell science, Vol. 116, Issue Pt 12, pp. 2461-70, 2003 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Images

Product Details anti-Ephrin B2 Antibody Target Details Ephrin B2 Application Details Handling References for anti-Ephrin B2 antibody (ABIN4900008) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Ephrin B2 (EFNB2) (AA 27-227) antibody (ABIN4900008) Detection of Ephrin-B2 in SH-SY5Y Human Cell Line by Flow Cytometry. SH-SY5Y human ne...
Immunocytochemistry (ICC) image for anti-Ephrin B2 (EFNB2) (AA 27-227) antibody (ABIN4900008) Ephrin-B2 in Rat Hippo-campal Neurons. Ephrin-B2 was detected in immersion fixed rat ...
Western Blotting (WB) image for anti-Ephrin B2 (EFNB2) (AA 27-227) antibody (ABIN4900008) Detection of Mouse Ephrin-B2 by Western Blot. Western blot shows lysates of mouse emb...