anti-Human HER2 antibody for Enzyme Immunoassay

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • CD340
  • HER-2
  • HER-2/neu
  • HER2
  • MLN 19
  • NEU
  • NGL
  • TKR1
  • Erbb-2
  • Neu
  • c-erbB2
  • c-neu
  • mKIAA3023
  • wu:fv70f10
  • zgc:63601
  • erb2
  • erb-b2 receptor tyrosine kinase 2
  • ERBB2
  • Erbb2
  • erbb2
Epitope
pSer1113
100
92
72
63
61
55
55
48
46
35
26
22
21
21
20
20
20
20
20
20
20
20
20
19
19
19
19
17
15
15
13
13
12
12
11
8
8
8
8
8
7
6
6
5
5
5
5
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1360
493
321
35
34
15
13
8
6
5
4
3
3
3
2
2
1
1
1
Host
Mouse
993
510
32
14
13
5
3
Clonality
Monoclonal
Conjugate
This HER2 antibody is un-conjugated
76
66
59
56
55
41
19
19
12
12
12
10
10
8
8
8
8
8
7
7
7
7
7
7
7
7
6
3
2
2
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
731
625
326
321
319
313
254
133
111
102
88
21
18
18
8
6
6
5
3
3
3
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN126790
$ 555.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN359842 EIA WB Rabbit Ig Fraction Tyr1248 Log in to see Polyclonal 3
1 ABIN5552237 EIA FACS IHC (p) WB Rabbit Ig Fraction AA 30-59, N-Term Log in to see Polyclonal 0
1 ABIN5554778 EIA IHC (p) WB Chicken IgG C-Term Log in to see Polyclonal 0
1 ABIN5552238 EIA IHC (p) Rabbit Ig Fraction Log in to see Polyclonal 0
1 ABIN492347 EIA IF WB Biotin Mouse IgG1 pSer1113 Log in to see 9-00E-010 0
1 ABIN265463 EIA WB Rabbit Log in to see Polyclonal 0
1 ABIN265465 EIA IHC (p) WB Rabbit Log in to see Polyclonal 0
1 ABIN265464 EIA IHC (p) WB Rabbit Log in to see Polyclonal 0
1 ABIN318038 EIA IHC (p) IP WB Rabbit pTyr877 Log in to see Polyclonal 0
1 ABIN782315 EIA IF IHC (p) WB Mouse IgG2b Log in to see 4-00E-001 0
1 ABIN115506 EIA WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN1106312 EIA FACS IHC (p) WB Mouse IgG1 Log in to see 6C2 2
1 ABIN5541742 EIA IHC (p) Goat Internal Region Log in to see Polyclonal 0
1 ABIN5541743 EIA IHC (p) Goat AA 182-194, Internal Region Log in to see Polyclonal 0
1 ABIN1106311 EIA IHC (p) WB Mouse Log in to see 6C2B12 2
1 ABIN121528 EIA IHC (fro) WB Rabbit pThr686 Log in to see Polyclonal 0
1 ABIN641228 CA DB EIA IA ISt ELISA WB Rabbit IgG pTyr877 Log in to see Polyclonal 0
1 ABIN2717975 EIA ICC IF IHC (p) WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Epitope pSer1113
(100), (92), (72), (63), (61), (55), (55), (48), (46), (35), (26), (22), (21), (21), (20), (20), (20), (20), (20), (20), (20), (20), (20), (19), (19), (19), (19), (17), (15), (15), (13), (13), (12), (12), (11), (8), (8), (8), (8), (8), (7), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1360), (493), (321), (35), (34), (15), (13), (8), (6), (5), (4), (3), (3), (3), (2), (2), (1), (1), (1)
Host Mouse
(993), (510), (32), (14), (13), (5), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HER2 antibody is un-conjugated
(76), (66), (59), (56), (55), (41), (19), (19), (12), (12), (12), (10), (10), (8), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (6), (3), (2), (2)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(731), (625), (326), (321), (319), (313), (254), (133), (111), (102), (88), (21), (18), (18), (8), (6), (6), (5), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling Images
Specificity This antibody specifically recognizes erbB2 phosphorylated at Serine 1113 at 185 kDa.
Characteristics Synonyms: HER-2, NEU, p185erbB2, NGL, c-erbB-2, MNL19, Receptor tyrosine-protein kinase erbB-2,NEU proto-oncogene, Tyrosine kinase-type cell surface receptor HER2
Purification Subsequent Thiophilic and Size Exclusion Chromatography.
Components incl. positive Control
Immunogen Phosphopetide conjugated to KLH. Epitope: Phospho-Serine 1113AA Sequence: Q R Y pS E D P
Clone 9-00E-010
Isotype IgG1

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD340 / ERBB2 / HER2 (ERBB2 Antibody Abstract)
Background ErbB2 is a member of the EGFR/erbB-receptor tyrosine kinase family. ErbB2 is activated upon ligand dependent heterodimerization with EGFR or erbB4. ErbB2 homodimers are not favored due to the lack of an erbB2 specific extracellular ligand. Heterodimerization with EGFR or erbB4 leads to activation of the intrinsic tyrosine kinase activity of EGFR or erbB4 resulting in phosphorylation of multiple tyrosine residues within the erbB2 intracellular domain: Tyr 1023, Tyr 1112, Tyr 1139, Tyr 1196, Tyr 1222, and Tyr 1248. Transphosphorylation via src family kinases leads to phosphorylation of Tyr 877, via PKC of Thr 686, via CamKinase2 of Ser 1113. Phosphorylation of erbB2 at serine 1113 has been correlated with erbB2 overexpression and a poor prognosis. Thus the phosphorylation status of serine 1113 in erbB2 represents a novel and informative biomarker of cancer cell biology and tumor behavior. (Mol. Cell Biochem. 218: 47-54, 2001).Synonyms: HER-2, MNL19, NEU, NEU proto-oncogene, NGL, Receptor tyrosine-protein kinase erbB-2, Tyrosine kinase-type cell surface receptor HER2, c-erbB-2, p185erbB2
Gene ID 2064
UniProt P04626
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling Images back to top
Application Notes ELISA: Use at 0.1 μg/mLImmunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Immunocytochemistry: Use 1-10 μg/mLIncluded Positive Control: Cell lysate from untreated pervanadate-treated A431 cells (SeeProtocols for more details).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol Positive Control Provided. Cell lysate from A 431 pervanadate treatedDescription: Cell lysate from pervanadate-treated A431 cells, epidermoid carcinoma(Human)Format: Lyophilized cell lysate from serum starved A431 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.
Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details Images back to top
Reconstitution Restore with 1 mL H2O (15 min, RT).
Buffer 1 mLPBS containing 0.09 % Sodium Azide/PEG and Sucrose.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C/-80 °C
Storage Comment Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.
Expiry Date 12 months

Images

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pSer1113) antibody (ABIN126790) anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pSer1113) antibody