anti-Human HER2 antibody for Immunocytochemistry

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • CD340
  • HER-2
  • HER-2/neu
  • HER2
  • MLN 19
  • NEU
  • NGL
  • TKR1
  • Erbb-2
  • Neu
  • c-erbB2
  • c-neu
  • mKIAA3023
  • wu:fv70f10
  • zgc:63601
  • erb2
  • erb-b2 receptor tyrosine kinase 2
  • ERBB2
  • Erbb2
  • erbb2
Epitope
AA 23-652
130
87
71
69
60
48
48
35
31
24
24
22
22
15
14
13
13
13
12
12
12
11
11
11
11
10
10
10
10
10
10
9
9
8
7
7
7
6
6
6
6
5
5
5
5
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1294
405
313
31
22
10
6
6
6
4
4
3
3
3
3
3
2
2
2
2
1
1
1
Host
Mouse
773
583
23
16
10
3
3
Clonality
Monoclonal
Conjugate
This HER2 antibody is un-conjugated
57
44
44
42
41
27
18
18
11
11
11
10
10
9
8
8
8
8
8
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
2
2
1
Application
Cytometry by Time of Flight (CyTOF), ELISA (Capture), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)
671
453
411
411
351
192
192
102
100
99
95
47
24
21
18
10
6
3
3
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN4899767
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4309125 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 2
1 ABIN4309121 ICC IF IHC IHC (p) WB Rabbit pTyr1248 Log in to see Polyclonal 2
1 ABIN4309119 ICC IF IHC IHC (p) WB Rabbit pTyr877 Log in to see Polyclonal 1
1 ABIN5945708 ICC IF IHC IHC (p) IP WB Rabbit IgG C-Term Log in to see SI16-01 0
1 ABIN295912 ICC IHC IHC (p) WB Chicken IgY AA 153-433 Log in to see Polyclonal 0
1 ABIN4309122 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal 0
1 ABIN4309198 ELISA FACS ICC IF IHC (fro) Alexa Fluor 488 Mouse IgG1 kappa Log in to see HRB2-282 0
1 ABIN6256054 ELISA ICC IF IHC IP WB Rabbit IgG pTyr877 Log in to see Polyclonal 0
1 ABIN6255774 ELISA ICC IF IHC WB Rabbit IgG pTyr1112 Log in to see Polyclonal 0
1 ABIN6271264 ELISA ICC IF WB Rabbit IgG pTyr1139 Log in to see Polyclonal 0
1 ABIN6256630 ELISA ICC IF IHC WB Rabbit IgG pTyr1248 Log in to see Polyclonal 0
1 ABIN6271262 ELISA ICC IF IHC WB Rabbit IgG pThr686 Log in to see Polyclonal 0
1 ABIN4309126 FACS ICC IF IHC IHC (p) WB Mouse IgG2a Log in to see 4F10 0
1 ABIN2661687 Func ICC FACS IF ICFC IP WB FITC Mouse IgG1 kappa Log in to see 24D2 6
1 ABIN5076576 ICC IF Rabbit IgG Log in to see Polyclonal 0
1 ABIN5076577 ICC IF Rabbit IgG Log in to see Polyclonal 0
1 ABIN6262208 ELISA ICC IF WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN6262206 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN6262207 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2467831 ICC IHC WB Chicken AA 153-433 Log in to see Polyclonal 0

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Epitope AA 23-652
(130), (87), (71), (69), (60), (48), (48), (35), (31), (24), (24), (22), (22), (15), (14), (13), (13), (13), (12), (12), (12), (11), (11), (11), (11), (10), (10), (10), (10), (10), (10), (9), (9), (8), (7), (7), (7), (6), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1294), (405), (313), (31), (22), (10), (6), (6), (6), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Host Mouse
(773), (583), (23), (16), (10), (3), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HER2 antibody is un-conjugated
(57), (44), (44), (42), (41), (27), (18), (18), (11), (11), (11), (10), (10), (9), (8), (8), (8), (8), (8), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (2), (2), (1)
Application Cytometry by Time of Flight (CyTOF), ELISA (Capture), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)
(671), (453), (411), (411), (351), (192), (192), (102), (100), (99), (95), (47), (24), (21), (18), (10), (6), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling References for anti-HER2 antibody (ABIN4899767) Images
Specificity Detects human ErbB2/Her2 in ELISAs and Western blots. In ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) EGF R, rhErbB3, or rhErbB4.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Immunogen Mouse myeloma cell line NS0-derived recombinant human ErbB2/Her2, Thr23-Thr652, Accession # P04626
Clone 191924
Isotype IgG2b
Plasmids, Primers & others

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling References for anti-HER2 antibody (ABIN4899767) Images back to top
Antigen
Alternative Name ErbB2/Her2 (ERBB2 Antibody Abstract)
Background Alternative Names: CD340, CD340 antigen, c-erb B2/neu protein, EC 2.7.10, EGFR2, HER2, HER-2, HER2EC 2.7.10.1, herstatin, Metastatic lymph node gene 19 protein, MLN 19, MLN19, Neu Oncogene, NEUHER-2/neu, neuroblastoma/glioblastoma derived oncogene homolog, NGL, NGLTKR1, p185erbB2, Proto-oncogene c-ErbB-2, Proto-oncogene Neu, receptor tyrosine-protein kinase erbB-2, TKR1, Tyrosine kinase-type cell surface receptor HER2, v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog), v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)
Gene ID 2064
UniProt P04626
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling References for anti-HER2 antibody (ABIN4899767) Images back to top
Application Notes Western Blot 1 μg/mL
Flow Cytometry 0.25 μg/10^6 cells
Immunohistochemistry 8-25 μg/mL
Immunocytochemistry 8-25 μg/mL
CyTOF-ready
ELISA Capture (Matched Antibody Pair) 2-8 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details References for anti-HER2 antibody (ABIN4899767) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-HER2 antibody (ABIN4899767)

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling Images back to top
Product cited in:

Day, Hiles, Kozminsky, Dawsey, Paul, Broses, Shah, Kunja, Hall, Palanisamy, Daignault-Newton, El-Sawy, Wilson, Chou, Ignatoski, Keller, Thomas, Nagrath, Morgan, Day: "HER2 and EGFR Overexpression Support Metastatic Progression of Prostate Cancer to Bone." in: Cancer research, Vol. 77, Issue 1, pp. 74-85, 2016 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Khan, Zhao, Ghosh, Ziman, Sweeney, Kung, Luciw: "Microbead arrays for the analysis of ErbB receptor tyrosine kinase activation and dimerization in breast cancer cells." in: Assay and drug development technologies, Vol. 8, Issue 1, pp. 27-36, 2010 Method employed by authors: Immunoprecipitation (IP) (Sample species: Human).

Images

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling References for anti-HER2 antibody (ABIN4899767) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 23-652) antibody (ABIN4899767) Detection of ErbB2/Her2 in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breas...
Immunocytochemistry (ICC) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 23-652) antibody (ABIN4899767) ErbB2/Her2 in MDA-MB-231 Human Cell Line. ErbB2/Her2 was detected in immersion fixed ...
Immunohistochemistry (IHC) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 23-652) antibody (ABIN4899767) ErbB2/Her2 in Human Breast Cancer Tissue. ErbB2/Her2 was detected in immersion fixed ...
Immunohistochemistry (IHC) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 23-652) antibody (ABIN4899767) ErbB2/Her2 in Human Stomach. ErbB2/Her2 was detected in immersion fixed paraffin-embe...