anti-Human HER2 antibody for Immunocytochemistry

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • CD340
  • HER-2
  • HER-2/neu
  • HER2
  • MLN 19
  • NEU
  • NGL
  • TKR1
  • Erbb-2
  • Neu
  • c-erbB2
  • c-neu
  • mKIAA3023
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog
  • v-erb-b2 avian erythroblastic leukemia viral oncogene 2
  • v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
  • ERBB2
  • Erbb2
Epitope
AA 23-652
101
95
72
69
61
57
55
55
48
35
26
22
22
21
21
20
20
20
20
20
20
20
20
20
19
19
19
17
17
15
15
13
13
12
11
8
8
8
8
8
7
7
6
6
5
5
5
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1386
502
325
36
34
15
13
8
6
5
4
3
3
3
2
1
1
1
1
1
Host
Mouse
1007
522
38
16
13
5
1
Clonality
Monoclonal
Conjugate
This HER2 antibody is un-conjugated
76
66
59
55
47
41
21
21
14
14
13
12
12
9
8
8
8
8
8
7
7
7
7
7
7
7
7
6
3
2
2
Application
Cytometry by Time of Flight (CyTOF), ELISA (Capture), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)
720
602
333
320
309
309
269
134
112
102
90
21
21
19
8
6
6
5
4
3
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN4899768
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
26.561703 ABIN4899767 CyTOF ELISA (Capture) FACS ICC IHC WB Mouse IgG2b AA 23-652 Log in to see 191924 2
1 ABIN4309121 ICC IF IHC IHC (p) WB Rabbit pTyr1248 Log in to see Polyclonal 2
1 ABIN4309125 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 2
1 ABIN4309119 ICC IF IHC IHC (p) WB Rabbit pTyr877 Log in to see Polyclonal
1 ABIN295912 ICC IHC (p) WB Chicken IgY AA 153-433 Log in to see Polyclonal
1 ABIN4309122 ICC IF IHC IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN2661687 Func ICC FACS IF ICFC IP WB FITC Mouse IgG1 kappa Log in to see 24D2 6
1 ABIN4309126 FACS ICC IF IHC IHC (p) WB Mouse IgG2a Log in to see 4F10
1 ABIN5076576 ICC IF Rabbit IgG Log in to see Polyclonal
1 ABIN5076577 ICC IF Rabbit IgG Log in to see Polyclonal
1 ABIN2467831 ICC IHC WB Chicken AA 153-433 Log in to see Polyclonal
1 ABIN2469599 ICC IHC WB Chicken IgY AA 153-433 Log in to see Polyclonal
1 ABIN4309148 FACS ICC IF Mouse IgG1 kappa Log in to see HRB2-282
1 ABIN4309149 FACS ICC IF Mouse IgG1 kappa Log in to see HRB2-282
1 ABIN4309150 FACS ICC IF Mouse IgG1 kappa Log in to see HRB2-282
1 ABIN4309152 FACS ICC IF Mouse IgG1 kappa Log in to see HRB2-718
1 ABIN4309151 FACS ICC IF Mouse IgG1 kappa Log in to see HRB2-718
1 ABIN4309153 FACS ICC IF Mouse IgG1 kappa Log in to see HRB2-718
1 ABIN4309146 FACS ICC IF Mouse IgG2a kappa Log in to see HRB2-258
1 ABIN4309147 FACS ICC IF Mouse IgG2a kappa Log in to see HRB2-258

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Epitope AA 23-652
(101), (95), (72), (69), (61), (57), (55), (55), (48), (35), (26), (22), (22), (21), (21), (20), (20), (20), (20), (20), (20), (20), (20), (20), (19), (19), (19), (17), (17), (15), (15), (13), (13), (12), (11), (8), (8), (8), (8), (8), (7), (7), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1386), (502), (325), (36), (34), (15), (13), (8), (6), (5), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Host Mouse
(1007), (522), (38), (16), (13), (5), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HER2 antibody is un-conjugated
(76), (66), (59), (55), (47), (41), (21), (21), (14), (14), (13), (12), (12), (9), (8), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (6), (3), (2), (2)
Application Cytometry by Time of Flight (CyTOF), ELISA (Capture), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)
(720), (602), (333), (320), (309), (309), (269), (134), (112), (102), (90), (21), (21), (19), (8), (6), (6), (5), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling References for anti-HER2 antibody (ABIN4899768) Images
Specificity Detects human ErbB2/Her2 in ELISAs and Western blots. In ELISAs and Western blots, this antibody does not cross-react with recombinant human (rh) EGF R, rhErbB3, or rhErbB4.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Immunogen Mouse myeloma cell line NS0-derived recombinant human ErbB2/Her2, Thr23-Thr652, Accession # P04626
Clone 191924
Isotype IgG2b

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling References for anti-HER2 antibody (ABIN4899768) Images back to top
Antigen
Alternative Name ErbB2/Her2 (ERBB2 Antibody Abstract)
Background Alternative Names: CD340, CD340 antigen, c-erb B2/neu protein, EC 2.7.10, EGFR2, HER2, HER-2, HER2EC 2.7.10.1, herstatin, Metastatic lymph node gene 19 protein, MLN 19, MLN19, Neu Oncogene, NEUHER-2/neu, neuroblastoma/glioblastoma derived oncogene homolog, NGL, NGLTKR1, p185erbB2, Proto-oncogene c-ErbB-2, Proto-oncogene Neu, receptor tyrosine-protein kinase erbB-2, TKR1, Tyrosine kinase-type cell surface receptor HER2, v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog), v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)
Gene ID 2064
UniProt P04626
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling References for anti-HER2 antibody (ABIN4899768) Images back to top
Application Notes Western Blot 1 μg/mL
Flow Cytometry 0.25 μg/10^6 cells
Immunohistochemistry 8-25 μg/mL
Immunocytochemistry 8-25 μg/mL
CyTOF-ready
ELISA Capture (Matched Antibody Pair) 2-8 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details References for anti-HER2 antibody (ABIN4899768) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-HER2 antibody (ABIN4899768)

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling Images back to top
Product cited in:

Day, Hiles, Kozminsky, Dawsey, Paul, Broses, Shah, Kunja, Hall, Palanisamy, Daignault-Newton, El-Sawy, Wilson, Chou, Ignatoski, Keller, Thomas, Nagrath, Morgan, Day: "HER2 and EGFR Overexpression Support Metastatic Progression of Prostate Cancer to Bone." in: Cancer research, Vol. 77, Issue 1, pp. 74-85, 2016 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Khan, Zhao, Ghosh, Ziman, Sweeney, Kung, Luciw: "Microbead arrays for the analysis of ErbB receptor tyrosine kinase activation and dimerization in breast cancer cells." in: Assay and drug development technologies, Vol. 8, Issue 1, pp. 27-36, 2010 Method employed by authors: Immunoprecipitation (IP) (Sample species: Human).

Images

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling References for anti-HER2 antibody (ABIN4899768) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 23-652) antibody (ABIN4899768) Detection of ErbB2/Her2 in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breas...
Immunocytochemistry (ICC) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 23-652) antibody (ABIN4899768) ErbB2/Her2 in MDA-MB-231 Human Cell Line. ErbB2/Her2 was detected in immersion fixed ...
Immunohistochemistry (IHC) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 23-652) antibody (ABIN4899768) ErbB2/Her2 in Human Breast Cancer Tissue. ErbB2/Her2 was detected in immersion fixed ...
Immunohistochemistry (IHC) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 23-652) antibody (ABIN4899768) ErbB2/Her2 in Human Stomach. ErbB2/Her2 was detected in immersion fixed paraffin-embe...
Flow Cytometry (FACS) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 23-652) antibody (ABIN4899768) Detection of ErbB2/Her2 in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breas...
Immunohistochemistry (IHC) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 23-652) antibody (ABIN4899768) ErbB2/Her2 in Human Breast Cancer Tissue. ErbB2/Her2 was detected in immersion fixed ...
Immunohistochemistry (IHC) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 23-652) antibody (ABIN4899768) ErbB2/Her2 in Human Stomach. ErbB2/Her2 was detected in immersion fixed paraffin-embe...
Immunocytochemistry (ICC) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 23-652) antibody (ABIN4899768) ErbB2/Her2 in MDA-MB-231 Human Cell Line. ErbB2/Her2 was detected in immersion fixed ...