anti-Human HER2 antibody for Immunofluorescence

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • CD340
  • HER-2
  • HER-2/neu
  • HER2
  • MLN 19
  • NEU
  • NGL
  • TKR1
  • Erbb-2
  • Neu
  • c-erbB2
  • c-neu
  • mKIAA3023
  • wu:fv70f10
  • zgc:63601
  • erb2
  • erb-b2 receptor tyrosine kinase 2
  • ERBB2
  • Erbb2
  • erbb2
Epitope
AA 182-373
85
63
63
54
46
44
41
31
27
27
26
22
19
18
15
15
14
13
12
11
11
11
11
11
11
11
11
11
11
11
11
10
10
8
8
7
7
7
7
7
6
6
5
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1097
379
256
28
21
8
4
4
4
4
3
3
3
2
1
1
1
Host
Mouse
730
438
32
12
11
3
3
Clonality
Monoclonal
Conjugate
This HER2 antibody is un-conjugated
53
45
38
37
36
21
19
19
12
12
12
10
10
8
8
8
8
8
7
7
7
7
7
7
7
7
6
3
2
2
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
579
433
290
278
266
205
177
102
102
101
86
21
18
18
8
6
5
4
3
3
3
2
1
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN967787
Contact our Customer Service for availability and price in your country.
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.747924 ABIN1532607 IF ELISA Rabbit IgG AA 661-710 Log in to see Polyclonal 3
11.747924 ABIN1531590 IF ELISA Rabbit IgG AA 661-710, pThr686 Log in to see Polyclonal 3
11.747924 ABIN4309121 ICC IF IHC IHC (p) WB Rabbit pTyr1248 Log in to see Polyclonal 2
11.747924 ABIN4309119 ICC IF IHC IHC (p) WB Rabbit pTyr877 Log in to see Polyclonal 1
11.747924 ABIN2957598 IC IF IHC WB Rabbit Log in to see Polyclonal 0
11.747924 ABIN1534423 IF IHC ELISA WB Rabbit IgG AA 641-690 Log in to see Polyclonal 1
11.747924 ABIN967786 IF IP IHC WB Mouse IgG2b AA 182-373 Log in to see 42-c-erbB 5
11.747924 ABIN1532624 IF ELISA Rabbit IgG AA 1111-1160 Log in to see Polyclonal 0
11.747924 ABIN4309125 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 2
11.747924 ABIN2454964 FACS IF IHC WB Mouse IgG2a AA 676-1255 Log in to see 4F10 0
11.747924 ABIN2455003 IF IHC WB Mouse IgG2b AA 676-1255 Log in to see 1D9 0
11.747924 ABIN2455009 FACS IF IHC WB Mouse IgG2a AA 676-1255 Log in to see 1C5 0
11.747924 ABIN2455010 IF IHC WB Mouse IgG2a AA 676-1255 Log in to see 11A7 0
11.747924 ABIN2455011 FACS IF IHC WB Mouse IgG2b AA 676-1255 Log in to see 3F11 0
11.747924 ABIN2455012 FACS IF IHC WB Mouse IgG2b AA 676-1255 Log in to see 9G2 0
11.747924 ABIN2455013 IF IHC WB Mouse IgG2a AA 676-1255 Log in to see 12G4 0
11.747924 ABIN1995358 FACS IF IF/ICC PE Rabbit IgG AA 1-652, Extracellular Domain Log in to see 511 0
11.747924 ABIN1870165 IF WB Rabbit IgG pTyr1221, pTyr1222 Log in to see Polyclonal 0
11.747924 ABIN1870167 IF IHC WB Rabbit IgG pTyr1248 Log in to see Polyclonal 0
11.747924 ABIN1539721 DB IF Rabbit Ig pThr1172 Log in to see Polyclonal 0

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Epitope AA 182-373
(85), (63), (63), (54), (46), (44), (41), (31), (27), (27), (26), (22), (19), (18), (15), (15), (14), (13), (12), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (10), (10), (8), (8), (7), (7), (7), (7), (7), (6), (6), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1097), (379), (256), (28), (21), (8), (4), (4), (4), (4), (3), (3), (3), (2), (1), (1), (1)
Host Mouse
(730), (438), (32), (12), (11), (3), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This HER2 antibody is un-conjugated
(53), (45), (38), (37), (36), (21), (19), (19), (12), (12), (12), (10), (10), (8), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (6), (3), (2), (2)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(579), (433), (290), (278), (266), (205), (177), (102), (102), (101), (86), (21), (18), (18), (8), (6), (5), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
Supplier Log in to see

Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling References for anti-HER2 antibody (ABIN967787) Images
Cross-Reactivity Human
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Rat ErbB2 aa. 182-373
Clone 42-c-erbB
Isotype IgG2b

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling References for anti-HER2 antibody (ABIN967787) Images back to top
Antigen
Alternative Name c-erbB-2 (ERBB2 Antibody Abstract)
Background ErbB2 (Neu or Her-2) is a member of the erbB family of growth factor receptors. These factors possess constitutive tyrosine kinase activity and are commonly overexpressed in breast and ovarian carcinomas. While other erbB family members' ligands, such a EGF and NDF, are well characterized, a natural ligand for erbB2 remains unknown. ErbB2 forms heterodimers with erbB1/EGFR, erbB3, and erbB4, and can modulate their ligand affinities. Thus, erbB2 alters the intracellular responses elicited by EGF and NDF. This control is due to the fact that erbB2, when in complex with another erbB family receptor, decelerates the rate of ligand dissociation. Therefore, erbB2 may act as a signaling subunit for other receptors rather than a true growth factor receptor. Due to high sequence homology, this antibody may cross-react with the 180 kDa EGFR.
Synonyms: Neu, Her-2
Molecular Weight 185 kDa
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling References for anti-HER2 antibody (ABIN967787) Images back to top
Comment

Related Products: ABIN968533, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details References for anti-HER2 antibody (ABIN967787) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.

References for anti-HER2 antibody (ABIN967787)

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling Images back to top
Product cited in:

Dillon, Creer, Kerr, Kümin, Dickson: "Basolateral targeting of ERBB2 is dependent on a novel bipartite juxtamembrane sorting signal but independent of the C-terminal ERBIN-binding domain." in: Molecular and cellular biology, Vol. 22, Issue 18, pp. 6553-63, 2002

Piechocki, Pilon, Wei: "Complementary antitumor immunity induced by plasmid DNA encoding secreted and cytoplasmic human ErbB-2." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 6, pp. 3367-74, 2001

Xu, Mimnaugh, Rosser, Nicchitta, Marcu, Yarden, Neckers: "Sensitivity of mature Erbb2 to geldanamycin is conferred by its kinase domain and is mediated by the chaperone protein Hsp90." in: The Journal of biological chemistry, Vol. 276, Issue 5, pp. 3702-8, 2001

Graus-Porta, Beerli, Hynes: "Single-chain antibody-mediated intracellular retention of ErbB-2 impairs Neu differentiation factor and epidermal growth factor signaling." in: Molecular and cellular biology, Vol. 15, Issue 3, pp. 1182-91, 1995

Szöllösi, Balázs, Feuerstein, Benz, Waldman: "ERBB-2 (HER2/neu) gene copy number, p185HER-2 overexpression, and intratumor heterogeneity in human breast cancer." in: Cancer research, Vol. 55, Issue 22, pp. 5400-7, 1995

Images

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling References for anti-HER2 antibody (ABIN967787) back to top
Supplier Images
Western Blotting (WB) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 182-373) antibody (ABIN967787) Western blot analysis ErbB2 on a A431 cell lysate (Human epithelial carcinoma, ATCC C...
Immunofluorescence (IF) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 182-373) antibody (ABIN967787) Immunofluorescence staining of A431 cells (Human epithelial carcinoma, ATCC CRL-1555).
 image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 182-373) antibody (ABIN967787) anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 182-373) antibody (Image 3)
Western Blotting (WB) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 182-373) antibody (ABIN967787) anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 182-373) antibody (Image 4)