anti-Human HER2 antibody for Immunofluorescence

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • CD340
  • HER-2
  • HER-2/neu
  • HER2
  • MLN 19
  • NEU
  • NGL
  • TKR1
  • Erbb-2
  • Neu
  • c-erbB2
  • c-neu
  • mKIAA3023
  • wu:fv70f10
  • zgc:63601
  • erb2
  • erb-b2 receptor tyrosine kinase 2
  • ERBB2
  • Erbb2
  • erbb2
Epitope
AA 661-710, pThr686
128
86
70
68
59
49
48
35
30
30
25
22
20
16
15
15
13
13
12
12
11
11
11
11
11
11
11
11
11
11
11
11
10
8
8
7
7
7
6
6
6
5
5
5
5
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1302
399
296
28
21
8
4
4
4
4
3
3
3
2
1
1
1
Host
Rabbit
786
587
29
14
11
3
3
Clonality
Polyclonal
Conjugate
This HER2 antibody is un-conjugated
59
45
44
42
41
27
20
20
13
13
13
13
11
11
8
8
8
8
8
7
7
7
7
7
7
7
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
3
2
2
1
Application
Immunofluorescence (IF), ELISA
669
465
429
396
342
201
198
102
102
101
87
47
24
21
18
11
6
4
3
3
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN1531590
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.155504 ABIN1532607 IF ELISA Rabbit IgG AA 661-710 Log in to see Polyclonal 3
12.155504 ABIN4309121 ICC IF IHC IHC (p) WB Rabbit pTyr1248 Log in to see Polyclonal 2
12.155504 ABIN4309119 ICC IF IHC IHC (p) WB Rabbit pTyr877 Log in to see Polyclonal 1
12.155504 ABIN2957598 IC IF IHC WB Rabbit Log in to see Polyclonal 0
12.155504 ABIN1534423 IF IHC ELISA WB Rabbit IgG AA 641-690 Log in to see Polyclonal 1
12.155504 ABIN1532624 IF ELISA Rabbit IgG AA 1111-1160 Log in to see Polyclonal 0
12.155504 ABIN4309125 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 2
12.155504 ABIN2717958 FACS IF IHC WB Mouse IgG2b AA 676-1255 Log in to see 3F11 0
12.155504 ABIN2717953 FACS IF IHC WB Mouse IgG2a AA 676-1255 Log in to see 4F10 0
12.155504 ABIN2717954 IF IHC WB Mouse IgG2b AA 676-1255 Log in to see 1D9 0
12.155504 ABIN2717957 IF IHC WB Mouse IgG2a AA 676-1255 Log in to see 11A7 0
12.155504 ABIN2717959 FACS IF IHC WB Mouse IgG2b AA 676-1255 Log in to see 9G2 0
12.155504 ABIN2717960 IF IHC WB Mouse IgG2a AA 676-1255 Log in to see 12G4 0
12.155504 ABIN2717956 FACS IF IHC WB Mouse IgG2a AA 676-1255 Log in to see 1C5 0
12.155504 ABIN1995358 FACS IF IF/ICC PE Rabbit IgG AA 1-652, Extracellular Domain Log in to see 511 0
12.155504 ABIN1870165 IF WB Rabbit IgG pTyr1221, pTyr1222 Log in to see Polyclonal 0
12.155504 ABIN1870167 IF IHC WB Rabbit IgG pTyr1248 Log in to see Polyclonal 0
12.155504 ABIN1539721 DB IF Rabbit Ig pThr1172 Log in to see Polyclonal 0
12.155504 ABIN1995350 FACS IF IF/ICC FITC Mouse IgG1 AA 1-652 Log in to see 07 0
12.155504 ABIN515370 IF IHC (p) PLA ELISA WB Mouse IgG2b kappa AA 22-121, partial Log in to see 3B1 0

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Epitope AA 661-710, pThr686
(128), (86), (70), (68), (59), (49), (48), (35), (30), (30), (25), (22), (20), (16), (15), (15), (13), (13), (12), (12), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (10), (8), (8), (7), (7), (7), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1302), (399), (296), (28), (21), (8), (4), (4), (4), (4), (3), (3), (3), (2), (1), (1), (1)
Host Rabbit
(786), (587), (29), (14), (11), (3), (3)
Clonality
Polyclonal
Conjugate This HER2 antibody is un-conjugated
(59), (45), (44), (42), (41), (27), (20), (20), (13), (13), (13), (13), (11), (11), (8), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (3), (2), (2), (1)
Application Immunofluorescence (IF), ELISA
(669), (465), (429), (396), (342), (201), (198), (102), (102), (101), (87), (47), (24), (21), (18), (11), (6), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling References for anti-HER2 antibody (ABIN1531590) Images
Specificity

HER2 (Phospho-Thr686) Antibody detects endogenous levels of HER2 only when phosphorylated at Thr686.

PhosphorylationH:T686 M:T687 R:T688

Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human HER2 around the phosphorylation site of Thr686.
Isotype IgG
Plasmids, Primers & others

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling References for anti-HER2 antibody (ABIN1531590) Images back to top
Antigen
Alternative Name HER2 (ERBB2 Antibody Abstract)
Background

Synonyms: C-erbB-2, Epidermal growth factor receptor-related protein, ErbB2, HER2, MLN 19, NEU, NEU proto-oncogene, NGL, Receptor protein-tyrosine kinase erbB-2, Receptor protein-tyrosine kinase erbB-2 precursor, Tyrosine kinase-type cell surface receptor HER2, kin

NCBI Gene Symbol: ERBB2

Molecular Weight 137 kDa
Gene ID 2064
OMIM 164870
UniProt P04626
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling References for anti-HER2 antibody (ABIN1531590) Images back to top
Application Notes IF: 1:100~1:500 ELISA: 1:5000
Comment

Unigene-Number: Hs.446352 (NCBI Gene Symbol: ERBB2)

Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details References for anti-HER2 antibody (ABIN1531590) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20°C for at least 1 year.
Expiry Date 12 months

References for anti-HER2 antibody (ABIN1531590)

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling Images back to top
Background publications

Olsen, Blagoev, Gnad, Macek, Kumar, Mortensen, Mann: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006

Yamamoto, Ikawa, Akiyama, Semba, Nomura, Miyajima, Saito, Toyoshima: "Similarity of protein encoded by the human c-erb-B-2 gene to epidermal growth factor receptor." in: Nature, Vol. 319, Issue 6050, pp. 230-4, 1986

King, Kraus, Aaronson: "Amplification of a novel v-erbB-related gene in a human mammary carcinoma." in: Science (New York, N.Y.), Vol. 229, Issue 4717, pp. 974-6, 1985

Images

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling References for anti-HER2 antibody (ABIN1531590) back to top
Supplier Images
Immunofluorescence (IF) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 661-710), (pThr686) antibody (ABIN1531590) Immunofluorescence analysis of HuvEc cells, using HER2 (Phospho-Thr686) Antibody. The...