anti-Human HER2 antibody for Immunohistochemistry

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • CD340
  • HER-2
  • HER-2/neu
  • HER2
  • MLN 19
  • NEU
  • NGL
  • TKR1
  • Erbb-2
  • Neu
  • c-erbB2
  • c-neu
  • mKIAA3023
  • wu:fv70f10
  • zgc:63601
  • erb2
  • erb-b2 receptor tyrosine kinase 2
  • ERBB2
  • Erbb2
  • erbb2
Epitope
AA 23-652
100
92
72
63
61
55
55
48
45
35
26
22
21
21
20
20
20
20
20
20
20
20
20
19
19
19
19
17
15
15
13
13
12
12
11
8
8
8
8
8
7
6
6
6
5
5
5
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1361
494
322
35
34
15
13
8
6
5
4
3
3
3
2
2
1
1
1
Host
Goat
993
511
32
14
13
5
3
Clonality
Polyclonal
Conjugate
This HER2 antibody is un-conjugated
76
66
59
56
55
41
19
19
12
12
12
10
10
8
8
8
8
8
7
7
7
7
7
7
7
7
6
3
2
2
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
732
625
325
320
319
314
255
133
111
102
89
21
19
17
8
6
6
5
3
3
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN4899769
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.0975895 ABIN2704878 IP IHC WB Rabbit C-Term, pTyr1221, pTyr1222 Log in to see Polyclonal 0
9.0975895 ABIN2704879 IP IHC WB Rabbit C-Term Log in to see Polyclonal 0
9.0975895 ABIN1532623 IHC ELISA WB Rabbit IgG AA 661-710 Log in to see Polyclonal 2
9.0975895 ABIN4309120 IHC IHC (p) IP WB Rabbit pTyr877 Log in to see Polyclonal 1
9.0975895 ABIN4309119 ICC IF IHC IHC (p) WB Rabbit pTyr877 Log in to see Polyclonal 1
9.0975895 ABIN4899767 CyTOF ELISA (Capture) FACS ICC IHC WB Mouse IgG2b AA 23-652 Log in to see 191924 2
9.0975895 ABIN4899768 CyTOF ELISA (Capture) FACS ICC IHC WB Mouse IgG2b AA 23-652 Log in to see 191924 2
9.0975895 ABIN2957599 IHC WB Rabbit Log in to see Polyclonal 0
9.0975895 ABIN2957598 IC IF IHC WB Rabbit Log in to see Polyclonal 0
9.0975895 ABIN1534423 IF IHC ELISA WB Rabbit IgG AA 641-690 Log in to see Polyclonal 1
9.0975895 ABIN1532891 IHC ELISA WB Rabbit IgG AA 1206-1255 Log in to see Polyclonal 0
9.0975895 ABIN1532890 IHC ELISA WB Rabbit IgG AA 1191-1240 Log in to see Polyclonal 0
9.0975895 ABIN1532892 IHC ELISA WB Rabbit IgG AA 851-900 Log in to see Polyclonal 0
9.0975895 ABIN1531859 ELISA IHC IP WB Rabbit IgG AA 1191-1240, pTyr1221 Log in to see Polyclonal 0
9.0975895 ABIN1531860 IHC ELISA WB Rabbit IgG AA 1206-1255, pTyr1248 Log in to see Polyclonal 0
9.0975895 ABIN1531861 ELISA IHC IP WB Rabbit IgG AA 851-900, pTyr877 Log in to see Polyclonal 0
9.0975895 ABIN2454964 FACS IF IHC WB Mouse IgG2a AA 676-1255 Log in to see 4F10 0
9.0975895 ABIN2455003 IF IHC WB Mouse IgG2b AA 676-1255 Log in to see 1D9 0
9.0975895 ABIN2455009 FACS IF IHC WB Mouse IgG2a AA 676-1255 Log in to see 1C5 0
9.0975895 ABIN2455010 IF IHC WB Mouse IgG2a AA 676-1255 Log in to see 11A7 0

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Epitope AA 23-652
(100), (92), (72), (63), (61), (55), (55), (48), (45), (35), (26), (22), (21), (21), (20), (20), (20), (20), (20), (20), (20), (20), (20), (19), (19), (19), (19), (17), (15), (15), (13), (13), (12), (12), (11), (8), (8), (8), (8), (8), (7), (6), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1361), (494), (322), (35), (34), (15), (13), (8), (6), (5), (4), (3), (3), (3), (2), (2), (1), (1), (1)
Host Goat
(993), (511), (32), (14), (13), (5), (3)
Clonality
Polyclonal
Conjugate This HER2 antibody is un-conjugated
(76), (66), (59), (56), (55), (41), (19), (19), (12), (12), (12), (10), (10), (8), (8), (8), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (6), (3), (2), (2)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
(732), (625), (325), (320), (319), (314), (255), (133), (111), (102), (89), (21), (19), (17), (8), (6), (6), (5), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling References for anti-HER2 antibody (ABIN4899769) Images
Specificity Detects human ErbB2/Her2 in direct ELISAs and Western blots. In direct ELISAs, less than 20 % cross-reactivity with recombinant mouse ErbB2 is observed and less than 5 % cross-reactivity with recombinant human (rh) ErbB3, rhErbB4, and rhEGF R is observed.
Purification Antigen Affinity-purified
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen Mouse myeloma cell line NS0-derived recombinant human ErbB2/Her2, Thr23-Thr652, Accession # P04626
Isotype IgG

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling References for anti-HER2 antibody (ABIN4899769) Images back to top
Antigen
Alternative Name ErbB2/Her2 (ERBB2 Antibody Abstract)
Background Alternative Names: CD340, CD340 antigen, c-erb B2/neu protein, EC 2.7.10, EGFR2, HER2, HER-2, HER2EC 2.7.10.1, herstatin, Metastatic lymph node gene 19 protein, MLN 19, MLN19, Neu Oncogene, NEUHER-2/neu, neuroblastoma/glioblastoma derived oncogene homolog, NGL, NGLTKR1, p185erbB2, Proto-oncogene c-ErbB-2, Proto-oncogene Neu, receptor tyrosine-protein kinase erbB-2, TKR1, Tyrosine kinase-type cell surface receptor HER2, v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog), v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)
Gene ID 2064
UniProt P04626
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling References for anti-HER2 antibody (ABIN4899769) Images back to top
Application Notes Western Blot 0.1 μg/mL
Flow Cytometry 2.5 μg/10^6 cells
Immunohistochemistry 5-15 μg/mL
Inhibition of Cell Growth 0.06-0.3 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details References for anti-HER2 antibody (ABIN4899769) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-HER2 antibody (ABIN4899769)

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling Images back to top
Product cited in:

Denisova, Jenkins, Evelegh, Bramson: "Novel method for differentiation between Trastuzumab and host adaptive response." in: Molecular immunology, Vol. 48, Issue 15-16, pp. 1882-5, 2011 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Sarup, Jin, Turin, Bai, Beryt, Brdlik, Higaki, Jorgensen, Lau, Lindley, Liu, Ni, Rozzelle, Kumari, Watson, Zhang, Shepard: "Human epidermal growth factor receptor (HER-1:HER-3) Fc-mediated heterodimer has broad antiproliferative activity in vitro and in human tumor xenografts." in: Molecular cancer therapeutics, Vol. 7, Issue 10, pp. 3223-36, 2008 Method employed by authors: ELISA (ELISA), ELISA (ELISA) (Sample species: Human).

Kunii, Davis, Gorenstein, Hatch, Yashiro, Di Bacco, Elbi, Lutterbach: "FGFR2-amplified gastric cancer cell lines require FGFR2 and Erbb3 signaling for growth and survival." in: Cancer research, Vol. 68, Issue 7, pp. 2340-8, 2008 Method employed by authors: Immunoprecipitation (IP), Immunoprecipitation (IP) (Sample species: Human).

Images

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling References for anti-HER2 antibody (ABIN4899769) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (AA 23-652) antibody (ABIN4899769) ErbB2/Her2 in Human Breast Cancer Tissue. ErbB2/Her2 was detected in immersion fixed ...