anti-Mouse (Murine) HER2 antibody for Western Blotting

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • CD340
  • HER-2
  • HER-2/neu
  • HER2
  • MLN 19
  • NEU
  • NGL
  • TKR1
  • Erbb-2
  • Neu
  • c-erbB2
  • c-neu
  • mKIAA3023
  • wu:fv70f10
  • zgc:63601
  • erb2
  • erb-b2 receptor tyrosine kinase 2
  • ERBB2
  • Erbb2
  • erbb2
Epitope
pTyr1248
130
87
71
68
60
48
48
35
31
25
24
22
22
15
14
13
13
13
12
12
12
11
11
11
11
10
10
10
10
10
10
9
9
8
7
7
7
6
6
6
6
5
5
5
5
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1294
404
312
31
22
10
6
6
6
4
4
3
3
3
3
3
2
2
2
2
1
1
1
Host
Rabbit
772
584
23
16
10
3
3
Clonality
Polyclonal
Conjugate
This HER2 antibody is un-conjugated
57
44
44
42
41
27
18
18
11
11
11
10
10
9
8
8
8
8
8
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
2
2
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
671
453
412
410
351
192
191
102
100
99
95
47
24
21
18
11
6
3
3
3
2
1
1
1
1
1
1
1
1
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Available images

Catalog No. ABIN4309121
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1084251 ABIN2704879 IP IHC WB Rabbit C-Term Log in to see Polyclonal 0
3.1084251 ABIN1532014 ELISA WB Rabbit IgG AA 1081-1130, pTyr1112 Log in to see Polyclonal 2
3.1084251 ABIN1532623 IHC ELISA WB Rabbit IgG AA 661-710 Log in to see Polyclonal 2
3.1084251 ABIN1533053 ELISA WB Rabbit IgG AA 1081-1130 Log in to see Polyclonal 2
3.1084251 ABIN4309119 ICC IF IHC IHC (p) WB Rabbit pTyr877 Log in to see Polyclonal 1
3.1084251 ABIN2957599 IHC WB Rabbit Log in to see Polyclonal 0
3.1084251 ABIN2957598 IC IF IHC WB Rabbit Log in to see Polyclonal 0
3.1084251 ABIN1534423 IF IHC ELISA WB Rabbit IgG AA 641-690 Log in to see Polyclonal 1
3.1084251 ABIN1531861 ELISA IHC IP WB Rabbit IgG AA 851-900, pTyr877 Log in to see Polyclonal 0
3.1084251 ABIN1531859 ELISA IHC IP WB Rabbit IgG AA 1191-1240, pTyr1221 Log in to see Polyclonal 0
3.1084251 ABIN1532890 IHC ELISA WB Rabbit IgG AA 1191-1240 Log in to see Polyclonal 0
3.1084251 ABIN1532891 IHC ELISA WB Rabbit IgG AA 1206-1255 Log in to see Polyclonal 0
3.1084251 ABIN1532892 IHC ELISA WB Rabbit IgG AA 851-900 Log in to see Polyclonal 0
3.1084251 ABIN1531860 IHC ELISA WB Rabbit IgG AA 1206-1255, pTyr1248 Log in to see Polyclonal 0
3.1084251 ABIN126788 WB Mouse IgG1 AA 860-880 Log in to see 24B5 0
3.1084251 ABIN126791 WB Mouse IgG1 AA 1240-1260 Log in to see 19D2 0
3.1084251 ABIN126789 IF WB Mouse IgG1 pTyr1112 Log in to see 19G5 0
3.1084251 ABIN126787 WB Mouse IgG1 pThr686 Log in to see 7F8 0
3.1084251 ABIN1870163 IF IHC WB Rabbit IgG pTyr877 Log in to see Polyclonal 0
3.1084251 ABIN1870165 IF WB Rabbit IgG pTyr1221, pTyr1222 Log in to see Polyclonal 0

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Epitope pTyr1248
(130), (87), (71), (68), (60), (48), (48), (35), (31), (25), (24), (22), (22), (15), (14), (13), (13), (13), (12), (12), (12), (11), (11), (11), (11), (10), (10), (10), (10), (10), (10), (9), (9), (8), (7), (7), (7), (6), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1294), (404), (312), (31), (22), (10), (6), (6), (6), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(772), (584), (23), (16), (10), (3), (3)
Clonality
Polyclonal
Conjugate This HER2 antibody is un-conjugated
(57), (44), (44), (42), (41), (27), (18), (18), (11), (11), (11), (10), (10), (9), (8), (8), (8), (8), (8), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (2), (2), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(671), (453), (412), (410), (351), (192), (191), (102), (100), (99), (95), (47), (24), (21), (18), (11), (6), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling References for anti-HER2 antibody (ABIN4309121) Images
Specificity HER2 (specific to Phospho-Tyr1248) detects endogenous levels of HER2 only when phosphorylated at tyrosine1248.
Purification Immunogen affinity purified
Immunogen The antiserum was produced against synthesized phosphopeptide derived from human HER2 around the phosphorylation site of tyrosine1248 (P-E-YP-L-G).
Plasmids, Primers & others

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling References for anti-HER2 antibody (ABIN4309121) Images back to top
Antigen
Alternative Name ErbB2/Her2 (ERBB2 Antibody Abstract)
Background Gene Symbol: ERBB2
Molecular Weight Theoretical MW: 185 kDa
Gene ID 2064
UniProt P04626
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling References for anti-HER2 antibody (ABIN4309121) Images back to top
Application Notes Western Blot 1:500-1:1000, Immunohistochemistry 1:50-1:100, Immunocytochemistry/Immunofluorescence 1:100-1:200, Immunohistochemistry-Paraffin 1:50-1:100The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details References for anti-HER2 antibody (ABIN4309121) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, and 50 % glycerol.
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-HER2 antibody (ABIN4309121)

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling Images back to top
Product cited in:

Scrima, Zito Marino, Oliveira, Marinaro, La Mantia, Rocco, De Marco, Malanga, De Rosa, Rizzuto, Botti, Franco, Zoppoli, Viglietto: "Aberrant Signaling through the HER2-ERK1/2 Pathway is Predictive of Reduced Disease-Free and Overall Survival in Early Stage Non-Small Cell Lung Cancer (NSCLC) Patients." in: Journal of Cancer, Vol. 8, Issue 2, pp. 227-239, 2017

Eden, Ju, Murugesan, Phoenix, Nimmervoll, Tong, Ellison, Finkelstein, Wright, Boulos, Dapper, Thiruvenkatam, Lessman, Taylor, Gilbertson: "Orthotopic models of pediatric brain tumors in zebrafish." in: Oncogene, Vol. 34, Issue 13, pp. 1736-42, 2015 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Images

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling References for anti-HER2 antibody (ABIN4309121) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr1248) antibody (ABIN4309121) Immunohistochemistry-Paraffin: ErbB2/Her2 [p Tyr1248] Antibody [ABIN43091210] - Human...
Western Blotting (WB) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr1248) antibody (ABIN4309121) Western Blot: ErbB2/Her2 [p Tyr1248] Antibody [ABIN43091210] - Analysis of extracts f...
Western Blotting (WB) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr1248) antibody (ABIN4309121) Western Blot: ErbB2/Her2 [p Tyr1248] Antibody [ABIN43091210] - Western blot analysis ...
Immunofluorescence (IF) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr1248) antibody (ABIN4309121) Immunocytochemistry/Immunofluorescence: ErbB2/Her2 [p Tyr1248] Antibody [ABIN43091210...
Western Blotting (WB) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr1248) antibody (ABIN4309121) Western Blot: ErbB2/Her2 [p Tyr1248] Antibody [ABIN43091210] - Analysis of extracts f...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr1248) antibody (ABIN4309121) Immunohistochemistry-Paraffin: ErbB2/Her2 [p Tyr1248] Antibody [ABIN43091210] - Human...