anti-Rat (Rattus) HER2 antibody for Immunoprecipitation

Recommended HER2 Antibody (supplied by: Log in to see )

Antigen
V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
  • CD340
  • HER-2
  • HER-2/neu
  • HER2
  • MLN 19
  • NEU
  • NGL
  • TKR1
  • Erbb-2
  • Neu
  • c-erbB2
  • c-neu
  • mKIAA3023
  • wu:fv70f10
  • zgc:63601
  • erb2
  • erb-b2 receptor tyrosine kinase 2
  • ERBB2
  • Erbb2
  • erbb2
Epitope
pTyr877
130
87
71
69
60
48
47
35
31
25
24
22
22
15
14
13
13
13
12
12
12
11
11
11
11
10
10
10
10
10
10
9
9
8
7
7
7
6
6
6
6
5
5
5
5
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1294
404
312
31
22
10
6
6
6
4
4
3
3
3
3
3
2
2
2
2
1
1
1
Host
Rabbit
772
584
23
16
10
3
3
Clonality
Polyclonal
Conjugate
This HER2 antibody is un-conjugated
57
44
44
42
41
27
18
18
11
11
11
10
10
9
8
8
8
8
8
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
6
2
2
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
671
452
412
410
351
192
192
102
100
98
95
47
24
21
18
11
6
3
3
3
2
1
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN6256054
$ 390.77
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13 ABIN1847484 IP IHC ELISA WB Rabbit pTyr877 Log in to see Polyclonal 0
7 ABIN2704879 IP IHC WB Rabbit C-Term Log in to see Polyclonal 0
7 ABIN1531861 ELISA IHC IP WB Rabbit IgG AA 851-900, pTyr877 Log in to see Polyclonal 0
7 ABIN1531859 ELISA IHC IP WB Rabbit IgG AA 1191-1240, pTyr1221 Log in to see Polyclonal 0
7 ABIN305740 IHC IHC (fro) IHC (p) IP WB Rabbit IgG AA 1243-1255 Log in to see Polyclonal 0
7 ABIN318038 EIA IHC (p) IP WB Rabbit pTyr877 Log in to see Polyclonal 0
7 ABIN318035 IHC (p) IP WB Rabbit pTyr1221, pTyr1222 Log in to see Polyclonal 0
7 ABIN1576967 IP IHC ELISA WB Rabbit pTyr877 Log in to see Polyclonal 0
7 ABIN187280 IHC (p) IP WB Mouse IgG1 Log in to see Cocktail 0
7 ABIN2990627 IHC IP WB Rabbit Log in to see Polyclonal 0
4 ABIN372478 IHC (fro) IHC (p) IP WB Rabbit IgG AA 1243-1255 Log in to see Polyclonal 0
4 ABIN5674092 IHC IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal 0
4 ABIN1846011 IP IHC WB Rabbit pTyr877 Log in to see Polyclonal 0
4 ABIN385644 IHC IHC (p) IP WB Mouse IgG1 Log in to see e2-4001 0
4 ABIN2470582 IP IHC WB Rabbit IgG AA 1243-1255 Log in to see Polyclonal 0
4 ABIN1734112 IHC (fro) IHC (p) IP WB Rabbit IgG AA 1243-1255 Log in to see Polyclonal 0
4 ABIN5506877 ELISA IP WB Rabbit IgG AA 4-656 Log in to see Polyclonal 0
1 ABIN2755561 IC IF IP IHC WB Rabbit C-Term, pTyr1248 Log in to see Polyclonal 0
1 ABIN2962674 FACS IHC (fro) IHC (p) IP WB Mouse IgG1 C-Term Log in to see 3B5 0
1 ABIN219136 IP IHC WB Mouse IgG1 Cytoplasmic Domain Log in to see 0

General

Antigen V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) Antibodies
Epitope pTyr877
(130), (87), (71), (69), (60), (48), (47), (35), (31), (25), (24), (22), (22), (15), (14), (13), (13), (13), (12), (12), (12), (11), (11), (11), (11), (10), (10), (10), (10), (10), (10), (9), (9), (8), (7), (7), (7), (6), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1294), (404), (312), (31), (22), (10), (6), (6), (6), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(772), (584), (23), (16), (10), (3), (3)
Clonality
Polyclonal
Conjugate This HER2 antibody is un-conjugated
(57), (44), (44), (42), (41), (27), (18), (18), (11), (11), (11), (10), (10), (9), (8), (8), (8), (8), (8), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (2), (2), (1)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(671), (452), (412), (410), (351), (192), (192), (102), (100), (98), (95), (47), (24), (21), (18), (11), (6), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-HER2 Antibody

Target Details HER2 Application Details Handling Images
Specificity Phospho-HER2 (Tyr877) Antibody detects endogenous levels of HER2 only when phosphorylated at Tyrosine 877
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen A synthesized peptide derived from human HER2 around the phosphorylation site of Tyrosine 877
Isotype IgG
Plasmids, Primers & others

Target Details HER2

Product Details anti-HER2 Antibody Application Details Handling Images back to top
Antigen
Alternative Name HER2 (ERBB2 Antibody Abstract)
Background

Description: Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization.

Gene: ERBB2

Molecular Weight 185kDa
Gene ID 2064
UniProt P04626
Pathways RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development

Application Details

Product Details anti-HER2 Antibody Target Details HER2 Handling Images back to top
Application Notes WB 1:500-1:2000 IHC 1:50-1:200 IP 1:100-1:500, IF/ICC 1:100-1:500
Restrictions For Research Use only

Handling

Product Details anti-HER2 Antibody Target Details HER2 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

Images

Product Details anti-HER2 Antibody Target Details HER2 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr877) antibody (ABIN6256054) Western blot analysis of Phospho-HER2 (Tyr877) expression in various lysates
Immunohistochemistry (IHC) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr877) antibody (ABIN6256054) ABIN6267284 at 1/100 staining mouse gastric tissue sections by IHC-P. The tissue was ...
Immunohistochemistry (IHC) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr877) antibody (ABIN6256054) ABIN6267284 at 1/100 staining mouse kidney tissue sections by IHC-P. The tissue was f...
Immunohistochemistry (IHC) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr877) antibody (ABIN6256054) ABIN6267284 at 1/100 staining mouse testicular tissue sections by IHC-P. The tissue w...
Immunohistochemistry (IHC) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr877) antibody (ABIN6256054) ABIN6267284 at 1/100 staining human pancreatic tissue sections by IHC-P. The tissue w...
Immunohistochemistry (IHC) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr877) antibody (ABIN6256054) ABIN6267284 at 1/100 staining human heart tissue sections by IHC-P. The tissue was fo...
Immunohistochemistry (IHC) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr877) antibody (ABIN6256054) ABIN6267284 at 1/100 staining human tongue cancer tissue sections by IHC-P. The tissu...
Immunohistochemistry (IHC) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr877) antibody (ABIN6256054) ABIN6267284 at 1/100 staining rat uterine cancer tissue sections by IHC-P. The tissue...
Immunohistochemistry (IHC) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr877) antibody (ABIN6256054) ABIN6267284 at 1/100 staining rat gastric cancer tissue sections by IHC-P. The tissue...
Immunohistochemistry (IHC) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr877) antibody (ABIN6256054) ABIN6267284 at 1/100 staining rat ovarian cancer tissue sections by IHC-P. The tissue...
Immunofluorescence (fixed cells) (IF/ICC) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr877) antibody (ABIN6256054) ABIN6267284 staining MDA-MB-231 by IF/ICC. The sample were fixed with PFA and permeab...
Western Blotting (WB) image for anti-V-Erb-B2 erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/glioblastoma Derived Oncogene Homolog (Avian) (ERBB2) (pTyr877) antibody (ABIN6256054) Western blot analysis of HER2 phosphorylation expression in EGF treated MDA-MB-231 wh...