anti-Human ROR1 antibody for Flow Cytometry

Recommended ROR1 Antibody (supplied by: Log in to see )

Antigen
Receptor Tyrosine Kinase-Like Orphan Receptor 1 (ROR1) Antibodies
  • NTRKR1
  • dJ537F10.1
  • 2810404D04Rik
  • Ntrkr1
  • mRor1
  • RGD1559469
  • ROR1
  • fi34e02
  • si:dkey-209n16.1
  • wu:fi34e02
  • receptor tyrosine kinase like orphan receptor 1
  • receptor tyrosine kinase-like orphan receptor 1
  • ROR1
  • Ror1
  • ror1
Epitope
AA 24-403
18
18
14
11
9
7
7
6
5
5
4
3
2
2
2
1
1
1
1
Reactivity
Human
126
55
46
5
3
3
3
3
2
2
1
1
1
1
1
Host
Goat
117
33
17
Clonality
Polyclonal
Conjugate
This ROR1 antibody is un-conjugated
10
10
7
7
7
5
4
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Simple Western (SimWes), Western Blotting (WB)
129
62
41
33
14
11
9
5
4
4
3
2
1
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Catalog No. ABIN4899817
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.363172 ABIN392042 FACS WB Rabbit Ig Fraction AA 32-62, N-Term Log in to see Polyclonal 2
13.363172 ABIN392043 FACS IHC (p) WB Rabbit Ig Fraction AA 112-399 Log in to see Polyclonal 0
13.363172 ABIN4896734 FACS APC Goat IgG AA 24-403 Log in to see Polyclonal 0
13.363172 ABIN4896737 FACS PE Goat IgG AA 24-403 Log in to see Polyclonal 0
13.363172 ABIN4896736 FACS Alexa Fluor 488 Goat IgG AA 24-403 Log in to see Polyclonal 0
13.363172 ABIN4896735 FACS PerCP Goat IgG AA 24-403 Log in to see Polyclonal 0
13.363172 ABIN2660230 FACS PerCP-Cy5.5 Mouse IgG1 kappa Log in to see 2A2 7
13.363172 ABIN2658385 FACS IF ICFC APC Mouse IgG1 kappa Log in to see 2A2 7
13.363172 ABIN2659390 FACS IP IHC ELISA PE-Cy7 Mouse IgG1 kappa Log in to see 2A2 7
13.363172 ABIN2662839 FACS PE Mouse IgG1 kappa Log in to see 2A2 7
13.363172 ABIN2665348 FACS Mouse IgG1 kappa Log in to see 2A2 7
13.363172 ABIN3032441 FACS ELISA WB Rabbit Ig Fraction AA 32-62 Log in to see Polyclonal 0
13.363172 ABIN3032439 FACS IHC ELISA WB Rabbit Ig Fraction Log in to see Polyclonal 0
1 ABIN2689893 IHC (fro) FACS IHC (p) IP Mouse IgG2b, kappa Log in to see 4A5 5
1 ABIN5534646 FACS WB Rabbit Ig Fraction AA 32-62, N-Term Log in to see Polyclonal 0
1 ABIN5534647 FACS IHC (p) WB Rabbit Ig Fraction Log in to see Polyclonal 0
1 ABIN1832360 FACS PerCP Goat IgG Log in to see Polyclonal 0
1 ABIN1931429 FACS ELISA WB APC Rabbit IgG AA 32-62 Log in to see Polyclonal 0
1 ABIN1931432 FACS ELISA WB HRP Rabbit IgG AA 32-62 Log in to see Polyclonal 0
1 ABIN1931430 FACS ELISA WB Biotin Rabbit IgG AA 32-62 Log in to see Polyclonal 0

General

Antigen Receptor Tyrosine Kinase-Like Orphan Receptor 1 (ROR1) Antibodies
Epitope AA 24-403
(18), (18), (14), (11), (9), (7), (7), (6), (5), (5), (4), (3), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human
(126), (55), (46), (5), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Goat
(117), (33), (17)
Clonality
Polyclonal
Conjugate This ROR1 antibody is un-conjugated
(10), (10), (7), (7), (7), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Simple Western (SimWes), Western Blotting (WB)
(129), (62), (41), (33), (14), (11), (9), (5), (4), (4), (3), (2), (1)
Pubmed 5 references available
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Product Details anti-ROR1 Antibody

Target Details ROR1 Application Details Handling References for anti-ROR1 antibody (ABIN4899817) Images
Specificity Detects human ROR1 in direct ELISAs and Western blots. In direct ELISAs, less than 5 % cross-reactivity with recombinant human ROR2 is observed.
Purification Antigen Affinity-purified
Sterility 0.2 μm filtered
Immunogen Mouse myeloma cell line NS0-derived recombinant human ROR1, Ala24-Glu403, Accession # Q01973
Isotype IgG
Plasmids, Primers & others

Target Details ROR1

Product Details anti-ROR1 Antibody Application Details Handling References for anti-ROR1 antibody (ABIN4899817) Images back to top
Antigen
Alternative Name ROR1 (ROR1 Antibody Abstract)
Background Alternative Names: EC 2.7.10.1, MGC99659, neurotrophic tyrosine kinase receptor-related 1, Neurotrophic tyrosine kinase, receptor-related 1, NTRKR1, NTRKR1dJ537F10.1, receptor tyrosine kinase-like orphan receptor 1, tyrosine-protein kinase transmembrane receptor ROR1
Gene ID 4919
UniProt Q01973
Pathways RTK Signaling, WNT Signaling, Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha

Application Details

Product Details anti-ROR1 Antibody Target Details ROR1 Handling References for anti-ROR1 antibody (ABIN4899817) Images back to top
Application Notes Western Blot 1 μg/mL
Simple Western 25 μg/mL
Flow Cytometry 0.25 μg/10^6 cells
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-ROR1 Antibody Target Details ROR1 Application Details References for anti-ROR1 antibody (ABIN4899817) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-ROR1 antibody (ABIN4899817)

Product Details anti-ROR1 Antibody Target Details ROR1 Application Details Handling Images back to top
Product cited in:

Yamaguchi, Lu, Ida, Yanagisawa, Usukura, Cheng, Hotta, Shimada, Isomura, Suzuki, Fujimoto, Takahashi: "ROR1 sustains caveolae and survival signalling as a scaffold of cavin-1 and caveolin-1." in: Nature communications, Vol. 7, pp. 10060, 2016 Method employed by authors: Immunoprecipitation (IP) (Sample species: Human).

Henry, Llamosas, Knipprath-Meszaros, Schoetzau, Obermann, Fuenfschilling, Caduff, Fink, Hacker, Ward, Heinzelmann-Schwarz, Ford: "Targeting the ROR1 and ROR2 receptors in epithelial ovarian cancer inhibits cell migration and invasion." in: Oncotarget, Vol. 6, Issue 37, pp. 40310-26, 2015 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).

Gentile, Lazzari, Benvenuti, Trusolino, Comoglio: "Ror1 is a pseudokinase that is crucial for Met-driven tumorigenesis." in: Cancer research, Vol. 71, Issue 8, pp. 3132-41, 2011 Method employed by authors: Immunoprecipitation (IP), Immunoprecipitation (IP) (Sample species: Human).

Hudecek, Schmitt, Baskar, Lupo-Stanghellini, Nishida, Yamamoto, Bleakley, Turtle, Chang, Greisman, Wood, Maloney, Jensen, Rader, Riddell: "The B-cell tumor-associated antigen ROR1 can be targeted with T cells modified to express a ROR1-specific chimeric antigen receptor." in: Blood, Vol. 116, Issue 22, pp. 4532-41, 2010 Method employed by authors: Flow Cytometry (FACS), Flow Cytometry (FACS) (Sample species: Human).

Baskar, Kwong, Hofer, Levy, Kennedy, Lee, Staudt, Wilson, Wiestner, Rader: "Unique cell surface expression of receptor tyrosine kinase ROR1 in human B-cell chronic lymphocytic leukemia." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 14, Issue 2, pp. 396-404, 2008 Method employed by authors: Flow Cytometry (FACS), Flow Cytometry (FACS) (Sample species: Human).

Images

Product Details anti-ROR1 Antibody Target Details ROR1 Application Details Handling References for anti-ROR1 antibody (ABIN4899817) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Receptor Tyrosine Kinase-Like Orphan Receptor 1 (ROR1) (AA 24-403) antibody (ABIN4899817) Detection of ROR1 in MDA-MB-231 Human Cell Line by Flow Cytometry. MDA-MB-231 human b...
Simple Western (SimWes) image for anti-Receptor Tyrosine Kinase-Like Orphan Receptor 1 (ROR1) (AA 24-403) antibody (ABIN4899817) Detection of Human ROR1 by Simple WesternTM. Simple Western lane view shows lysates o...
Western Blotting (WB) image for anti-Receptor Tyrosine Kinase-Like Orphan Receptor 1 (ROR1) (AA 24-403) antibody (ABIN4899817) Detection of ROR1 by Western Blot. Western blot shows lysate of NTera-2 human testicu...