|Antigen||Son of Sevenless Homolog 1 (Drosophila) (SOS1) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This SOS1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-SOS1 AntibodyTarget Details SOS1 Application Details Handling References for anti-SOS1 antibody (ABIN238504) Images
|Specificity||The antibody SOS-01 reacts with human Sos, an ubiquitously expressed 150 kDa intracellular protein.|
|Characteristics||Synonyms: SOS-1, Son of sevenless homolog 1|
|Purification||Protein A affinity chromatography.|
|Immunogen||Peptide corresponding to amino acids THPSMHRDGPPLLENAHSS of human Sos protein.|
Target Details SOS1Product Details anti-SOS1 Antibody Application Details Handling References for anti-SOS1 antibody (ABIN238504) Images back to top
|Alternative Name||SOS1 (SOS1 Antibody Abstract)|
|Background||The guanine nucleotide exchange factor Sos (Son-of-sevenless) is a complex multidomain protein that activates the small GTPase Ras (H-Ras, K-Ras, N-Ras, but not functionally distinct R-Ras) in response to receptor tyrosine kinase stimulation. Nucleotide exchange activity of Sos is stimulated by allosteric Ras binding. By another (separable) guanine exchange factor domain domain Sos modulates activity of Rac/Rho GTPases. Sos thus integrates signals that affect both gene expression and cytoskeletal reorganization, the Sos-mediated Ras-activation and Rac activation differ in composition and stability of the formed complex.Synonyms: SOS-1, Son of sevenless homolog 1|
|Pathways||RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor|
Application DetailsProduct Details anti-SOS1 Antibody Target Details SOS1 Handling References for anti-SOS1 antibody (ABIN238504) Images back to top
Western Blotting under reducing conditions (1 μg/mL): Positive control: HeLa human cervix carcinoma cell line. Immunocytochemistry. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-SOS1 Antibody Target Details SOS1 Application Details References for anti-SOS1 antibody (ABIN238504) Images back to top
|Buffer||PBS, pH 7.4 with 15 mM sodium azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid freeze/thaw cycles.
Shelf life: One year from despatch.
|Expiry Date||12 months|
References for anti-SOS1 antibody (ABIN238504)Product Details anti-SOS1 Antibody Target Details SOS1 Application Details Handling Images back to top
Freedman, Sondermann, Friedland, Kortemme, Bar-Sagi, Marqusee, Kuriyan: "A Ras-induced conformational switch in the Ras activator Son of sevenless." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 45, pp. 16692-7, 2006
Yang, Bashaw: "Son of sevenless directly links the Robo receptor to rac activation to control axon repulsion at the midline." in: Neuron, Vol. 52, Issue 4, pp. 595-607, 2006
Silver, Chen, Doyon, Zink, Rebay: "New class of Son-of-sevenless (Sos) alleles highlights the complexities of Sos function." in: Genesis (New York, N.Y. : 2000), Vol. 39, Issue 4, pp. 263-72, 2004
Innocenti, Tenca, Frittoli, Faretta, Tocchetti, Di Fiore, Scita: "Mechanisms through which Sos-1 coordinates the activation of Ras and Rac." in: The Journal of cell biology, Vol. 156, Issue 1, pp. 125-36, 2002
Tian, Feig: "Basis for signaling specificity difference between Sos and Ras-GRF guanine nucleotide exchange factors." in: The Journal of biological chemistry, Vol. 276, Issue 50, pp. 47248-56, 2001
ImagesProduct Details anti-SOS1 Antibody Target Details SOS1 Application Details Handling References for anti-SOS1 antibody (ABIN238504) back to top