anti-Horse (Equine) TRKB antibody for Immunohistochemistry

Recommended TRKB Antibody (supplied by: Log in to see )

Antigen
Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) Antibodies
  • trkb
  • trkb-b
  • xtrkb
  • si:ch211-220b11.1
  • trkB2
  • NTRK2
  • ntrk2
  • gp145-trkb
  • TRKB
  • GP145-TrkB
  • trk-B
  • AI848316
  • C030027L06Rik
  • Tkrb
  • trkB
  • RATTRKB1
  • TRKB1
  • Trk-B
  • TrkB
  • neurotrophic receptor tyrosine kinase 2
  • neurotrophic receptor tyrosine kinase 2 L homeolog
  • neurotrophic tyrosine kinase, receptor, type 2b
  • neurotrophic receptor tyrosine kinase 2 S homeolog
  • neurotrophic tyrosine kinase, receptor, type 2
  • NTRK2
  • ntrk2.L
  • ntrk2b
  • ntrk2.S
  • ntrk2
  • Ntrk2
Epitope
Cytoplasmic Domain
25
24
21
16
16
15
14
12
12
10
10
10
9
9
8
7
6
6
6
6
6
5
5
5
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Horse (Equine), Human, Monkey, Rabbit
339
203
189
13
12
9
8
8
3
2
1
1
1
1
Host
Rabbit
269
81
15
6
2
Clonality
Polyclonal
Conjugate
This TRKB antibody is un-conjugated
7
7
7
7
6
6
5
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
264
163
146
72
55
46
27
13
10
6
3
2
1
1
1
1
1
1
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Catalog No. ABIN1049153
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2784510 IF IHC WB Rabbit C-Term Log in to see Polyclonal 0

General

Antigen Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) Antibodies
Epitope Cytoplasmic Domain
(25), (24), (21), (16), (16), (15), (14), (12), (12), (10), (10), (10), (9), (9), (8), (7), (6), (6), (6), (6), (6), (5), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Horse (Equine), Human, Monkey, Rabbit
(339), (203), (189), (13), (12), (9), (8), (8), (3), (2), (1), (1), (1), (1)
Host Rabbit
(269), (81), (15), (6), (2)
Clonality
Polyclonal
Conjugate This TRKB antibody is un-conjugated
(7), (7), (7), (7), (6), (6), (5), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(264), (163), (146), (72), (55), (46), (27), (13), (10), (6), (3), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-TRKB Antibody

Target Details TRKB Application Details Handling Images
Brand IHC-plus™
Specificity Human NTRK2 / TRKB. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Panda, Horse, Rabbit (100%) Bovine (92%) Galago, Bat (83%).
Purification Immunoaffinity purified
Immunogen Synthetic 12 amino acid peptide from cytoplasmic domain of human NTRK2 / TRKB. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Panda, Horse, Rabbit (100%), Bovine (92%), Galago, Bat (83%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Target Details TRKB

Product Details anti-TRKB Antibody Application Details Handling Images back to top
Antigen
Alternative Name NTRK2 / TRKB (NTRK2 Antibody Abstract)
Background Name/Gene ID: NTRK2
Subfamily: Trk/Ror
Family: Protein Kinase

Synonyms: NTRK2, gp145-TrkB, Trk-B, TRKB, Tropomyosin-related kinase B, TrkB tyrosine kinase, Tyrosine kinase receptor B
Gene ID 4915
Pathways RTK Signaling, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Skeletal Muscle Fiber Development, Feeding Behaviour, Dicarboxylic Acid Transport

Application Details

Product Details anti-TRKB Antibody Target Details TRKB Handling Images back to top
Application Notes Approved: IHC, IHC-P (20 μg/mL)
Comment

Target Species of Antibody: Human

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

Product Details anti-TRKB Antibody Target Details TRKB Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months

Images

Product Details anti-TRKB Antibody Target Details TRKB Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Neurotrophic Tyrosine Kinase, Receptor, Type 2 (NTRK2) (Cytoplasmic Domain) antibody (ABIN1049153) Anti-NTRK2 / TRKB antibody ABIN1049153 IHC staining of human brain, neurons. Immunoh...