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Rabbit anti-Mouse IgG3 (Heavy Chain) Antibody - Preadsorbed

ELISA, WB, IHC Polyclonal IgG unconjugated
Catalog No. ABIN102433
  • Target See all IgG3 products
    IgG3
    Binding Specificity
    • 37
    • 15
    • 6
    • 5
    • 4
    • 1
    • 1
    • 1
    Heavy Chain
    Reactivity
    • 70
    • 19
    • 3
    Mouse
    Host
    • 37
    • 26
    • 18
    • 10
    • 1
    Rabbit
    Clonality
    • 63
    • 27
    Polyclonal
    Application
    • 50
    • 40
    • 36
    • 32
    • 25
    • 21
    • 11
    • 8
    • 6
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
    Specificity
    IgG3
    Characteristics
    Concentration Definition: by UV absorbance at 280 nm
    Purification
    Preadsorption: Solid phase absorption
    Sterility
    Sterile filtered
    Immunogen

    Immunogen: Mouse IgG3 heavy chain

    Isotype
    IgG
  • Application Notes

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Anti-Mouse IgG3 antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.

    ELISA Dilution: 1:10,000 - 1:50,000

    Western Blot Dilution: 1:1,000 - 1:5,000

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Expiry Date
    12 months
  • Target
    IgG3
    Abstract
    IgG3 Products
    Target Type
    Antibody
    Background

    Synonyms: Rabbit Anti-Mouse IgG3 (Gamma 3 chain) Antibody, Rabbit Anti Mouse IgG3 Antibody

    Background: Anti-Mouse IgG3 Antibody generated in rabbit detects reactivity to Mouse IgG3 (Gamma 3 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG3 comprises almost 10 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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