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Goat anti-Mouse IgG1 Antibody (Alkaline Phosphatase (AP))

WB, IHC (fro), EIA, IHC (p) Polyclonal IgG Alkaline Phosphatase (AP)

Catalog No. ABIN318483

Datasheet as PDF

Target See all IgG1 products

IgG1
Reactivity
84

24

22

7

7

5

4

4

3

2

1
Mouse
Host
62

38

34

10

9

6

1
Goat
Clonality
94

53
Polyclonal
Conjugate
43

18

18

16

9

8

7

4

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Alkaline Phosphatase (AP)
Application
74

54

50

39

32

21

16

13

11

10

10

9

5

4

3

2

2

1
Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

No Cross-Reactivity

Human

Cross-Reactivity (Details)

Species reactivity (tested):Mouse.

Purification

Affinity Chromatography

Immunogen

Mouse IgG1 paraproteins.

Isotype

IgG
Goat anti-Mouse IgG1 (Heavy Chain) antibody (HRP)
ELISA, WB, IHC Polyclonal IgG HRP

Rabbit anti-Mouse IgG1 (Heavy Chain) antibody - Preadsorbed
ELISA, WB, IHC Polyclonal IgG unconjugated

Goat anti-Mouse IgG1 (Heavy Chain) antibody (Alkaline Phosphatase (AP))
ELISA, WB, IHC Polyclonal IgG Alkaline Phosphatase (AP)

Goat anti-Mouse IgG1 (Heavy Chain) antibody
ELISA, WB, IHC, FACS Polyclonal IgG unconjugated

Goat anti-Mouse IgG1 (Heavy Chain) antibody (Biotin)
ELISA, WB, IHC, FACS Polyclonal IgG Biotin

Rabbit anti-Mouse IgG1 (Heavy Chain) antibody (Alkaline Phosphatase (AP))
ELISA, WB, IHC Polyclonal IgG Alkaline Phosphatase (AP)

Goat anti-Mouse IgG1 (Heavy Chain) antibody (Atto 647N) - Preadsorbed
WB, FACS, FLISA, FM Polyclonal IgG Atto 647N

Goat anti-Mouse IgG1 (Heavy Chain) antibody (FITC) - Preadsorbed
WB, IHC, FACS, FLISA, FM Polyclonal IgG FITC

Goat anti-Mouse IgG1 (Heavy Chain) antibody (Atto 488) - Preadsorbed
WB, FLISA, FM Polyclonal IgG Atto 488

Goat anti-Mouse IgG1 (Heavy Chain) antibody - Preadsorbed
ELISA, WB, IHC Polyclonal IgG unconjugated

Application Notes

ELISA: 1/2000-1/4000. Western Blot. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Format

Liquid

Concentration

1.0 mg/mL

Buffer

50 mM TRIS, 1 mM MgCl2, pH 8.0, 0.09 % Sodium Azide, 50 % Glycerol

Preservative

Sodium azide

Precaution of Use

WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice

Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage

4 °C

Storage Comment

Store the antibody undiluted at 2-8 °C.
Target

IgG1

Abstract

IgG1 Products

Target Type

Antibody

Background

Synonyms: Mouse Immunoglobulin G1