Goat anti-Mouse IgG1 (Heavy Chain) Antibody - Preadsorbed

Catalog No. ABIN1043977

Product Details

Target: IgG1

Binding Specificity: Heavy Chain

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum, Mouse IgG and Mouse IgG1. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA for human and rabbit. For IgG2a, IgG2b and IgG3 reactivity was less than 5% against target (IgG1, IgG). Cross-reactivity against Bovine is assessed only by IEP.

Cross-Reactivity: Mouse (Murine)

Characteristics: Mouse IgG1 secondary antibody is available in a variety of formats.

Purification: Preadsorption: Solid phase absorption

Sterility: Sterile filtered

Immunogen:

Immunogen: Mouse IgG1 heavy chain

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:2,000 - 1:10,000

Application Note: Anti-Mouse IgG1 secondary antibody is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG1 antibody based assays.

ELISA Dilution: 1:40,000 - 1:200,000

Western Blot Dilution: 1:4,000 - 1:20,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid cycles of freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature.

Storage: 4 °C,-20 °C

Storage Comment: Store second antibody at 4 °C prior to restoration. For extended storage aliquot secondary antibody and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.

Expiry Date: 12 months

Target Details

Target: IgG1

Abstract: IgG1 Products

Target Type: Antibody

Background:

Synonyms: Goat anti-mouse IgG1 antibody, goat anti-mouse IgG1 (gamma 1) antibody

Background: Anti-Mouse IgG1 Antibody generated in goat detects reactivity to Mouse IgG1 (Gamma 1 chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG1 chain constitutes 66 % of the IgG subclass and has a high affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.