Goat anti-Mouse IgA, IgG, IgM (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) - Preadsorbed

Catalog No. ABIN5608095

Product Details

Target: IgA, IgG, IgM

Fragment: F(ab')2 fragment

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Characteristics: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Mouse IgG IgA and IgM whole molecule

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:100 - 1:500

Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. This product has been assayed against 1.0 μg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

ELISA Dilution: 1:5,000

Western Blot Dilution: 1:200 - 1:1,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.

Expiry Date: 12 months

Target Details

Target: IgA, IgG, IgM

Alternative Name: IgG IgA IgM (IgA, IgG, IgM Products)

Target Type: Antibody

Background:

Synonyms: Goat F(ab')2 Anti-Mouse IgG IgA IgM Antibody Alkaline Phosphatase Conjugation, Goat Fab2 Anti-Mouse IgGAM alk phos Conjugated Antibody

Background: F(ab')2 Anti-Mouse IgG IgA IgM (H&L) Alkaline Phosphatase Antibody generated in goat detects reactivity to Mouse IgG, Mouse IgA, and Mouse IgM subclasses and chains. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.