Goat anti-Rat IgA, IgG, IgM (Heavy & Light Chain) Antibody (HRP) - Preadsorbed
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- Target See all IgA, IgG, IgM products
- IgA, IgG, IgM
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Binding Specificity
- Heavy & Light Chain
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Reactivity
- Rat
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- HRP
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Characteristics
- Rat IgG IgA IgM (H&L) Antibody detects immunoglobulin G, A, and M from Rat. Immunoglobulin G binds to antigens and can neutralize or opsinize targts, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunogobulin molecules linked through their F(c) domain by the J chain.
- Purification
- Preadsorption: Solid phase absorption
- Immunogen
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Immunogen: Rat IgG, IgA and IgM whole molecules
- Isotype
- IgG
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- Application Notes
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Immunohistochemistry Dilution: 1:500-1:1,500
Application Note: Anti-Rat IgG IgA IgM Antibody is suitable for immunoassays where reactivity to different classes of rat immunoglobulins is desired. Antibody has been tested by ELISA, western blot immunoblot, and immunohistochemistry. Optimal concentrations in immunoassays should be determined by the researcher.
ELISA Dilution: 1:100,000
Western Blot Dilution: 1:500-1:3,000
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 2.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Thimerosal
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- RT,4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- IgA, IgG, IgM
- Alternative Name
- IgG IgA IgM (IgA, IgG, IgM Products)
- Target Type
- Antibody
- Background
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Synonyms: goat anti-Rat IgG IgA IgM Antibody peroxidase conjugation, Goat Anti-rat IgGAM HRP conjugated antibody
Background: Rat IgG IgA IgM (H&L) Antibody detects immunoglobulin G, A, and M from Rat. Immunoglobulin G binds to antigens and can neutralize or opsinize targts, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunogobulin molecules linked through their F(c) domain by the J chain.
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