Goat anti-Rat IgA, IgG, IgM (Heavy & Light Chain) Antibody (Biotin) - Preadsorbed
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- Target See all IgA, IgG, IgM products
- IgA, IgG, IgM
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Binding Specificity
- Heavy & Light Chain
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Reactivity
- Rat
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- Biotin
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Cross-Reactivity
- Rat (Rattus)
- Characteristics
- Anti-Rat IgG IgA IgM (H&L) biotin conjugated antibody generated in goat detects specifically rat IgG IgA IgM (H&L). This secondary biotin conjugated antibody anti-Rat is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
- Purification
- Preadsorption: Solid phase absorption
- Immunogen
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Immunogen: Anti-Rat IgG IgA IgM (H&L) was produced by repeated immunization with Rat IgG, IgA and IgM whole molecules in goat.
Immunogen Type: Native Protein
- Isotype
- IgG
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- Application Notes
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Immunohistochemistry Dilution: 1:1,000 - 1:10,000
Application Note: Anti-Rat IgG IgA IgM (H&L) Biotin conjugated antibodies are suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
ELISA Dilution: 1:150,000
Western Blot Dilution: 1:2,000 - 1:20,000
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 2.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- RT,4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
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- Target
- IgA, IgG, IgM
- Alternative Name
- IgG IgA IgM (IgA, IgG, IgM Products)
- Target Type
- Antibody
- Background
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Synonyms: goat anti-Rat IgG IgA IgM Antibody biotin conjugation, Goat Anti-rat IgGAM biotin conjugated antibody
Background: Anti-Rat IgG IgA IgM (H&L) biotin conjugated antibody generated in goat detects specifically rat IgG IgA IgM (H&L). This secondary biotin conjugated antibody anti-Rat is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
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