anti-Pig (Porcine) Aurora Kinase B antibody for Enzyme Immunoassay

Recommended Aurora Kinase B Antibody

Antigen
Aurora Kinase B (AURKB) Antibodies
  • AIK2
  • AIM-1
  • AIM1
  • ARK2
  • AurB
  • IPL1
  • PPP1R48
  • STK12
  • STK5
  • aurkb-sv1
  • aurkb-sv2
  • AIRK2
  • AL022959
  • Aik2
  • Aim1
  • Ark2
  • STK-1
  • Stk12
  • Stk5
  • Aur B
  • Aurora B
  • Aurora-B
  • CG6620
  • DmAIRK2
  • DmAurB
  • DmAuroraB
  • Dmel\\CG6620
  • IAL
  • IAL1
  • Ial
  • aurB
  • aurora B
  • ARK-2
  • XAIRK2
  • aik2
  • aim-1
  • aim1
  • airk2
  • ark2
  • ipl1
  • stk12
  • stk5
  • CHUNP6896
  • fa09g06
  • stka
  • wu:fa09g06
  • aurkb
  • AIRK2-B
  • XAIRK2-B
  • aurkb-a
  • aurkb-b
  • xAurora-B-B
  • aurora kinase B
  • aurora B
  • aurora kinase B S homeolog
  • AURKB
  • Aurkb
  • aurB
  • aurkb
  • aurkb.S
Epitope
AA 223-234, pThr232
32
32
27
21
16
13
12
10
10
9
7
7
7
7
6
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Chimpanzee, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
279
119
91
17
7
6
3
2
2
1
1
1
1
Host
Rabbit
215
60
7
7
Clonality
Polyclonal
Conjugate
This Aurora Kinase B antibody is un-conjugated
12
9
9
8
8
7
3
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
229
143
97
93
43
32
15
12
11
9
8
2
2
1
Options
Catalog No. ABIN5551742
$ 632.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
1 ABIN318827 EIA WB Rabbit IgG AA 227-238 Polyclonal 2

General

Antigen Aurora Kinase B (AURKB) Antibodies
Epitope AA 223-234, pThr232
(32), (32), (27), (21), (16), (13), (12), (10), (10), (9), (7), (7), (7), (7), (6), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chimpanzee, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
(279), (119), (91), (17), (7), (6), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(215), (60), (7), (7)
Clonality
Polyclonal
Conjugate This Aurora Kinase B antibody is un-conjugated
(12), (9), (9), (8), (8), (7), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(229), (143), (97), (93), (43), (32), (15), (12), (11), (9), (8), (2), (2), (1)
Supplier

Product Details anti-Aurora Kinase B Antibody

Target Details Aurora Kinase B Application Details Handling Images
Specificity The product was affinity purified from monospecific antiserum by Immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. This antibody is directed against the phosphorylated form of human Aurora Kinase B at the pT232 residue. Reactivity with non-phosphorylated human Aurora Kinase B is minimal by ELISA. No reaction is expected against Aurora Kinase A. Cross reactivity with Aurora Kinase B from other sources is not known.
Cross-Reactivity (Details) Species reactivity (expected):Mouse, Rat, Bovine, Pig, Dog and Chimpanzee (100 %).
Species reactivity (tested):Human.
Characteristics Concentration: 1.21 mg/mL (by UV absorbance at 280 nm)
Purification Affinity Chromatography.
Immunogen Synthetic peptide corresponding aa 223-234 of Human Aurora Kinase B protein
Isotype IgG
Plasmids, Primers & others

Target Details Aurora Kinase B

Product Details anti-Aurora Kinase B Antibody Application Details Handling Images back to top
Antigen
Alternative Name Aurora Kinase B (AURKB Antibody Abstract)
Background Aurora Kinase B (also known as Aurora and Ipl1-like midbody-associated protein 1, AIM-1, Aurora/IPL1-related kinase 2, Aurora-related kinase 2, STK-1, and Aurora-B) is a Ser/Thr protein kinase member of the Aurora subfamily that may be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Aurora Kinase B is localized to the midzone of central spindle in late anaphase and concentrated into the midbody in telophase and cytokinesis and is colocalized with gamma tubulin in the mid-body. High levels of Aurora B expression are seen in the thymus, although it is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. Aurora B is expressed during S and G2/M phase and expression is up-regulated in cancer cells during M phase.Synonyms: AIK2, AIM1, ARK2, AURKB, Aurora- and Ipl1-like midbody-associated protein 1, Aurora-B, Aurora/IPL1-related kinase 2, STK-1, STK12, Serine/threonine-protein kinase 12
Gene ID 9212
NCBI Accession NP_004208
UniProt Q96GD4
Pathways TCR Signaling, Cell Division Cycle, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades

Application Details

Product Details anti-Aurora Kinase B Antibody Target Details Aurora Kinase B Handling Images back to top
Application Notes ELISA: 1/10,000-1/50,000. Western blot: 1/250-1/2,000 (See protocol below). Expect a band approx. 39 kDa in size corresponding to Aurora Kinase B by western blotting in theappropriate cell lysate or extract. HeLa cell lysate can be used as a positive control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Aurora Kinase B Antibody Target Details Aurora Kinase B Application Details Images back to top
Concentration 1.21 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.