anti-Ferret CD8 antibody for ELISA (Detection)

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • cd8
  • LOC100136450
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • CD8
  • Leu2
  • MAL
  • p32
  • RHACD8-4
  • CD8a molecule
  • uncharacterized LOC100125537
  • CD8 alpha
  • CD8 antigen, alpha chain
  • T-cell surface glycoprotein CD8 alpha chain
  • Cd8a
  • LOC100125537
  • LOC100136450
  • CD8A
  • cd8a
  • LOC100126556
Epitope
AA 1-186
85
26
20
18
15
12
12
12
12
11
11
9
9
8
7
7
6
5
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Ferret
607
459
130
91
49
24
15
15
15
12
12
10
6
4
2
2
2
2
2
2
2
1
1
Host
Mouse
709
412
263
1
Clonality
Monoclonal
Conjugate
This CD8 antibody is un-conjugated
146
140
87
83
35
29
28
27
21
20
18
18
18
17
17
17
17
17
15
14
14
13
13
13
12
10
8
7
6
6
5
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, ELISA (Detection)
1129
441
302
257
255
217
215
158
147
29
26
23
20
13
13
11
10
6
6
5
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN2001457
$ 218.90
Plus shipping costs $45.00

General

Antigen CD8a Molecule (CD8A) Antibodies
Epitope AA 1-186
(85), (26), (20), (18), (15), (12), (12), (12), (12), (11), (11), (9), (9), (8), (7), (7), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Ferret
(607), (459), (130), (91), (49), (24), (15), (15), (15), (12), (12), (10), (6), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Host Mouse
(709), (412), (263), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is un-conjugated
(146), (140), (87), (83), (35), (29), (28), (27), (21), (20), (18), (18), (18), (17), (17), (17), (17), (17), (15), (14), (14), (13), (13), (13), (12), (10), (8), (7), (6), (6), (5), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, ELISA (Detection)
(1129), (441), (302), (257), (255), (217), (215), (158), (147), (29), (26), (23), (20), (13), (13), (11), (10), (6), (6), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling Images
Specificity Ferret CD8a
No cross-reactivity in ELISA with
Human CD8A
Mouse CD8A
Human cell lysate (293 cell line)
No Cross-Reactivity Human, Mouse (Murine)
Characteristics This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Ferret CD8a (rF CD8a, ABIN2008785 ABS50091.1, Met 1-Glu 186,. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Purification purified by Protein A affinity chromatography
Sterility 0.2 μm filtered
Immunogen Recombinant Ferret CD8a protein (ABIN2008785)
Clone 3B5H2
Isotype IgG1
Plasmids, Primers & others

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CD8A (CD8A Antibody Abstract)
Background Human T-cell surface glycoprotein CD8 alpha chain, also known as CD8a, is a single-pass type I, membrane protein. The CD8 glycoprotein is expressed by thymocytes, mature T cells and natural killer (NK) cells and has been implicated in the recognition of monomorphic determinants on major histocompatibility complex (MHC) Class I antigens, and in signal transduction during the course of T-cell activation. Both human and rodent CD8 antigens are comprised of two distinct polypeptide chains, alpha and beta. The Ig domains of CD8 alpha are involved in controlling the ability of CD8 to be expressed. Mutation of B- and F-strand cysteine residues in CD8 alpha reduced the ability of the protein to fold properly and, therefore, to be expressed. Defects in CD8A are a cause of familial CD8 deficiency. Familial CD8 deficiency is a novel autosomal recessive immunologic defect characterized by absence of CD8+ cells, leading to recurrent bacterial infections.
UniProt ABS50091.1
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling Images back to top
Application Notes ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Ferret CD8A. The detection limit for Ferret CD8A is approximately 0.078 ng/well.
ELISA(Det): 0.1-1 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details Images back to top
Format Liquid
Buffer 0.2 μm filtered solution in PBS with 5 % trehalose
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C,-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Expiry Date 12 months