anti-Mouse (Murine) CD8 antibody for Immunocytochemistry

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • cd8
  • LOC100136450
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • CD8
  • Leu2
  • MAL
  • p32
  • RHACD8-4
  • CD8a molecule
  • uncharacterized LOC100125537
  • CD8 alpha
  • CD8 antigen, alpha chain
  • T-cell surface glycoprotein CD8 alpha chain
  • Cd8a
  • LOC100125537
  • LOC100136450
  • CD8A
  • cd8a
  • LOC100126556
Reactivity
Mouse (Murine)
607
457
130
91
49
24
15
15
15
13
12
10
6
4
2
2
2
2
2
2
2
1
1
Host
Rat
710
410
263
1
Clonality
Monoclonal
Conjugate
This CD8 antibody is conjugated to PE
146
139
87
83
35
29
28
27
21
20
18
18
17
17
17
17
17
17
15
14
14
13
13
13
12
10
8
7
6
6
5
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Functional Studies (Func), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
1127
441
302
256
255
216
215
157
147
28
26
23
20
13
13
10
10
6
6
5
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
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Catalog No. ABIN349711
$ 331.68
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
24.755764 ABIN457303 FACS Func ICC IHC (f) IHC (p) IP APC Rat IgG2a Log in to see 53-6-7 7
24.561531 ABIN4900660 FACS ICC Biotin Rat IgG2a Log in to see 53-6-7 2
21.755764 ABIN349708 FACS Func ICC IHC (f) IHC (p) IP FITC Rat IgG2a Log in to see 53-6-7 9
21.755764 ABIN2749035 FACS Func ICC IHC (f) IHC (p) IP Rat IgG2a Log in to see 53-6-7 8
21.755764 ABIN2749187 Func IHC (f) ICC FACS IP IHC (p) Rat IgG2a Log in to see 53-6-7 8
21.561531 ABIN4900730 CyTOF FACS ICC IP Rat IgG2a Log in to see 53-6-7 2
17.5 ABIN5693154 ELISA FACS ICC IHC WB Rabbit AA 28-196 Log in to see Polyclonal 2
7 ABIN1862305 ICC IHC WB Rabbit IgG AA 27-247 Log in to see Polyclonal 0
7 ABIN4898431 FACS ICC Alexa Fluor 488 Rat IgG2a Log in to see 53-6-7 0
1 ABIN3200064 FACS Func ICC IHC IP PerCP Rat IgG2a Log in to see 53-6-7 0
1 ABIN3200061 FACS Func ICC IHC IP APC Rat IgG2a Log in to see 53-6-7 0
1 ABIN3200063 FACS Func ICC IHC IP PE Rat IgG2a Log in to see 53-6-7 0
1 ABIN3200062 FACS Func ICC IHC IP FITC Rat IgG2a Log in to see 53-6-7 0
1 ABIN3200059 FACS Func ICC IHC IP Rat IgG2a Log in to see 53-6-7 0
1 ABIN3200060 FACS Func ICC IHC IP Rat IgG2a Log in to see 53-6-7 0

General

Antigen CD8a Molecule (CD8A) Antibodies
Reactivity Mouse (Murine)
(607), (457), (130), (91), (49), (24), (15), (15), (15), (13), (12), (10), (6), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Host Rat
(710), (410), (263), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD8 antibody is conjugated to PE
(146), (139), (87), (83), (35), (29), (28), (27), (21), (20), (18), (18), (17), (17), (17), (17), (17), (17), (15), (14), (14), (13), (13), (13), (12), (10), (8), (7), (6), (6), (5), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Functional Studies (Func), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
(1127), (441), (302), (256), (255), (216), (215), (157), (147), (28), (26), (23), (20), (13), (13), (10), (10), (6), (6), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 8 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 antibody (ABIN349711) Images
Specificity The rat monoclonal antibody 53-6.7 recognizes mouse CD8a (32-34 kDa, alpha chain of the CD8 antigen).
Characteristics The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Immunogen Mouse spleen cells
Clone 53-6-7
Isotype IgG2a
Plasmids, Primers & others

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 antibody (ABIN349711) Images back to top
Antigen
Alternative Name CD8A (CD8A Antibody Abstract)
Background The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 antibody (ABIN349711) Images back to top
Application Notes The reagent is designed for Flow Cytometry analysis.Suggested working concentration is 2 µ,g/mL. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 antibody (ABIN349711) Images back to top
Concentration 0.5 mg/mL
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

References for anti-CD8 antibody (ABIN349711)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Product cited in:

Mochimaru, Usui, Yaguchi, Nagahama, Hasegawa, Usui, Shimmura, Tsubota, Amano, Kawakami, Ishida: "Suppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2." in: Investigative ophthalmology & visual science, Vol. 49, Issue 5, pp. 2172-7, 2008

Bouwer, Alberti-Segui, Montfort, Berkowitz, Higgins: "Directed antigen delivery as a vaccine strategy for an intracellular bacterial pathogen." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 13, pp. 5102-7, 2006

Kamimura, Sawa, Sato, Agung, Hirano, Murakami: "IL-2 in vivo activities and antitumor efficacy enhanced by an anti-IL-2 mAb." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 177, Issue 1, pp. 306-14, 2006

Hata, Sakaguchi, Yoshitomi, Iwakura, Sekikawa, Azuma, Kanai, Moriizumi, Nomura, Nakamura, Sakaguchi: "Distinct contribution of IL-6, TNF-alpha, IL-1, and IL-10 to T cell-mediated spontaneous autoimmune arthritis in mice." in: The Journal of clinical investigation, Vol. 114, Issue 4, pp. 582-8, 2004

Grabbe, Varga, Beissert, Steinert, Pendl, Seeliger, Bloch, Peters, Schwarz, Sunderkötter, Scharffetter-Kochanek: "Beta2 integrins are required for skin homing of primed T cells but not for priming naive T cells." in: The Journal of clinical investigation, Vol. 109, Issue 2, pp. 183-92, 2002

Takahashi, Nakata, Tanaka, Adachi, Nakauchi, Yagita, Okumura: "CD4 and CD8 regulate interleukin 2 responses of T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 12, pp. 5557-61, 1992

Ledbetter, Herzenberg: "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." in: Immunological reviews, Vol. 47, pp. 63-90, 1980

Ledbetter, Rouse, Micklem, Herzenberg: "T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views." in: The Journal of experimental medicine, Vol. 152, Issue 2, pp. 280-95, 1980

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 antibody (ABIN349711) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD8a Molecule (CD8A) antibody (PE) (ABIN349711) Surface staining of murine splenocytes with anti-CD8a (53-6.7) PE.