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|Antigen||CD8a Molecule (CD8A) Antibodies|
|Reactivity||Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CD8 antibody is un-conjugated Alternatives|
Cytometry by Time of Flight (CyTOF), Depletion (Dep), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Inhibition Assay (InhA)
|9 references available|
|Supplier||Log in to see|
Product Details anti-CD8 AntibodyTarget Details CD8 Application Details Handling References for anti-CD8 antibody (ABIN4295972) Images
|Purification||Protein A or G purified|
|Immunogen||Mouse thymus or spleen.|
|Plasmids, Primers & others|
Target Details CD8Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 antibody (ABIN4295972) Images back to top
|Alternative Name||CD8 alpha (CD8A Antibody Abstract)|
|Background||Gene Symbol: CD8A|
|Molecular Weight||Theoretical MW: 27 kDa|
Application DetailsProduct Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 antibody (ABIN4295972) Images back to top
|Application Notes||Flow Cytometry 1:10 - 1:1000, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunoprecipitation 1:10 - 1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, Immunohistochemistry-Frozen 1:10 - 1:500, Cell depletion, Inhibition of T Cell Function, CyTOF-readyEach lot of this CD8a antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is <0.25 μg/10^6 cells in 100 μL volume. It is recommended that the reagent be titrated for optimal performance for each application. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a+ cells. Additional reported applications (for the relevant formats) include: immunoprecipitation, in vivo and in vitro cell depletion, inhibition of CD8 T cell proliferation, blocking of cytotoxicity, and immunohistochemical staining of both acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections. Immunocytochemistry/Immunofluorescence was reported in scientific literature. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24565643)|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 antibody (ABIN4295972) Images back to top
Buffer contains: 0.02 % Sodium Azide
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C,-20 °C|
|Storage Comment||Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.|
References for anti-CD8 antibody (ABIN4295972)Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
|Product cited in:||
Cohen, Shani, Raz, Sharon, Hoffman, Abramovitz, Erez: "Fibroblasts drive an immunosuppressive and growth-promoting microenvironment in breast cancer via secretion of Chitinase 3-like 1." in: Oncogene, Vol. 36, Issue 31, pp. 4457-4468, 2017 Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Mouse (Murine)).
Sierra, Trillo-Tinoco, Mohamed, Yu, Achyut, Arbab, Bradford, Osborne, Miele, Rodriguez: "Anti-Jagged Immunotherapy Inhibits MDSCs and Overcomes Tumor-Induced Tolerance." in: Cancer research, Vol. 77, Issue 20, pp. 5628-5638, 2017 Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Mouse (Murine)).
Li, Wu, Tang, Li, Feng, Yao: "In Vivo Magnetic Resonance Imaging of CD8+ T Lymphocytes Recruiting to Glioblastoma in Mice." in: Cancer biotherapy & radiopharmaceuticals, Vol. 31, Issue 9, pp. 317-323, 2016 (Sample species: Human).
Fazio, Zappulla, Notartomaso, Busceti, Bessede, Scarselli, Vacca, Gargaro, Volpi, Allegrucci, Lionetto, Simmaco, Belladonna, Nicoletti, Fallarino: "Cinnabarinic acid, an endogenous agonist of type-4 metabotropic glutamate receptor, suppresses experimental autoimmune encephalomyelitis in mice." in: Neuropharmacology, Vol. 81, pp. 237-43, 2014 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)
Kheradmand, Wang, Gibly, Zhang, Holland, Tasch, Graham, Kaufman, Miller, Shea, Luo: "Permanent protection of PLG scaffold transplanted allogeneic islet grafts in diabetic mice treated with ECDI-fixed donor splenocyte infusions." in: Biomaterials, Vol. 32, Issue 20, pp. 4517-24, 2011 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry
Bankoti, Burnett, Navarro, Miller, Rase, Shepherd: "Effects of TCDD on the fate of naive dendritic cells." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 115, Issue 2, pp. 422-34, 2010
McLellan, Kapp, Eggert, Linden, Bommhardt, Bröcker, Kämmerer, Kämpgen: "Anatomic location and T-cell stimulatory functions of mouse dendritic cell subsets defined by CD4 and CD8 expression." in: Blood, Vol. 99, Issue 6, pp. 2084-93, 2002
Takahashi, Nakata, Tanaka, Adachi, Nakauchi, Yagita, Okumura: "CD4 and CD8 regulate interleukin 2 responses of T cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 12, pp. 5557-61, 1992
Ledbetter, Herzenberg: "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." in: Immunological reviews, Vol. 47, pp. 63-90, 1980
ImagesProduct Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 antibody (ABIN4295972) back to top