anti-Mouse (Murine) CD8 antibody for Immunohistochemistry

Recommended CD8 Antibody (supplied by: Log in to see )

Antigen
CD8a Molecule (CD8A) Antibodies
  • cd8
  • LOC100136450
  • BB154331
  • Ly-2
  • Ly-35
  • Ly-B
  • Lyt-2
  • zgc:136643
  • CD8
  • Leu2
  • MAL
  • p32
  • RHACD8-4
  • CD8a molecule
  • uncharacterized LOC100125537
  • CD8 alpha
  • CD8 antigen, alpha chain
  • T-cell surface glycoprotein CD8 alpha chain
  • Cd8a
  • LOC100125537
  • LOC100136450
  • CD8A
  • cd8a
  • LOC100126556
Epitope
AA 28-196
81
26
19
17
15
13
12
12
12
11
11
9
9
8
7
7
6
5
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine), Rat (Rattus)
599
447
122
91
47
24
15
15
15
13
12
9
6
4
2
2
2
2
2
2
2
1
1
Host
Rabbit
695
399
261
1
Clonality
Polyclonal
Conjugate
This CD8 antibody is un-conjugated
144
138
85
81
33
28
27
26
21
19
18
18
17
17
17
17
17
17
15
14
14
12
12
11
11
10
8
7
6
5
5
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)
1101
429
294
251
249
209
207
153
142
29
20
20
18
13
13
11
10
6
6
5
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Options
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Catalog No. ABIN5693154
€ 247.79
Plus shipping costs €45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
20.60808 ABIN268292 ICC FACS IHC (fro) IF IHC Rat IgG2a Log in to see YTS105-18 10
19.10808 ABIN4295972 CyTOF Dep FACS ICC IF IHC IHC (fro) IHC (p) IP InhA Rat IgG2a kappa Log in to see 53-6-7 9
13.25679 ABIN2137085 FACS IHC FITC Rat IgG2a Log in to see 53-6-7 0
13.25679 ABIN289078 FACS IHC PE Rat IgG2a Log in to see 53-6-7 0
13.25679 ABIN289073 FACS IHC APC Rat IgG2a Log in to see 53-6-7 0
13.25679 ABIN289075 FACS IHC Cy5 Rat IgG2a Log in to see 53-6-7 0
13.25679 ABIN289079 FACS IHC PE Rat IgG2a Log in to see 53-6-7 0
13.25679 ABIN289081 FACS IHC PE Rat IgG2a Log in to see 53-6-7 0
13.25679 ABIN289083 FACS IHC SPRD Rat IgG2a Log in to see 53-6-7 0
13.25679 ABIN289080 FACS IHC PE-Cy7 Rat IgG2a Log in to see 53-6-7 0
13.25679 ABIN284334 FACS IP IHC Rat IgG2a Log in to see 53-6-7 0
13.25679 ABIN289074 FACS IP IHC Biotin Rat IgG2a Log in to see 53-6-7 0
13.25679 ABIN2137084 FACS IP IHC Rat IgG2a Log in to see 53-6-7 0
7 ABIN1862305 ICC IHC WB Rabbit IgG AA 27-247 Log in to see Polyclonal 0
7 ABIN2896220 IF/ICC IHC IP WB Rabbit AA 27-247 Log in to see Polyclonal 0
4 ABIN2657489 BR FACS IF IP IHC Alexa Fluor 488 Rat IgG2a kappa Log in to see 53-6-7 10
4 ABIN2658875 BR FACS IF IP IHC APC Rat IgG2a kappa Log in to see 53-6-7 10
4 ABIN475545 FACS IHC IHC (fro) Rat IgG2a, kappa Log in to see 53-6-7 0
4 ABIN3037652 FACS IF/ICC IHC IP PE Rat IgG2a Log in to see 0
4 ABIN3037653 FACS IF/ICC IHC IP APC Rat IgG2a Log in to see 0

General

Antigen CD8a Molecule (CD8A) Antibodies
Epitope AA 28-196
(81), (26), (19), (17), (15), (13), (12), (12), (12), (11), (11), (9), (9), (8), (7), (7), (6), (5), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine), Rat (Rattus)
(599), (447), (122), (91), (47), (24), (15), (15), (15), (13), (12), (9), (6), (4), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Host Rabbit
(695), (399), (261), (1)
Clonality
Polyclonal
Conjugate This CD8 antibody is un-conjugated
(144), (138), (85), (81), (33), (28), (27), (26), (21), (19), (18), (18), (17), (17), (17), (17), (17), (17), (15), (14), (14), (12), (12), (11), (11), (10), (8), (7), (6), (5), (5), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)
(1101), (429), (294), (251), (249), (209), (207), (153), (142), (29), (20), (20), (18), (13), (13), (11), (10), (6), (6), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD8 Antibody

Target Details CD8 Application Details Handling References for anti-CD8 antibody (ABIN5693154) Images
Brand Picoband™
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for CD8 alpha detection. Tested with WB, IHC-P, IHC-F, ICC, FCM, Direct ELISA in Mouse,Rat.
Immunogen E. coli-derived mouse CD8 alpha recombinant protein (Position: K28-Y196).
Plasmids, Primers & others

Target Details CD8

Product Details anti-CD8 Antibody Application Details Handling References for anti-CD8 antibody (ABIN5693154) Images back to top
Antigen
Alternative Name Cd8a (CD8A Antibody Abstract)
Background

Synonyms: T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2, CD8a, Cd8a, Lyt-2, Lyt2

Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. It is mapped to 2p11.2. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene also encodes the CD8 alpha chain isoforms.

UniProt P01731
Pathways TCR Signaling

Application Details

Product Details anti-CD8 Antibody Target Details CD8 Handling References for anti-CD8 antibody (ABIN5693154) Images back to top
Application Notes

Recommended Detection Systems: Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P), IHC(F) and ICC.

Application Details: Western blot, 0.1-0.5&mu,g/mL
Immunohistochemistry(Paraffin-embedded Section), 0.5-1&mu,g/mL
Immunohistochemistry(Frozen Section), 0.5-1&mu,g/mL
Immunocytochemistry, 0.5-1&mu,g/mL
Flow Cytometry, 1-3 μg/1x106 cells
Direct ELISA, 0.1-0.5&mu,g/mL

Restrictions For Research Use only

Handling

Product Details anti-CD8 Antibody Target Details CD8 Application Details References for anti-CD8 antibody (ABIN5693154) Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Buffer Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

References for anti-CD8 antibody (ABIN5693154)

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling Images back to top
Product cited in:

Xu, Tan, Wang, Wang, Li, Wu, Chen, Wu, Tan: "Elevated survivin expression in peripheral blood mononuclear cells is central to collateral formation in coronary chronic total occlusion." in: International journal of molecular medicine, Vol. 35, Issue 6, pp. 1501-10, 2015

Zhang, Chen, Lao, Huang: "Effect of Hejie decoction on T cell immune state of chronic hepatitis B patients." in: World journal of gastroenterology, Vol. 10, Issue 10, pp. 1436-9, 2005

Images

Product Details anti-CD8 Antibody Target Details CD8 Application Details Handling References for anti-CD8 antibody (ABIN5693154) back to top
Supplier Images
Western Blotting (WB) image for anti-CD8a Molecule (CD8A) (AA 28-196) antibody (ABIN5693154) Western blot analysis of CD8 alpha using anti-CD8 alpha antibody . Electrophoresis w...
Immunohistochemistry (IHC) image for anti-CD8a Molecule (CD8A) (AA 28-196) antibody (ABIN5693154) IHC analysis of CD8 alpha using anti-CD8 alpha antibody . CD8 alpha was detected in p...
Immunohistochemistry (IHC) image for anti-CD8a Molecule (CD8A) (AA 28-196) antibody (ABIN5693154) IHC analysis of CD8 alpha using anti-CD8 alpha antibody . CD8 alpha was detected in p...
Flow Cytometry (FACS) image for anti-CD8a Molecule (CD8A) (AA 28-196) antibody (ABIN5693154) Flow Cytometry analysis of EL-4 cells using anti-CD8 alpha antibody . Overlay histogr...