anti-Dog (Canine) CD80 antibody for Immunoprecipitation

Recommended CD80 Antibody (supplied by: Log in to see )

Antigen
CD80 Antibodies
  • B7
  • B7-1
  • B7.1
  • BB1
  • CD28LG
  • CD28LG1
  • LAB7
  • B71
  • Cd28l
  • Ly-53
  • Ly53
  • MIC17
  • TSA1
  • CD80 molecule
  • Cd80 molecule
  • CD80 antigen
  • CD80
  • Cd80
Reactivity
Mouse (Murine), Dog (Canine)
457
291
48
11
9
6
4
3
2
2
1
1
Host
Hamster
411
120
99
98
41
16
Clonality
Monoclonal
Conjugate
This CD80 antibody is un-conjugated
76
75
56
41
20
20
18
16
12
10
10
8
8
8
8
7
7
7
5
5
4
4
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
533
207
163
147
120
88
72
42
36
28
26
18
9
8
7
6
6
5
5
4
2
2
2
2
1
1
1
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Catalog No. ABIN489934
$ 189.75
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2689407 BR IHC (fro) FACS IP Armenian Hamster IgG2, kappa Log in to see 16 6
1 ABIN567985 Func IHC (fro) FACS IP Hamster IgG2 Log in to see 16-10A1 9
1 ABIN1176972 BR IHC (fro) FACS IP Armenian Hamster IgG2, kappa Log in to see 16-10A1 6
1 ABIN1821176 FACS IP IHC Hamster IgG Log in to see G1-G1-4 0
1 ABIN1967873 BR FACS Func IHC IHC (fro) IP Neut Hamster IgG2 Log in to see 16-10A1 0
1 ABIN3200026 FACS Func IHC (fro) IP Armenian Hamster IgG2 Log in to see 16-10A1 0
1 ABIN3037606 Func FACS IP IHC Hamster IgG2 Log in to see 0

General

Antigen CD80 Antibodies
Reactivity Mouse (Murine), Dog (Canine)
(457), (291), (48), (11), (9), (6), (4), (3), (2), (2), (1), (1)
Host Hamster
(411), (120), (99), (98), (41), (16)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD80 antibody is un-conjugated
(76), (75), (56), (41), (20), (20), (18), (16), (12), (10), (10), (8), (8), (8), (8), (7), (7), (7), (5), (5), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
(533), (207), (163), (147), (120), (88), (72), (42), (36), (28), (26), (18), (9), (8), (7), (6), (6), (5), (5), (4), (2), (2), (2), (2), (1), (1), (1)
Pubmed 8 references available
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Product Details anti-CD80 Antibody

Target Details CD80 Application Details Handling References for anti-CD80 antibody (ABIN489934) Images
Specificity The Armenian hamster monoclonal antibody 16-10A1 reacts with CD80 (B7-1), a 60 kDa single chain type I glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Immunogen CD80-transfected CHO cell line
Clone 16-10A1
Isotype IgG2

Target Details CD80

Product Details anti-CD80 Antibody Application Details Handling References for anti-CD80 antibody (ABIN489934) Images back to top
Antigen
Alternative Name CD80 (CD80 Antibody Abstract)
Background CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet, there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
Gene ID 12519
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process

Application Details

Product Details anti-CD80 Antibody Target Details CD80 Handling References for anti-CD80 antibody (ABIN489934) Images back to top
Application Notes Flow Cytometry: Recommended dilution: 6 µg/mL
Functional Application: Blocking

Working concentrations should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD80 Antibody Target Details CD80 Application Details References for anti-CD80 antibody (ABIN489934) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-CD80 antibody (ABIN489934)

Product Details anti-CD80 Antibody Target Details CD80 Application Details Handling Images back to top
Product cited in:

Mukherjee, Ahmed, Malu, Nandi: "Modulation of cell cycle progression by CTLA4-CD80/CD86 interactions on CD4+ T cells depends on strength of the CD3 signal: critical role for IL-2." in: Journal of leukocyte biology, Vol. 80, Issue 1, pp. 66-74, 2006

Odobasic, Kitching, Semple, Timoshanko, Tipping, Holdsworth: "Glomerular expression of CD80 and CD86 is required for leukocyte accumulation and injury in crescentic glomerulonephritis." in: Journal of the American Society of Nephrology : JASN, Vol. 16, Issue 7, pp. 2012-22, 2005

Paust, Lu, McCarty, Cantor: "Engagement of B7 on effector T cells by regulatory T cells prevents autoimmune disease." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 28, pp. 10398-403, 2004

Quah, Ni, ONeill: "In vitro hematopoiesis produces a distinct class of immature dendritic cells from spleen progenitors with limited T cell stimulation capacity." in: International immunology, Vol. 16, Issue 4, pp. 567-77, 2004

Reiser, von Gersdorff, Loos, Oh, Asanuma, Giardino, Rastaldi, Calvaresi, Watanabe, Schwarz, Faul, Kretzler, Davidson, Sugimoto, Kalluri, Sharpe, Kreidberg, Mundel: "Induction of B7-1 in podocytes is associated with nephrotic syndrome." in: The Journal of clinical investigation, Vol. 113, Issue 10, pp. 1390-7, 2004

Gueirard, Ave, Balazuc, Thiberge, Huerre, Milon, Guiso: "Bordetella bronchiseptica persists in the nasal cavities of mice and triggers early delivery of dendritic cells in the lymph nodes draining the lower and upper respiratory tract." in: Infection and immunity, Vol. 71, Issue 7, pp. 4137-43, 2003

Mencacci, Montagnoli, Bacci, Cenci, Pitzurra, Spreca, Kopf, Sharpe, Romani: "CD80+Gr-1+ myeloid cells inhibit development of antifungal Th1 immunity in mice with candidiasis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 6, pp. 3180-90, 2002

Horspool, Perrin, Woodcock, Cox, King, June, Harlan, St Louis, Lee: "Nucleic acid vaccine-induced immune responses require CD28 costimulation and are regulated by CTLA4." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 6, pp. 2706-14, 1998