anti-Human CD86 antibody for Immunoprecipitation

Recommended CD86 Antibody (supplied by: Log in to see )

Antigen
CD86 Molecule (CD86) Antibodies
  • B7-2
  • B7
  • B7.2
  • B70
  • CLS1
  • Cd28l2
  • ETC-1
  • Ly-58
  • Ly58
  • MB7
  • MB7-2
  • TS/A-2
  • CD28LG2
  • LAB72
  • CD86 molecule
  • CD86 antigen
  • CD86
  • Cd86
Reactivity
Human
457
419
180
10
6
3
2
2
1
1
Host
Mouse
385
307
110
1
Clonality
Monoclonal
Conjugate
This CD86 antibody is un-conjugated
97
94
55
47
20
14
13
11
10
9
8
7
7
6
5
5
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP), Western Blotting (WB)
610
258
177
171
133
104
90
69
54
53
42
13
13
12
11
10
8
2
2
2
2
1
1
1
1
1
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Catalog No. ABIN302084
$ 360.22
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
25.25679 ABIN302058 FACS Func IHC (f) IP WB PerCP Mouse IgG1 Log in to see BU63 9
25.25679 ABIN302014 FACS Func IHC (f) IP WB FITC Mouse IgG1 Log in to see BU63 9
25.25679 ABIN302037 FACS Func IHC (f) IP WB PE Mouse IgG1 Log in to see BU63 9
25.25679 ABIN301986 FACS Func IHC (f) IP WB APC Mouse IgG1 Log in to see BU63 9
25.25679 ABIN2749230 Func IHC (f) FACS IP WB Mouse IgG1 Log in to see BU63 9
16.25679 ABIN2144981 IHC (f) FACS IP WB PE Mouse IgG1 Log in to see 4ABU63 4
16.25679 ABIN2144984 IHC (f) FACS IP WB PE Mouse IgG1 Log in to see 4ABU63 4
16.25679 ABIN2144699 IHC (f) FACS IP WB FITC Mouse IgG1 Log in to see 4ABU63 4
16.25679 ABIN2144701 IHC (f) FACS IP WB FITC Mouse IgG1 Log in to see 4ABU63 4
13.25679 ABIN234755 IP Mouse IgG1 Log in to see BU63 0
13.25679 ABIN2137059 Func FACS IP IHC Mouse IgG2b kappa Log in to see IT2-2 0
12.900042 ABIN395963 IP Mouse IgG2a kappa AA 220-329 Log in to see 3D7 0
12.900042 ABIN560238 IP ELISA Mouse IgG2a kappa AA 220-329 Log in to see 3D7 0
10 ABIN1871657 ICC IF IHC IP WB Rabbit IgG Log in to see Polyclonal 0
10 ABIN2895521 IF/ICC IHC IP WB Rabbit AA 46-282 Log in to see Polyclonal 0
8.5 ABIN2472677 IHC (fro) FACS IP Mouse IgG1 Log in to see BU63 4
8.5 ABIN535468 FACS IF IP Mouse IgG1 Log in to see BU63 3
7 ABIN6185427 FACS IF IHC (f) IP WB PerCP Mouse IgG1, kappa Log in to see BU63 0
7 ABIN6185428 FACS IF IHC (f) IP WB PE Mouse IgG1, kappa Log in to see BU63 0
7 ABIN6185420 FACS IF IHC (f) IP WB APC Mouse IgG1, kappa Log in to see BU63 0

General

Antigen CD86 Molecule (CD86) Antibodies
Reactivity Human
(457), (419), (180), (10), (6), (3), (2), (2), (1), (1)
Host Mouse
(385), (307), (110), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD86 antibody is un-conjugated
(97), (94), (55), (47), (20), (14), (13), (11), (10), (9), (8), (7), (7), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP), Western Blotting (WB)
(610), (258), (177), (171), (133), (104), (90), (69), (54), (53), (42), (13), (13), (12), (11), (10), (8), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-CD86 Antibody

Target Details CD86 Application Details Handling References for anti-CD86 antibody (ABIN302084) Images
Specificity The antibody BU63 reacts with CD86 (B7-2), a 70 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
HLDA V, WS Code BP BP072
HLDA V, WS Code A A109
HLDA VI, WS Code BP 95
HLDA VI, WS Code B CD86.9
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Immunogen B-lymphoblastoid cell line ARH 77
Clone BU63
Isotype IgG1
Plasmids, Primers & others

Target Details CD86

Product Details anti-CD86 Antibody Application Details Handling References for anti-CD86 antibody (ABIN302084) Images back to top
Antigen
Alternative Name CD86 (CD86 Antibody Abstract)
Background CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 Molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet, there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation

Application Details

Product Details anti-CD86 Antibody Target Details CD86 Handling References for anti-CD86 antibody (ABIN302084) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD86 Antibody Target Details CD86 Application Details References for anti-CD86 antibody (ABIN302084) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References for anti-CD86 antibody (ABIN302084)

Product Details anti-CD86 Antibody Target Details CD86 Application Details Handling Images back to top
Product cited in:

Hovden, Karlsen, Jonsson, Aarstad, Appel: "Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses." in: BMC immunology, Vol. 12, pp. 2, 2011

Kolar, Mehta, Pelayo, Capra: "A novel human B cell subpopulation representing the initial germinal center population to express AID." in: Blood, Vol. 109, Issue 6, pp. 2545-52, 2007

Chan, Baird, Mercer, Fleming: "Maturation and function of human dendritic cells are inhibited by orf virus-encoded interleukin-10." in: The Journal of general virology, Vol. 87, Issue Pt 11, pp. 3177-81, 2006

Zhan, Towler, Calder: "The immunomodulatory role of human conjunctival epithelial cells." in: Investigative ophthalmology & visual science, Vol. 44, Issue 9, pp. 3906-10, 2003

Mauri, Wyss-Coray, Gallati, Pichler: "Antigen-presenting T cells induce the development of cytotoxic CD4+ T cells. I. Involvement of the CD80-CD28 adhesion molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 1, pp. 118-27, 1995

Caux, Vanbervliet, Massacrier, Azuma, Okumura, Lanier, Banchereau: "B70/B7-2 is identical to CD86 and is the major functional ligand for CD28 expressed on human dendritic cells." in: The Journal of experimental medicine, Vol. 180, Issue 5, pp. 1841-7, 1994

Engel, Gribben, Freeman, Zhou, Nozawa, Abe, Nadler, Wakasa, Tedder: "The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen." in: Blood, Vol. 84, Issue 5, pp. 1402-7, 1994

Background publications

Yadav, Judkowski, Flodstrom-Tullberg, Sterling, Redmond, Sherman, Sarvetnick: "B7-2 (CD86) controls the priming of autoreactive CD4 T cell response against pancreatic islets." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 6, pp. 3631-9, 2004

Vasilevko, Ghochikyan, Holterman, Agadjanyan: "CD80 (B7-1) and CD86 (B7-2) are functionally equivalent in the initiation and maintenance of CD4+ T-cell proliferation after activation with suboptimal doses of PHA." in: DNA and cell biology, Vol. 21, Issue 3, pp. 137-49, 2002

Images

Product Details anti-CD86 Antibody Target Details CD86 Application Details Handling References for anti-CD86 antibody (ABIN302084) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD86 Molecule (CD86) antibody (ABIN302084) Surface staining of human peripheral blood cells with anti-CD86 (BU63) PerCP (monocyt...
Flow Cytometry (FACS) image for anti-CD86 Molecule (CD86) antibody (ABIN302084) Surface staining of human peripheral blood with anti-human CD44 (MEM-85) purified / G...