anti-Human CD86 antibody for Western Blotting

Recommended CD86 Antibody (supplied by: Log in to see )

Antigen
CD86 Molecule (CD86) Antibodies
  • B7-2
  • B7
  • B7.2
  • B70
  • CLS1
  • Cd28l2
  • ETC-1
  • Ly-58
  • Ly58
  • MB7
  • MB7-2
  • TS/A-2
  • CD28LG2
  • LAB72
  • CD86 molecule
  • CD86 antigen
  • CD86
  • Cd86
Epitope
AA 23-244
15
13
13
12
9
9
7
6
5
5
4
4
4
4
4
4
3
3
2
2
2
1
1
1
1
1
Reactivity
Human
457
419
180
10
6
3
2
2
1
1
Host
Mouse
385
307
110
1
Clonality
Monoclonal
Conjugate
This CD86 antibody is un-conjugated
97
94
55
47
20
14
13
11
10
9
8
7
7
6
5
5
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Neutralization (Neut), Western Blotting (WB)
610
258
178
171
133
104
90
69
55
53
43
13
13
12
11
9
7
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN4899735
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
28.228935 ABIN302084 FACS Func IHC (f) IP WB Mouse IgG1 Log in to see BU63 9
25.78973 ABIN736701 IF (p) IHC (p) WB Rabbit IgG AA 130-180 Log in to see Polyclonal 9
25.228935 ABIN302058 FACS Func IHC (f) IP WB PerCP Mouse IgG1 Log in to see BU63 9
25.228935 ABIN302014 FACS Func IHC (f) IP WB FITC Mouse IgG1 Log in to see BU63 9
25.228935 ABIN302037 FACS Func IHC (f) IP WB PE Mouse IgG1 Log in to see BU63 9
25.228935 ABIN301986 FACS Func IHC (f) IP WB APC Mouse IgG1 Log in to see BU63 9
25.228935 ABIN2749230 Func IHC (f) FACS IP WB Mouse IgG1 Log in to see BU63 9
21.518665 ABIN969029 ELISA WB Mouse IgG1 Log in to see 1B3 3
20.018665 ABIN3021701 IHC WB Rabbit IgG Log in to see Polyclonal 0
17.728935 ABIN965821 ELISA WB Mouse IgG1 Log in to see 1B3 3
17.018665 ABIN931521 ELISA WB Mouse IgG1 Log in to see 1B3 0
16.228935 ABIN2144981 IHC (f) FACS IP WB PE Mouse IgG1 Log in to see 4ABU63 4
16.228935 ABIN2144984 IHC (f) FACS IP WB PE Mouse IgG1 Log in to see 4ABU63 4
16.228935 ABIN2144699 IHC (f) FACS IP WB FITC Mouse IgG1 Log in to see 4ABU63 4
16.228935 ABIN2144701 IHC (f) FACS IP WB FITC Mouse IgG1 Log in to see 4ABU63 4
13.228935 ABIN4901231 ELISA WB Mouse IgG1 Log in to see 1B3 0
13.228935 ABIN933701 FACS ELISA WB Biotin Mouse IgG1 Log in to see BU63 0
13.228935 ABIN2137080 ELISA WB Rabbit IgG Log in to see Polyclonal 0
13.228935 ABIN6135592 WB Rabbit Log in to see 0
13.228935 ABIN6138237 WB Rabbit Log in to see Polyclonal 0

General

Antigen CD86 Molecule (CD86) Antibodies
Epitope AA 23-244
(15), (13), (13), (12), (9), (9), (7), (6), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Reactivity Human
(457), (419), (180), (10), (6), (3), (2), (2), (1), (1)
Host Mouse
(385), (307), (110), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD86 antibody is un-conjugated
(97), (94), (55), (47), (20), (14), (13), (11), (10), (9), (8), (7), (7), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Neutralization (Neut), Western Blotting (WB)
(610), (258), (178), (171), (133), (104), (90), (69), (55), (53), (43), (13), (13), (12), (11), (9), (7), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 7 references available
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Product Details anti-CD86 Antibody

Target Details CD86 Application Details Handling References for anti-CD86 antibody (ABIN4899735) Images
Specificity Detects human B7-2/CD86 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) B7-1, recombinant mouse B7-2, recombinant rat B7-2, rhB7-H1, rhB7-H2, rhB7-H3, rhB7-H3b, rhB7-H4, or rhB7-L2 is observed.
Purification Protein A or G purified from ascites
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen S. frugiperda insect ovarian cell line Sf 21-derived recombinant human B7-2/CD86, Ala23-His244, Accession # P42081
Clone 37301
Isotype IgG1
Plasmids, Primers & others

Target Details CD86

Product Details anti-CD86 Antibody Application Details Handling References for anti-CD86 antibody (ABIN4899735) Images back to top
Antigen
Alternative Name B7-2/CD86 (CD86 Antibody Abstract)
Background Alternative Names: Activation B7-2 antigen, B70, B72, B7-2, B7-2 antigen, B-lymphocyte activation antigen B7-2, BU63, CD28 antigen ligand 2, CD28LG2B7-2 antigen), CD86, CD86 antigen, CD86 Molecule, CTLA-4 counter-receptor B7.2, FUN-1, LAB72, MGC34413, T-lymphocyte activation antigen CD86
Gene ID 942
UniProt P42081
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation

Application Details

Product Details anti-CD86 Antibody Target Details CD86 Handling References for anti-CD86 antibody (ABIN4899735) Images back to top
Application Notes Western Blot 5 μg/mL
Flow Cytometry 0.25 μg/10^6 cells
Neutralization 0.5-2.5 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-CD86 Antibody Target Details CD86 Application Details References for anti-CD86 antibody (ABIN4899735) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-CD86 antibody (ABIN4899735)

Product Details anti-CD86 Antibody Target Details CD86 Application Details Handling Images back to top
Product cited in:

Cavel, Shomron, Shabtay, Vital, Trejo-Leider, Weizman, Krelin, Fong, Wong, Amit, Gil: "Endoneurial macrophages induce perineural invasion of pancreatic cancer cells by secretion of GDNF and activation of RET tyrosine kinase receptor." in: Cancer research, Vol. 72, Issue 22, pp. 5733-43, 2012 Method employed by authors: Immunocytochemistry (ICC) (Sample species: Human).

Weissmüller, Semmler, Kalinke, Christians, Müller-Berghaus, Waibler: "ICOS-LICOS interaction is critically involved in TGN1412-mediated T-cell activation." in: Blood, Vol. 119, Issue 26, pp. 6268-77, 2012 Method employed by authors: Neutralization (Neut) (Sample species: Human).

El Fakhry, Alturaihi, Diallo, Merhi, Mourad: "Critical role of lipid rafts in CD154-mediated T cell signaling." in: European journal of immunology, Vol. 40, Issue 3, pp. 770-9, 2010 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Tso, Law, Tu, Chan, Lau: "Phagocytosis of apoptotic cells modulates mesenchymal stem cells osteogenic differentiation to enhance IL-17 and RANKL expression on CD4+ T cells." in: Stem cells (Dayton, Ohio), Vol. 28, Issue 5, pp. 939-54, 2010 Method employed by authors: Neutralization (Neut) (Sample species: Human).

van der Vliet, Wang, Yue, Koon, Balk, Exley: "Circulating myeloid dendritic cells of advanced cancer patients result in reduced activation and a biased cytokine profile in invariant NKT cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 11, pp. 7287-93, 2008 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Sakthivel, Shively, Kakoulidou, Pearce, Lefvert: "The soluble forms of CD28, CD86 and CTLA-4 constitute possible immunological markers in patients with abdominal aortic aneurysm." in: Journal of internal medicine, Vol. 261, Issue 4, pp. 399-407, 2007 Method employed by authors: ELISA (ELISA) (Sample species: Human).

Hock, Patton, Budhia, Mannari, Roberts, McKenzie: "Human plasma contains a soluble form of CD86 which is present at elevated levels in some leukaemia patients." in: Leukemia, Vol. 16, Issue 5, pp. 865-73, 2002 Method employed by authors: ELISA (ELISA) (Sample species: Human).

Images

Product Details anti-CD86 Antibody Target Details CD86 Application Details Handling References for anti-CD86 antibody (ABIN4899735) back to top
Supplier Images
Western Blotting (WB) image for anti-CD86 Molecule (CD86) (AA 23-244) antibody (ABIN4899735) Detection of Human B7-2/CD86 by Western Blot. Western blot shows lysates of Daudi hum...
Neutralization (Neut) image for anti-CD86 Molecule (CD86) (AA 23-244) antibody (ABIN4899735) Cell IL-2 Secretion Induced by B7-2/CD86 and Neutralization by Human B7-2/CD86 Antibo...
Flow Cytometry (FACS) image for anti-CD86 Molecule (CD86) (AA 23-244) antibody (ABIN4899735) Detection of B7-2/CD86 in Human Blood Monocytes by Flow Cytometry. Human peripheral b...
Western Blotting (WB) image for anti-CD86 Molecule (CD86) (AA 23-244) antibody (ABIN4899735) Western Blot Shows Human B7-2/CD86 Specificity by Using Knockout Cell Line. Western b...
Flow Cytometry (FACS) image for anti-CD86 Molecule (CD86) (AA 23-244) antibody (ABIN4899735) B7-2/CD86 Specificity is Shown by Flow Cytometry in Knockout Cell Line. B7-2/CD86 kno...
Flow Cytometry (FACS) image for anti-CD86 Molecule (CD86) (AA 23-244) antibody (ABIN4899735) Detection of B7-2/CD86 in Human Ramos Cell Line by Flow Cytometry. Human Ramos lympho...