anti-Human CD86 antibody for Western Blotting

Recommended CD86 Antibody (supplied by: Log in to see )

Antigen
CD86 Molecule (CD86) Antibodies
  • B7-2
  • B7
  • B7.2
  • B70
  • CLS1
  • Cd28l2
  • ETC-1
  • Ly-58
  • Ly58
  • MB7
  • MB7-2
  • TS/A-2
  • CD28LG2
  • LAB72
  • CD86 molecule
  • CD86 antigen
  • CD86
  • Cd86
Epitope
AA 130-180
14
13
13
12
10
9
7
6
5
5
4
4
4
4
4
4
4
3
3
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
461
412
180
10
6
3
2
2
1
1
Host
Rabbit
379
311
109
1
Clonality
Polyclonal
Conjugate
This CD86 antibody is un-conjugated
99
96
55
49
21
15
13
11
10
9
9
8
7
6
5
5
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
615
249
180
176
133
96
88
68
55
54
44
16
13
12
12
11
8
2
2
2
2
1
1
1
1
1
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Catalog No. ABIN736701
$ 318.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1357021 ABIN4899735 CyTOF FACS Neut WB Mouse IgG1 AA 23-244 Log in to see 37301 7
3.1357021 ABIN2789333 WB Rabbit C-Term Log in to see Polyclonal 0
3.1357021 ABIN3042788 FACS WB Rabbit IgG AA 24-329 Log in to see Polyclonal 0
3.1357021 ABIN2789334 WB Rabbit C-Term Log in to see Polyclonal 0
3.1357021 ABIN4880236 ELISA WB Mouse IgG1 Log in to see 1B3 0
3.1357021 ABIN6260657 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
3.1357021 ABIN969029 ELISA WB Mouse IgG1 Log in to see 1B3 3
3.1357021 ABIN5951757 FACS ICC IF IHC IHC (p) IP WB Rabbit IgG AA 1-200 Log in to see SJ20-00 0
3.1357021 ABIN1871657 ICC IF IHC IP WB Rabbit IgG Log in to see Polyclonal 0
3.1357021 ABIN1536980 WB Rabbit Ig Fraction AA 269-298, C-Term Log in to see Polyclonal 0
3.1357021 ABIN1859589 ICC IHC WB Rabbit IgG AA 46-282 Log in to see Polyclonal 0
3.1357021 ABIN2989457 IF/ICC WB Rabbit IgG Log in to see Polyclonal 0
3.1357021 ABIN1846208 ELISA WB Mouse IgG1 Log in to see 0
3.1357021 ABIN3197982 IC IF IP WB Rabbit Log in to see Polyclonal 0
3.1357021 ABIN5683356 ELISA WB Mouse IgG1 Log in to see 1B3 0
3.1357021 ABIN6043292 IF/ICC IP WB Rabbit IgG full length Log in to see Polyclonal 0
3.1357021 ABIN5574810 FACS IF IHC (p) WB Mouse IgG1 kappa Log in to see C86-1146 0
3.1357021 ABIN3021701 IHC WB Rabbit IgG Log in to see Polyclonal 0
3.1357021 ABIN3030429 ELISA WB Rabbit Ig Fraction AA 269-298 Log in to see Polyclonal 0
3.1357021 ABIN5996948 WB Rabbit IgG Log in to see 0

General

Antigen CD86 Molecule (CD86) Antibodies
Epitope AA 130-180
(14), (13), (13), (12), (10), (9), (7), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(461), (412), (180), (10), (6), (3), (2), (2), (1), (1)
Host Rabbit
(379), (311), (109), (1)
Clonality
Polyclonal
Conjugate This CD86 antibody is un-conjugated
(99), (96), (55), (49), (21), (15), (13), (11), (10), (9), (9), (8), (7), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(615), (249), (180), (176), (133), (96), (88), (68), (55), (54), (44), (16), (13), (12), (12), (11), (8), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-CD86 Antibody

Target Details CD86 Application Details Handling References for anti-CD86 antibody (ABIN736701) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from rat CD86
Isotype IgG
Plasmids, Primers & others

Target Details CD86

Product Details anti-CD86 Antibody Application Details Handling References for anti-CD86 antibody (ABIN736701) Images back to top
Antigen
Alternative Name CD86 (CD86 Antibody Abstract)
Background

This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011].

Subcellular location: Membrane bound, Intracellular

Synonyms: B7-2, Cd86 antigen, Cd86, Membrane glycoprotein

Gene ID 56822
UniProt O35531
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation

Application Details

Product Details anti-CD86 Antibody Target Details CD86 Handling References for anti-CD86 antibody (ABIN736701) Images back to top
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-CD86 Antibody Target Details CD86 Application Details References for anti-CD86 antibody (ABIN736701) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-CD86 antibody (ABIN736701)

Product Details anti-CD86 Antibody Target Details CD86 Application Details Handling Images back to top
Product cited in:

Chen, Zhang, Wang, Chen, Yan, Zhang, Zhang: "Peptide-modified chitosan hydrogels promote skin wound healing by enhancing wound angiogenesis and inhibiting inflammation." in: American journal of translational research, Vol. 9, Issue 5, pp. 2352-2362, 2017 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)). Further details: Sample: skin

Zhang, Zhang, Yin, Li, Bao, Chen: "Variation and significance of secretory immunoglobulin A, interleukin 6 and dendritic cells in oral cancer." in: Oncology letters, Vol. 13, Issue 4, pp. 2297-2303, 2017 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human). Further details: Sample: oral cancer

Han, Wang, Qin, Xia, Wu: "LPS alters the immuno-phenotype of glioma and glioma stem-like cells and induces in vivo antitumor immunity via TLR4." in: Journal of experimental & clinical cancer research : CR, Vol. 36, Issue 1, pp. 83, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Gerzanich, Makar, Guda, Kwon, Stokum, Woo, Ivanova, Ivanov, Mehta, Morris, Bryan, Bever, Simard: "Salutary effects of glibenclamide during the chronic phase of murine experimental autoimmune encephalomyelitis." in: Journal of neuroinflammation, Vol. 14, Issue 1, pp. 177, 2017 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)). Further details: Sample: spinal cord

Xie, Wu, Zhou, Yang, Tian, Huang, Meng, Li: "PICK1 confers anti-inflammatory effects in acute liver injury via suppressing M1 macrophage polarization." in: Biochimie, Vol. 127, pp. 121-32, 2016 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)). Further details: Sample: Liver

Das, Singh, Majid, Sherman, Mukhopadhyay, Wright, Martin, Dunn, Baker: "Syndesome Therapeutics for Enhancing Diabetic Wound Healing." in: Advanced healthcare materials, Vol. 5, Issue 17, pp. 2248-60, 2016 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)). Further details: Sample: skin/wound healing sample

Chen, Li, Zhao, Liang, Ma, Li, Zhu: "Prevention of ultraviolet radiation‑induced immunosuppression by sunscreen in Candida albicans‑induced delayed‑type hypersensitivity." in: Molecular medicine reports, Vol. 14, Issue 1, pp. 202-8, 2016 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)). Further details: Sample: UV?exposed dorsal skin

Ju, Xing, Yang, Zheng, Jia, Zhang, Ren: "An effective cytokine adjuvant vaccine induces autologous T-cell response against colon cancer in an animal model." in: BMC immunology, Vol. 17, Issue 1, pp. 31, 2016 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)). Further details: Sample: lymph nodes

Evans, Jonason, Bussler, Torno, Veeraraghavan, Reilly, Doherty, Seils, Winter, Mallow, Kirk, Howell, Giralico, Scrivens, Klimatcheva, Fisher, Bowers, Paris, Smith, Zauderer: "Antibody blockade of semaphorin 4D promotes immune infiltration into tumor and enhances response to other immunomodulatory therapies." in: Cancer immunology research, 2015 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)).

Images

Product Details anti-CD86 Antibody Target Details CD86 Application Details Handling References for anti-CD86 antibody (ABIN736701) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-CD86 Molecule (CD86) (AA 130-180) antibody (ABIN736701) Formalin-fixed and paraffin embedded human esophageal carcinoma tissue labeled with A...
SDS-PAGE (SDS) image for anti-CD86 Molecule (CD86) (AA 130-180) antibody (ABIN736701) L1 rat brain lysates L2 rat heart lysates probed with Anti CD86/B7-2 Polyclonal Antib...
Immunohistochemistry (IHC) image for anti-CD86 Molecule (CD86) (AA 130-180) antibody (ABIN736701) Formalin-fixed and paraffin embedded rat lung labeled with Anti-CD86/B7-2 Polyclonal ...
Western Blotting (WB) image for anti-CD86 Molecule (CD86) (AA 130-180) antibody (ABIN736701) HeLa cell lysates probed with Rabbit Anti-CD86 Polyclonal Antibody, Unconjugated at ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-CD86 Molecule (CD86) (AA 130-180) antibody (ABIN736701) Formalin-fixed and paraffin embedded human esophageal carcinoma tissue labeled with A...