anti-Mouse (Murine) CD86 antibody for Cytometry by Time of Flight

Recommended CD86 Antibody

Antigen
CD86 Molecule (CD86) Antibodies
  • B7-2
  • B7
  • B7.2
  • B70
  • CLS1
  • Cd28l2
  • ETC-1
  • Ly-58
  • Ly58
  • MB7
  • MB7-2
  • TS/A-2
  • CD28LG2
  • LAB72
  • CD86 molecule
  • CD86 antigen
  • CD86
  • Cd86
Reactivity
Mouse (Murine)
369
348
133
10
9
6
2
2
1
1
Host
Rat
304
238
127
Clonality
Monoclonal
Conjugate
This CD86 antibody is un-conjugated
89
85
46
41
14
10
9
9
8
7
6
5
5
5
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Neutralization (Neut), Western Blotting (WB)
481
220
163
137
90
82
71
55
41
28
19
13
11
8
6
3
2
2
1
1
1
Options

Available images

Catalog No. ABIN4900681
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Clonality References Details
4 ABIN2658079 CyTOF FACS Alexa Fluor 700 Rat IgG2b kappa PO3 14
4 ABIN2657856 BR CyTOF FACS IHC Alexa Fluor 647 Rat IgG2a kappa GL-1 11
1 ABIN2657857 BR CyTOF FACS IHC Alexa Fluor 647 Rat IgG2a kappa GL-1 12
-5.6575327 ABIN5683357 CyTOF FACS ICC IF IHC IHC (p) Rat IgG2 AP-MAB0803 1
-13.157533 ABIN4282744 CyTOF FACS IF IHC (p) WB Mouse IgG1 kappa C86-1146 0

General

Antigen CD86 Molecule (CD86) Antibodies
Reactivity Mouse (Murine)
(369), (348), (133), (10), (9), (6), (2), (2), (1), (1)
Host Rat
(304), (238), (127)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD86 antibody is un-conjugated
(89), (85), (46), (41), (14), (10), (9), (9), (8), (7), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Neutralization (Neut), Western Blotting (WB)
(481), (220), (163), (137), (90), (82), (71), (55), (41), (28), (19), (13), (11), (8), (6), (3), (2), (2), (1), (1), (1)
Pubmed 1 reference available
Supplier

Product Details anti-CD86 Antibody

Target Details CD86 Application Details Handling References for anti-CD86 antibody (ABIN4900681) Images
Specificity Detects mouse B7-2/CD86 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse B7-1, recombinant human (rh) B7-1 or rhB7-2 is observed.
Purification Protein A or G purified from hybridoma culture supernatant
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen LPS-activated mouse B cells
Clone GL1
Isotype IgG2a
Plasmids, Primers & others

Target Details CD86

Product Details anti-CD86 Antibody Application Details Handling References for anti-CD86 antibody (ABIN4900681) Images back to top
Antigen
Alternative Name B7-2/CD86 (CD86 Antibody Abstract)
Background Alternative Names: Activation B7-2 antigen, B70, B72, B7-2, B7-2 antigen, B-lymphocyte activation antigen B7-2, BU63, CD28 antigen ligand 2, CD28LG2B7-2 antigen), CD86, CD86 antigen, CD86 Molecule, CTLA-4 counter-receptor B7.2, FUN-1, LAB72, MGC34413, T-lymphocyte activation antigen CD86
Gene ID 942
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation

Application Details

Product Details anti-CD86 Antibody Target Details CD86 Handling References for anti-CD86 antibody (ABIN4900681) Images back to top
Application Notes Western Blot 1 μg/mL
Flow Cytometry 2.5 μg/10^6 cells
Neutralization 0.05-0.25 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-CD86 Antibody Target Details CD86 Application Details References for anti-CD86 antibody (ABIN4900681) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-CD86 antibody (ABIN4900681)

Product Details anti-CD86 Antibody Target Details CD86 Application Details Handling Images back to top
Product cited in:

Zhang, Wang, Liu, Sun, Ding, Fu, Min, Zhu, Cao: "Effective induction of immune tolerance by portal venous infusion with IL-10 gene-modified immature dendritic cells leading to prolongation of allograft survival." in: Journal of molecular medicine (Berlin, Germany), Vol. 82, Issue 4, pp. 240-9, 2004 Method employed by authors: Flow Cytometry (FACS), Flow Cytometry (FACS), Flow Cytometry (FACS) (Sample species: Mouse (Murine)).

Images

Product Details anti-CD86 Antibody Target Details CD86 Application Details Handling References for anti-CD86 antibody (ABIN4900681) back to top
Supplier Images
Neutralization (Neut) image for anti-CD86 Molecule (CD86) antibody (ABIN4900681) IL-2 secretion Induced by B7-2/CD86 and Neutralization by Mouse B7-2/CD86 Antibody. R...