anti-Mouse (Murine) CD86 antibody for Immunoprecipitation

Recommended CD86 Antibody (supplied by: Log in to see )

Antigen
CD86 Molecule (CD86) Antibodies
  • B7-2
  • B7
  • B7.2
  • B70
  • CLS1
  • Cd28l2
  • ETC-1
  • Ly-58
  • Ly58
  • MB7
  • MB7-2
  • TS/A-2
  • CD28LG2
  • LAB72
  • CD86 molecule
  • CD86 antigen
  • CD86
  • Cd86
Reactivity
Mouse (Murine)
476
437
179
10
6
4
2
2
1
1
Host
Rat
396
325
114
1
Clonality
Monoclonal
Conjugate
This CD86 antibody is conjugated to FITC
98
97
57
52
21
17
16
14
10
10
9
9
8
6
5
5
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Functional Studies (Func), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)
640
267
179
178
135
106
90
69
54
53
42
14
13
12
11
10
8
4
2
2
2
1
1
1
1
Options
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Available images

Catalog No. ABIN457341
$ 411.68
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
21.037022 ABIN457401 FACS Func ICC IHC (f) IP Rat IgG2a Log in to see GL-1 8
21.037022 ABIN457421 FACS Func ICC IHC (f) IP Rat IgG2a Log in to see GL-1 7
16.46489 ABIN2000273 ELISA IP WB Rabbit IgG AA 1-245, Extracellular Domain Log in to see Polyclonal 1
14.5 ABIN1176959 BR FM IHC (fro) FACS IP Rat IgG2a, kappa Log in to see GL1 17
14.5 ABIN2689420 BR IHC (fro) FACS IF IP Rat IgG2a, kappa Log in to see GL1 15
12.037022 ABIN284329 FACS IP IHC Rat IgG2b Log in to see 2D10 0
12.037022 ABIN289058 FACS IP IHC Biotin Rat IgG2b Log in to see 2D10 0
12.037022 ABIN289064 FACS IP IHC Biotin Rat IgG2a Log in to see GL1 0
12.037022 ABIN284328 FACS IP IHC Rat IgG2b Log in to see 2D10 0
12.037022 ABIN284330 FACS IP IHC Rat IgG2a Log in to see GL1 0
10 ABIN2909209 IF/ICC IHC IP WB Rabbit AA 37-272 Log in to see Polyclonal 0
7 ABIN6185420 FACS IF IHC (f) IP WB APC Mouse IgG1, kappa Log in to see BU63 0
7 ABIN6185427 FACS IF IHC (f) IP WB PerCP Mouse IgG1, kappa Log in to see BU63 0
7 ABIN6185428 FACS IF IHC (f) IP WB PE Mouse IgG1, kappa Log in to see BU63 0
7 ABIN6185431 FACS IF IHC (f) IP WB Mouse IgG1, kappa Log in to see BU63 0
7 ABIN3197982 IC IF IP WB Rabbit Log in to see Polyclonal 0
7 ABIN6043292 IF/ICC IP WB Rabbit IgG full length Log in to see Polyclonal 0
4 ABIN961105 FACS IHC IHC (fro) IP Neut Rat IgG2a, kappa Log in to see GL1 0
4 ABIN474873 FACS IHC IHC (fro) IP Rat IgG2a, kappa Log in to see GL1 0
4 ABIN5574817 FACS IHC (fro) IP SPRD Rat IgG2a kappa Log in to see GL1 0

General

Antigen CD86 Molecule (CD86) Antibodies
Reactivity Mouse (Murine)
(476), (437), (179), (10), (6), (4), (2), (2), (1), (1)
Host Rat
(396), (325), (114), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD86 antibody is conjugated to FITC
(98), (97), (57), (52), (21), (17), (16), (14), (10), (10), (9), (9), (8), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Functional Studies (Func), Immunocytochemistry (ICC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)
(640), (267), (179), (178), (135), (106), (90), (69), (54), (53), (42), (14), (13), (12), (11), (10), (8), (4), (2), (2), (2), (1), (1), (1), (1)
Pubmed 8 references available
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Product Details anti-CD86 Antibody

Target Details CD86 Application Details Handling References for anti-CD86 antibody (ABIN457341) Images
Specificity The rat monoclonal antibody GL-1 reacts with CD86 (B7-2), a 70-80 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
Cross-Reactivity (Details) Mouse
Immunogen LPS-activated CBA/Cs mouse splenic B cells
Clone GL-1
Isotype IgG2a
Plasmids, Primers & others

Target Details CD86

Product Details anti-CD86 Antibody Application Details Handling References for anti-CD86 antibody (ABIN457341) Images back to top
Antigen
Alternative Name CD86 (CD86 Antibody Abstract)
Background CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 Molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet, there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation

Application Details

Product Details anti-CD86 Antibody Target Details CD86 Handling References for anti-CD86 antibody (ABIN457341) Images back to top
Application Notes The reagent is designed for Flow Cytometry analysis.Suggested working concentration is 2 µ,g/mL. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
Comment

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.

Restrictions For Research Use only

Handling

Product Details anti-CD86 Antibody Target Details CD86 Application Details References for anti-CD86 antibody (ABIN457341) Images back to top
Concentration 0.5 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

References for anti-CD86 antibody (ABIN457341)

Product Details anti-CD86 Antibody Target Details CD86 Application Details Handling Images back to top
Product cited in:

Nolan, Kobayashi, Naveed, Kelly, Hoshino, Hoshino, Karulf, Rom, Weiden, Gold: "Differential role for CD80 and CD86 in the regulation of the innate immune response in murine polymicrobial sepsis." in: PLoS ONE, Vol. 4, Issue 8, pp. e6600, 2009

Radhakrishnan, Arneson, Upshaw, Howe, Felts, Colonna, Leibson, Rodriguez, Pease: "TREM-2 mediated signaling induces antigen uptake and retention in mature myeloid dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 181, Issue 11, pp. 7863-72, 2008

Edgtton, Kausman, Li, OSullivan, Lo, Hutchinson, Yagita, Holdsworth, Kitching: "Intrarenal antigens activate CD4+ cells via co-stimulatory signals from dendritic cells." in: Journal of the American Society of Nephrology : JASN, Vol. 19, Issue 3, pp. 515-26, 2008

Nolan, Weiden, Kelly, Hoshino, Hoshino, Mehta, Gold: "CD40 and CD80/86 act synergistically to regulate inflammation and mortality in polymicrobial sepsis." in: American journal of respiratory and critical care medicine, Vol. 177, Issue 3, pp. 301-8, 2008

Steptoe, Ritchie, Jones, Harrison: "Autoimmune diabetes is suppressed by transfer of proinsulin-encoding Gr-1+ myeloid progenitor cells that differentiate in vivo into resting dendritic cells." in: Diabetes, Vol. 54, Issue 2, pp. 434-42, 2005

Brasel, De Smedt, Smith, Maliszewski: "Generation of murine dendritic cells from flt3-ligand-supplemented bone marrow cultures." in: Blood, Vol. 96, Issue 9, pp. 3029-39, 2000

Benschop, Melamed, Nemazee, Cambier: "Distinct signal thresholds for the unique antigen receptor-linked gene expression programs in mature and immature B cells." in: The Journal of experimental medicine, Vol. 190, Issue 6, pp. 749-56, 1999

Hathcock, Laszlo, Dickler, Bradshaw, Linsley, Hodes: "Identification of an alternative CTLA-4 ligand costimulatory for T cell activation." in: Science (New York, N.Y.), Vol. 262, Issue 5135, pp. 905-7, 1993

Images

Product Details anti-CD86 Antibody Target Details CD86 Application Details Handling References for anti-CD86 antibody (ABIN457341) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD86 Molecule (CD86) antibody (FITC) (ABIN457341) Surface staining of PHA-activated murine splenocytes with anti-CD86 (GL-1) FITC.