anti-Mouse (Murine) CD86 antibody for Immunoprecipitation

Recommended CD86 Antibody (supplied by: Log in to see )

Antigen
CD86 Molecule (CD86) Antibodies
  • B7-2
  • B7
  • B7.2
  • B70
  • CLS1
  • Cd28l2
  • ETC-1
  • Ly-58
  • Ly58
  • MB7
  • MB7-2
  • TS/A-2
  • CD28LG2
  • LAB72
  • CD86 molecule
  • CD86 antigen
  • CD86
  • Cd86
Epitope
AA 1-245, Extracellular Domain
15
13
13
12
10
9
7
6
5
5
4
4
4
4
4
4
3
3
2
2
2
1
1
1
1
1
1
Reactivity
Mouse (Murine)
461
413
181
10
6
3
2
2
1
1
Host
Rabbit
379
311
109
1
Clonality
Polyclonal
Conjugate
This CD86 antibody is un-conjugated
99
96
55
49
21
15
13
11
10
9
9
8
7
6
5
5
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunoprecipitation (IP), Western Blotting (WB)
615
249
179
176
133
95
88
69
55
54
44
16
13
13
12
11
8
2
2
2
2
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN2000273
$ 218.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1176959 BR FM IHC (fro) FACS IP Rat IgG2a, kappa Log in to see GL1 17
1 ABIN2689420 BR IHC (fro) FACS IF IP Rat IgG2a, kappa Log in to see GL1 15
1 ABIN457341 FACS Func ICC IHC (f) IP FITC Rat IgG2a Log in to see GL-1 8
1 ABIN371015 Func FACS IHC (fro) IP Rat IgG2a Log in to see GL1 8
1 ABIN6185420 FACS IF IHC (f) IP WB APC Mouse IgG1, kappa Log in to see BU63 0
1 ABIN6185427 FACS IF IHC (f) IP WB PerCP Mouse IgG1, kappa Log in to see BU63 0
1 ABIN6185428 FACS IF IHC (f) IP WB PE Mouse IgG1, kappa Log in to see BU63 0
1 ABIN457401 FACS Func ICC IHC (f) IP Rat IgG2a Log in to see GL-1 8
1 ABIN6185431 FACS IF IHC (f) IP WB Mouse IgG1, kappa Log in to see BU63 0
1 ABIN371184 Func FACS IHC (fro) IP Rat IgG2b Log in to see 2D10 8
1 ABIN961105 FACS IHC IHC (fro) IP Neut Rat IgG2a, kappa Log in to see GL1 0
1 ABIN371185 Func FACS IHC (fro) IP Rat IgG2b Log in to see 2D10 8
1 ABIN457421 FACS Func ICC IHC (f) IP Rat IgG2a Log in to see GL-1 7
1 ABIN474873 FACS IHC IHC (fro) IP Rat IgG2a, kappa Log in to see GL1 0
1 ABIN2659881 FACS IP IHC WB PerCP Rat IgG2a kappa Log in to see GL-1 11
1 ABIN2659880 FACS IP IHC WB PerCP Rat IgG2a kappa Log in to see GL-1 11
1 ABIN4282714 FACS ICC IF IHC IHC (p) IP WB Mouse IgG1 kappa Log in to see BU63 0
1 ABIN6185404 FACS IF IHC (f) IP WB CF®555 Mouse IgG1, kappa Log in to see BU63 0
1 ABIN6185410 FACS IF IHC (f) IP WB CF®770 Mouse IgG1, kappa Log in to see BU63 0
1 ABIN6185412 FACS IF IHC (f) IP WB CF®680 Mouse IgG1, kappa Log in to see BU63 0

General

Antigen CD86 Molecule (CD86) Antibodies
Epitope AA 1-245, Extracellular Domain
(15), (13), (13), (12), (10), (9), (7), (6), (5), (5), (4), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(461), (413), (181), (10), (6), (3), (2), (2), (1), (1)
Host Rabbit
(379), (311), (109), (1)
Clonality
Polyclonal
Conjugate This CD86 antibody is un-conjugated
(99), (96), (55), (49), (21), (15), (13), (11), (10), (9), (9), (8), (7), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunoprecipitation (IP), Western Blotting (WB)
(615), (249), (179), (176), (133), (95), (88), (69), (55), (54), (44), (16), (13), (13), (12), (11), (8), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 1 reference available
Supplier Log in to see

Product Details anti-CD86 Antibody

Target Details CD86 Application Details Handling References for anti-CD86 antibody (ABIN2000273) Images
Specificity Mouse CD86 / ETC-1 / B7-2
Characteristics Produced in rabbits immunized with purified, recombinant Mouse CD86 extracellular domain (rM CD86, ABIN2007267 NP_062261.3, Met 1-Glu 245,. CD86 specific IgG was purified by mouse CD86 affinity chromatography.
Purification purified by affinity chromatography
Sterility 0.2 μm filtered
Immunogen Recombinant Mouse CD86 protein (ABIN2007267)
Isotype IgG
Plasmids, Primers & others

Target Details CD86

Product Details anti-CD86 Antibody Application Details Handling References for anti-CD86 antibody (ABIN2000273) Images back to top
Antigen
Alternative Name CD86 (CD86 Antibody Abstract)
Background CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. Recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.
NCBI Accession NP_062261.3
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation

Application Details

Product Details anti-CD86 Antibody Target Details CD86 Handling References for anti-CD86 antibody (ABIN2000273) Images back to top
Application Notes WB: 20-40 μg/mL
ELISA: 0.5-1.0 μg/mL
This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Mouse CD86. The detection limit for Mouse CD86 is 0.00245 ng/well.
IP: 1-4 μg/mg of lysate
Restrictions For Research Use only

Handling

Product Details anti-CD86 Antibody Target Details CD86 Application Details References for anti-CD86 antibody (ABIN2000273) Images back to top
Format Liquid
Buffer 0.2 μm filtered solution in PBS with 5 % trehalose
Preservative Without preservative
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C,-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Expiry Date 12 months

References for anti-CD86 antibody (ABIN2000273)

Product Details anti-CD86 Antibody Target Details CD86 Application Details Handling Images back to top
Background publications

Rau, Dieter, Luo, Priest, Baumgarth: "B7-1/2 (CD80/CD86) direct signaling to B cells enhances IgG secretion." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 12, pp. 7661-71, 2009

Images

Product Details anti-CD86 Antibody Target Details CD86 Application Details Handling References for anti-CD86 antibody (ABIN2000273) back to top
Supplier Images
Western Blotting (WB) image for anti-CD86 Molecule (CD86) (AA 1-245), (Extracellular Domain) antibody (ABIN2000273) anti-CD86 Molecule (CD86) (AA 1-245), (Extracellular Domain) antibody
Immunoprecipitation (IP) image for anti-CD86 Molecule (CD86) (AA 1-245), (Extracellular Domain) antibody (ABIN2000273) anti-CD86 Molecule (CD86) (AA 1-245), (Extracellular Domain) antibody (Image 2)