anti-Human Cytoplasmic Protein NCK1 antibody for Immunohistochemistry

Recommended Cytoplasmic Protein NCK1 Antibody (supplied by: Log in to see )

Antigen
NCK Adaptor Protein 1 (NCK1) Antibodies
  • NCK
  • NCKalpha
  • nck-1
  • NCK1
  • 6330586M15Rik
  • D230010O13Rik
  • Nck
  • Nck-1
  • Nck1
  • nck
  • nck1-a
  • nck1-b
  • nckalpha
  • NCK adaptor protein 1
  • non-catalytic region of tyrosine kinase adaptor protein 1
  • NCK adaptor protein 1b
  • NCK adaptor protein 1 L homeolog
  • NCK1
  • Nck1
  • nck1b
  • nck1.L
Reactivity
Human, Monkey
131
36
27
6
3
3
3
3
2
2
2
2
2
1
1
1
Host
Mouse
87
43
1
Clonality
Monoclonal
Conjugate
This Cytoplasmic Protein NCK1 antibody is un-conjugated
5
5
5
4
4
4
1
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
129
76
53
14
13
11
8
6
5
2
1
1
1
Options
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Catalog No. ABIN969545
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
21.992775 ABIN1845767 FACS IHC ELISA WB Mouse IgG1 Log in to see 0
18.992775 ABIN4880888 ELISA FACS IHC WB Mouse IgG1 Log in to see 5B7 0
15.755764 ABIN3021268 IHC WB Rabbit IgG Log in to see Polyclonal 0
15.755764 ABIN5663589 IF IHC WB Rabbit Log in to see Polyclonal 0
13 ABIN1873840 IHC WB Rabbit IgG Log in to see Polyclonal 0
12.992776 ABIN957751 ELISA IHC IHC (p) WB Mouse IgG1, kappa Log in to see 11D10 0
12.992776 ABIN2997496 IHC WB Rabbit IgG Log in to see Polyclonal 0
12.992776 ABIN2421903 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
12.992776 ABIN2428459 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
12.755764 ABIN2147274 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
10 ABIN1499652 ICC IF IHC IHC (p) WB Rabbit IgG AA 53-333 Log in to see Polyclonal 0
10 ABIN5014021 ICC IHC WB Rabbit IgG AA 2-377 Log in to see Polyclonal 0
10 ABIN2917490 IF/ICC IHC IP WB Rabbit AA 2-377 Log in to see Polyclonal 0
9.992776 ABIN6263537 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
9.992776 ABIN957794 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
4 ABIN1873839 IHC WB Rabbit IgG Log in to see Polyclonal 0
4 ABIN5970018 IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2887859 IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2888058 IF IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN2318044 IHC ELISA WB Rabbit IgG N-Term, AA 62-92 Log in to see Polyclonal 0

General

Antigen NCK Adaptor Protein 1 (NCK1) Antibodies
Reactivity Human, Monkey
(131), (36), (27), (6), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1)
Host Mouse
(87), (43), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Cytoplasmic Protein NCK1 antibody is un-conjugated
(5), (5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(129), (76), (53), (14), (13), (11), (8), (6), (5), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Cytoplasmic Protein NCK1 Antibody

Target Details Cytoplasmic Protein NCK1 Application Details Handling References for anti-Cytoplasmic Protein NCK1 antibody (ABIN969545) Images
Purification Purified
Immunogen Purified recombinant fragment of human NCK1 expressed in E. Coli.
Clone 5B7
Isotype IgG1
Plasmids, Primers & others

Target Details Cytoplasmic Protein NCK1

Product Details anti-Cytoplasmic Protein NCK1 Antibody Application Details Handling References for anti-Cytoplasmic Protein NCK1 antibody (ABIN969545) Images back to top
Antigen
Alternative Name NCK1 (NCK1 Antibody Abstract)
Background The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.
Molecular Weight 47 kDa
Gene ID 4690
HGNC 4690
Pathways TCR Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals

Application Details

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Handling References for anti-Cytoplasmic Protein NCK1 antibody (ABIN969545) Images back to top
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, FCM: 1:200 - 1:400
Restrictions For Research Use only

Handling

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details References for anti-Cytoplasmic Protein NCK1 antibody (ABIN969545) Images back to top
Format Liquid
Buffer PBS with 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage

References for anti-Cytoplasmic Protein NCK1 antibody (ABIN969545)

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Handling Images back to top
Product cited in:

Preisinger, Kolch: "The Bcr-Abl kinase regulates the actin cytoskeleton via a GADS/Slp-76/Nck1 adaptor protein pathway." in: Cellular signalling, Vol. 22, Issue 5, pp. 848-56, 2010

Blasutig, New, Thanabalasuriar, Dayarathna, Goudreault, Quaggin, Li, Gruenheid, Jones, Pawson: "Phosphorylated YDXV motifs and Nck SH2/SH3 adaptors act cooperatively to induce actin reorganization." in: Molecular and cellular biology, Vol. 28, Issue 6, pp. 2035-46, 2008

Images

Product Details anti-Cytoplasmic Protein NCK1 Antibody Target Details Cytoplasmic Protein NCK1 Application Details Handling References for anti-Cytoplasmic Protein NCK1 antibody (ABIN969545) back to top
Supplier Images
ELISA image for anti-NCK Adaptor Protein 1 (NCK1) antibody (ABIN969545) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-NCK Adaptor Protein 1 (NCK1) antibody (ABIN969545) Figure 2: Flow cytometric analysis of Jurkat cells using NCK1 mouse mAb (green) and n...
Immunohistochemistry (IHC) image for anti-NCK Adaptor Protein 1 (NCK1) antibody (ABIN969545) Figure 3: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues usi...
Western Blotting (WB) image for anti-NCK Adaptor Protein 1 (NCK1) antibody (ABIN969545) Figure 4: Western blot analysis using NCK1 mouse mAb against Jurkat (1), HeLa (2), HE...
Western Blotting (WB) image for anti-NCK Adaptor Protein 1 (NCK1) antibody (ABIN969545) Figure 5: Western blot analysis using NCK1 mAb against human NCK1 (AA: 203-371) recom...