anti-Human Replication Factor C (Activator 1) 3, 38kDa antibody for Western Blotting

Recommended Replication Factor C (Activator 1) 3, 38kDa Antibody (supplied by: Log in to see )

Antigen
Replication Factor C (Activator 1) 3, 38kDa (RFC3) Antibodies
  • CG5313
  • DRFC
  • DmRFC3
  • Dmel\\CG5313
  • Rfc3
  • 21.m02902
  • 2810416I22Rik
  • 38kDa
  • AU022547
  • Recc3
  • cb275
  • RFC38
  • Replication factor C subunit 3
  • replication factor C3
  • replication factor C subunit 3
  • replication factor C 38 kDa subunit
  • DNA replication factor C complex subunit Rfc3
  • replication factor C3, putative
  • replication factor C (activator 1) 3
  • replication factor C subunit 3 L homeolog
  • RfC3
  • PF14_0601
  • Chro.30359
  • PB000006.01.0
  • PC000345.04.0
  • TP04_0380
  • Tb09.211.3310
  • PY04255
  • PVX_117300
  • BBOV_IV003080
  • SJAG_02182
  • PKH_124240
  • rfc3
  • Rfc3
  • RFC3
  • rfc3.L
Epitope
C-Term
7
6
5
4
4
4
3
3
2
2
2
2
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
60
26
24
8
7
5
3
3
2
2
2
1
1
1
1
Host
Rabbit
49
10
1
Clonality
Polyclonal
Application
Western Blotting (WB)
58
22
14
10
9
7
4
3
1
Options
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Catalog No. ABIN2782474
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.08196 ABIN2782475 WB Rabbit N-Term Log in to see Polyclonal 1
3.08196 ABIN2706942 IHC WB Rabbit Center Log in to see Polyclonal 0
3.08196 ABIN389382 FACS WB Rabbit Ig Fraction AA 121-150, Center Log in to see Polyclonal 1
3.08196 ABIN650750 FACS WB Rabbit Ig Fraction AA 230-258, C-Term Log in to see Polyclonal 1
3.08196 ABIN2855682 ICC IF IHC (p) WB Rabbit IgG Center Log in to see Polyclonal 0
3.08196 ABIN394944 ELISA IP WB Mouse IgG2a kappa AA 257-356 Log in to see 1C6 0
3.08196 ABIN3186739 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal 0
3.08196 ABIN1874593 WB Rabbit IgG Log in to see Polyclonal 0
3.08196 ABIN5961885 ELISA IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
3.08196 ABIN498529 IHC (p) WB Rabbit Log in to see Polyclonal 0
3.08196 ABIN2462934 ELISA WB Rabbit Log in to see Polyclonal 0
3.08196 ABIN4350218 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
3.08196 ABIN5972234 WB Rabbit IgG Log in to see Polyclonal 0
3.08196 ABIN2468024 ELISA WB Chicken AA 1-356 Log in to see Polyclonal 0
3.08196 ABIN4350219 ICC IF IHC IHC (p) WB Rabbit IgG Center Log in to see Polyclonal 0
3.08196 ABIN519743 IF WB Mouse AA 1-356, full length Log in to see Polyclonal 0
3.08196 ABIN519744 WB Rabbit AA 1-356, full length Log in to see Polyclonal 0
3.08196 ABIN634080 WB Rabbit Log in to see Polyclonal 0
3.08196 ABIN634100 WB Rabbit Log in to see Polyclonal 0
3.08196 ABIN3001676 FACS WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen Replication Factor C (Activator 1) 3, 38kDa (RFC3) Antibodies
Epitope C-Term
(7), (6), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus), Zebrafish (Danio rerio)
(60), (26), (24), (8), (7), (5), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Host Rabbit
(49), (10), (1)
Clonality
Polyclonal
Application Western Blotting (WB)
(58), (22), (14), (10), (9), (7), (4), (3), (1)
Pubmed 1 reference available
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Product details

Target Details Application Details Handling References Images
Blocking Peptide Blocking peptide for this product available: ABIN982866
Sequence IIMKGLLSEL LHNCDGQLKG EVAQMAAYYE HRLQLGSKAI YHLEAFVAKF
Predicted Reactivity Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%
Characteristics This is a rabbit polyclonal antibody against RFC3. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the C terminal region of human RFC3

Target Details

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Antigen
Alternative Name RFC3 (RFC3 Antibody Abstract)
Background The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. RFC3 is the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described.
Alias Symbols: MGC5276, RFC38
Protein Interaction Partner: PCNA, TP53, UBC, RPA3, RPA2, RPA1, RPP40, FBXO6, ARFGAP1, VCP, RFC4, RFC5, RFC2, RFC1, PURB, PURA, MCM3, Cebpb, RAD21, HDGF, SMARCAD1, EBNA-LP, MESDC2, MYC, FAN1, MEPCE, USP20, CHTF8, RAD17, BRD4, CHTF18,
Protein Size: 356
Molecular Weight 40 kDa
Gene ID 5983
NCBI Accession NM_002915, NP_002906
UniProt P40938
Pathways Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA

Application Details

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Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 356 AA

Restrictions For Research Use only

Handling

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Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

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Product cited in:

You, Li, Kim, Kang, Koh, Chwae, Kim, Kim, Park, Jang, Kim: "Selective translational repression of truncated proteins from frameshift mutation-derived mRNAs in tumors." in: PLoS biology, Vol. 5, Issue 5, pp. e109, 2007

Images

Product details Target Details Application Details Handling References back to top
Supplier Images
Western Blotting (WB) image for anti-Replication Factor C (Activator 1) 3, 38kDa (RFC3) (C-Term) antibody (ABIN2782474) WB Suggested Anti-RFC3 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:62500 Posit...