anti-Human Replication Factor C (Activator 1) 3, 38kDa antibody for Western Blotting

Recommended Replication Factor C (Activator 1) 3, 38kDa Antibody (supplied by: Log in to see )

Antigen
Replication Factor C (Activator 1) 3, 38kDa (RFC3) Antibodies
  • CG5313
  • DRFC
  • DmRFC3
  • Dmel\\CG5313
  • Rfc3
  • 21.m02902
  • 2810416I22Rik
  • 38kDa
  • AU022547
  • Recc3
  • cb275
  • RFC38
  • Replication factor C subunit 3
  • replication factor C3
  • replication factor C subunit 3
  • replication factor C 38 kDa subunit
  • DNA replication factor C complex subunit Rfc3
  • replication factor C3, putative
  • replication factor C (activator 1) 3
  • replication factor C subunit 3 L homeolog
  • RfC3
  • PF14_0601
  • Chro.30359
  • PB000006.01.0
  • PC000345.04.0
  • TP04_0380
  • Tb09.211.3310
  • PY04255
  • PVX_117300
  • BBOV_IV003080
  • SJAG_02182
  • PKH_124240
  • rfc3
  • Rfc3
  • RFC3
  • rfc3.L
Epitope
Center
6
6
5
5
4
4
3
3
2
2
2
2
1
1
1
1
Reactivity
Human
60
27
25
9
8
5
4
3
3
3
3
2
1
1
1
Host
Rabbit
49
10
1
Clonality
Polyclonal
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
58
22
14
9
9
6
4
2
1
Options
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Catalog No. ABIN2855682
$ 466.18
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
19.55837 ABIN3018501 WB Rabbit Log in to see Polyclonal 0
16.55837 ABIN3186739 ELISA IHC WB Rabbit IgG Internal Region Log in to see Polyclonal 0
16.55837 ABIN634080 WB Rabbit Log in to see Polyclonal 0
16.55837 ABIN634100 WB Rabbit Log in to see Polyclonal 0
12.755764 ABIN5620682 IHC WB Rabbit Center Log in to see Polyclonal 0
12.755764 ABIN2150535 ELISA WB Rabbit IgG Log in to see Polyclonal 0
10.802607 ABIN394944 ELISA IP WB Mouse IgG2a kappa AA 257-356 Log in to see 1C6 0
10.802607 ABIN519743 IF WB Mouse AA 1-356, full length Log in to see Polyclonal 0
10 ABIN519745 IP ELISA WB Mouse IgG2a kappa AA 257-356, partial Log in to see 1C6 0
9.302607 ABIN2782474 WB Rabbit C-Term Log in to see Polyclonal 1
7.8026066 ABIN2706942 IHC WB Rabbit Center Log in to see Polyclonal 0
7.8026066 ABIN389382 FACS WB Rabbit Ig Fraction AA 121-150, Center Log in to see Polyclonal 1
7.8026066 ABIN650750 FACS WB Rabbit Ig Fraction AA 230-258, C-Term Log in to see Polyclonal 1
7.8026066 ABIN6264710 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
7.8026066 ABIN498529 IHC (p) WB Rabbit Log in to see Polyclonal 0
7.8026066 ABIN3001676 FACS WB Rabbit IgG Log in to see Polyclonal 0
7 ABIN5537452 FACS WB Rabbit Ig Fraction AA 121-150 Log in to see Polyclonal 0
7 ABIN5537453 FACS WB Rabbit Ig Fraction AA 230-258, C-Term Log in to see Polyclonal 0
4.8026066 ABIN2782475 WB Rabbit N-Term Log in to see Polyclonal 1
4.8026066 ABIN1874593 WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen Replication Factor C (Activator 1) 3, 38kDa (RFC3) Antibodies
Epitope Center
(6), (6), (5), (5), (4), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human
(60), (27), (25), (9), (8), (5), (4), (3), (3), (3), (3), (2), (1), (1), (1)
Host Rabbit
(49), (10), (1)
Clonality
Polyclonal
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(58), (22), (14), (9), (9), (6), (4), (2), (1)
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Product details

Target Details Application Details Handling Images
Cross-Reactivity Rhesus Monkey
Cross-Reactivity (Details) Rhesus Monkey (100 %)
Characteristics Rabbit Polyclonal antibody to RFC3 (replication factor C (activator 1) 3, 38 kDa)
RFC3 antibody
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human RFC3. The exact sequence is proprietary.
Isotype IgG

Target Details

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Antigen
Alternative Name RFC3 (RFC3 Antibody Abstract)
Background The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kDa. This gene encodes the 38 kDa subunit. This subunit is essential for the interaction between the 140 kDa subunit and the core complex that consists of the 36, 37, and 40 kDa subunits. Alternatively spliced transcript variants encoding distinct isoforms have been described.

Cellular Localization: Nucleus
Molecular Weight 41 kDa
Gene ID 5983
Pathways Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA

Application Details

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Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: HeLa

Restrictions For Research Use only

Handling

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Format Liquid
Concentration 0.43 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

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Supplier Images
Western Blotting (WB) image for anti-Replication Factor C (Activator 1) 3, 38kDa (RFC3) (Center) antibody (ABIN2855682) WB Image Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE replication factor ...
Immunofluorescence (IF) image for anti-Replication Factor C (Activator 1) 3, 38kDa (RFC3) (Center) antibody (ABIN2855682) ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RFC3, ...
Immunohistochemistry (IHC) image for anti-Replication Factor C (Activator 1) 3, 38kDa (RFC3) (Center) antibody (ABIN2855682) IHC-P Image Immunohistochemical analysis of paraffin-embedded human gastric cancer, u...