anti-Mouse (Murine) CD36 antibody for Immunofluorescence

Recommended CD36 Antibody (supplied by: Log in to see )

Antigen
CD36 Molecule (thrombospondin Receptor) (CD36) Antibodies
  • BDPLT10
  • CHDS7
  • FAT
  • GP3B
  • GP4
  • GPIV
  • PASIV
  • SCARB3
  • Fat
  • Scarb3
  • GPIIIB
  • PAS-4
  • zgc:92513
  • CD36 molecule
  • CD36 molecule (thrombospondin receptor)
  • CD36
  • Cd36
  • cd36
Epitope
AA 100-200
16
15
14
12
11
9
6
4
4
4
3
3
3
3
3
3
3
2
2
2
1
1
1
1
Reactivity
Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Primate, Rat (Rattus)
376
160
102
11
6
5
3
2
2
2
2
1
Host
Rabbit
267
147
46
12
2
Clonality
Polyclonal
Conjugate
This CD36 antibody is un-conjugated
52
27
22
20
13
9
8
6
5
5
5
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
292
161
135
100
64
46
42
41
39
29
14
3
3
3
3
3
3
2
2
2
2
1
1
1
1
Options
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Available images

Catalog No. ABIN152911
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
25.452496 ABIN2854820 ICC IF IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal 0
16.30609 ABIN2191823 Func FACS IF IP WB Mouse Log in to see CRF D-2712 4
5.5 ABIN2480130 FACS IF Rat IgG2a Log in to see MF3 4
5.5 ABIN2480134 FACS Func IF Rat IgG2a Log in to see MF3 4
5.5 ABIN2689209 Func FACS IF IP Mouse IgA, kappa Log in to see CRF D 3
4 ABIN2657411 FACS IF IP IHC Alexa Fluor 488 Hamster IgG Log in to see HM36 5
4 ABIN2657766 FACS IF IHC Alexa Fluor 647 Hamster IgG Log in to see HM36 3
4 ABIN2661064 FACS IF IHC Biotin Hamster IgG Log in to see HM36 3
1 ABIN1819918 FACS IF IP WB PE Rat IgG2a Log in to see MF3 0
1 ABIN1819916 FACS IF IP WB FITC Rat IgG2a Log in to see MF3 0
1 ABIN1819917 FACS IF IP WB Rat IgG2a Log in to see MF3 0
1 ABIN593831 FACS IF Rat IgG2a Log in to see 0
1 ABIN2881295 IF WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN1106326 Func IF IP WB Mouse IgA Log in to see CRF D2712 5
1 ABIN1821398 FACS IF IP WB Rat IgA Log in to see GF1 0
1 ABIN1819915 Func FACS IF IP WB Rat IgG2a Log in to see MF3 0
1 ABIN2657412 FACS IF IP IHC Alexa Fluor 488 Hamster IgG Log in to see HM36 5
1 ABIN2225672 FACS IF IP FITC Mouse IgA Log in to see 0
1 ABIN2225659 FACS IF IP WB Rat IgG2a Log in to see 9E27 0
1 ABIN2225660 FACS IF IP WB Rat IgG2a Log in to see 9E27 0

General

Antigen CD36 Molecule (thrombospondin Receptor) (CD36) Antibodies
Epitope AA 100-200
(16), (15), (14), (12), (11), (9), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Reactivity Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Primate, Rat (Rattus)
(376), (160), (102), (11), (6), (5), (3), (2), (2), (2), (2), (1)
Host Rabbit
(267), (147), (46), (12), (2)
Clonality
Polyclonal
Conjugate This CD36 antibody is un-conjugated
(52), (27), (22), (20), (13), (9), (8), (6), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(292), (161), (135), (100), (64), (46), (42), (41), (39), (29), (14), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1)
Pubmed 50 references available
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Product Details anti-CD36 Antibody

Target Details CD36 Application Details Handling References for anti-CD36 antibody (ABIN152911) Images
Purification Immunogen affinity purified
Immunogen A synthetic peptide mapping to a region of human CD36 between residues 100-200. [UniProt# P16671]
Plasmids, Primers & others

Target Details CD36

Product Details anti-CD36 Antibody Application Details Handling References for anti-CD36 antibody (ABIN152911) Images back to top
Antigen
Alternative Name CD36 (CD36 Antibody Abstract)
Background Gene Symbol: CD36
Molecular Weight Theoretical MW: 75 kDa
Gene ID 948
UniProt P16671
Pathways TLR Signaling, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades, Lipid Metabolism

Application Details

Product Details anti-CD36 Antibody Target Details CD36 Handling References for anti-CD36 antibody (ABIN152911) Images back to top
Application Notes Western Blot 1:500-1:2000, Immunohistochemistry 1:200-1:400, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunohistochemistry-Paraffin 1:200-1:400, Immunohistochemistry-FrozenThis CD36 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections, and Western blot analysis where a band is seen ~75-80 kDa. The theoretical molecular weight of CD36 is ~53 kDa. The difference in theoretical MW and actual MW as seen in Western blot is most likely due to the heavy glycosylation and palmitoylation of this protein. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24531551) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for CD36 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed, loading 50ug of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBST for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk, 1 % BSA in TBST for 1 hour.
. Dilute the rabbit anti-CD36 primary antibody in blocking buffer and incubate overnight at 4 degrees Celcius.
. Wash the membrane in TBST 5 times for 5 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBST 5 times for 5 minutes each.
. Apply the detection reagent of choice in accordance with the manufacturers instructions.IHC-FFPE sectionsI. Deparaffinization:A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.II. Quench Endogenous Peroxidase:A. Place slides in peroxidase quenching solution: 15-30 minutes.To Prepare 200 mL of Quenching Solution:Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol.Use within 4 hours of preparationB. Place slides in distilled water: 2 changes for 2 minutes each.III. Retrieve Epitopes:A. Preheat Citrate
Restrictions For Research Use only

Handling

Product Details anti-CD36 Antibody Target Details CD36 Application Details References for anti-CD36 antibody (ABIN152911) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-CD36 antibody (ABIN152911)

Product Details anti-CD36 Antibody Target Details CD36 Application Details Handling Images back to top
Product cited in:

Lindsey, Jung, Yabluchanskiy, Cannon, Iyer, Flynn, DeLeon-Pennell, Valerio, Harrison, Ripplinger, Hall, Ma: "Exogenous CXCL4 infusion inhibits macrophage phagocytosis by limiting CD36 signalling to enhance post-myocardial infarction cardiac dilation and mortality." in: Cardiovascular research, Vol. 115, Issue 2, pp. 395-408, 2019 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Yue, Febbraio, Klenotic, Kennedy, Wu, Chen, Gohara, Li, Belcher, Kuang, McIntyre, Silverstein, Li: "CD36 Enhances Vascular Smooth Muscle Cell Proliferation and Development of Neointimal Hyperplasia." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 39, Issue 2, pp. 263-275, 2019 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).

Zhong, Li, Zhang, Zhang, Lu, Guo, Liang, Ge, Zhu, Tao, Wu, Yin: "Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation." in: The Journal of clinical investigation, Vol. 129, Issue 1, pp. 252-267, 2019 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Lee, Han, Park, Baek, Song, Choi, Takuwa, Ryu, Kim, He, Schuchman, Bae, Jin: "Neuronal SphK1 acetylates COX2 and contributes to pathogenesis in a model of Alzheimer's Disease." in: Nature communications, Vol. 9, Issue 1, pp. 1479, 2018

Rozovski, Harris, Li, Liu, Jain, Ferrajoli, Burger, Thompson, Jain, Wierda, Keating, Estrov: "STAT3-activated CD36 facilitates fatty acid uptake in chronic lymphocytic leukemia cells." in: Oncotarget, Vol. 9, Issue 30, pp. 21268-21280, 2018

Misra, Feng, Chandran, Kabir, Rotllan, Aryal, Sheikh, Ding, Qin, Fernández-Hernando, Tellides, Greif: "Integrin beta3 regulates clonality and fate of smooth muscle-derived atherosclerotic plaque cells." in: Nature communications, Vol. 9, Issue 1, pp. 2073, 2018

Sankar, Donegan, Shah, Reddi, Wood: "Heme and hemoglobin suppress amyloid β-mediated inflammatory activation of mouse astrocytes." in: The Journal of biological chemistry, Vol. 293, Issue 29, pp. 11358-11373, 2018

Li, Lu, Ru, Lopes-Virella, Lyons, Huang: "Saturated Fatty Acid Combined with Lipopolysaccharide Stimulates a Strong Inflammatory Response in Hepatocytes in vivo and in vitro." in: American journal of physiology. Endocrinology and metabolism, 2018

Fang, Wang, Yao, Li, Wang, Liu, Tao, Sun, Liao: "The adhesion and migration of microglia to β-amyloid (Aβ) is decreased with aging and inhibited by Nogo/NgR pathway." in: Journal of neuroinflammation, Vol. 15, Issue 1, pp. 210, 2018

Lavie, Dyugovskaya, Polyakov, Rogovoy, Leder: "Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro." in: Journal of visualized experiments : JoVE, Issue 119, 2017

Chen, Huang, Yang, Xin, Cui, Xie, Silverstein: "Cardiotonic Steroids Stimulate Macrophage Inflammatory Responses Through a Pathway Involving CD36, TLR4, and Na/K-ATPase." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 37, Issue 8, pp. 1462-1469, 2017 (Sample species: Mouse (Murine)).

Xian, Ding, Dieckmann, Zhou, Plattner, Liu, Parks, Hammer, Boucher, Tsai, Herz: "LRP1 integrates murine macrophage cholesterol homeostasis and inflammatory responses in atherosclerosis." in: eLife, Vol. 6, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Guri, Colombi, Dazert, Hindupur, Roszik, Moes, Jenoe, Heim, Riezman, Riezman, Hall: "mTORC2 Promotes Tumorigenesis via Lipid Synthesis." in: Cancer cell, Vol. 32, Issue 6, pp. 807-823.e12, 2017 (Sample species: Mouse (Murine)).

Souza, Bocharov, Baranova, Vishnyakova, Huang, Wilkins, Hu, Street, Alvarez-Prats, Mullick, Patterson, Remaley, Eggerman, Yuen, Star: "Antagonism of scavenger receptor CD36 by 5A peptide prevents chronic kidney disease progression in mice independent of blood pressure regulation." in: Kidney international, Vol. 89, Issue 4, pp. 809-22, 2016 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Salameh, Daquinag, Staquicini, An, Hajjar, Pasqualini, Arap, Kolonin: "Prohibitin/annexin 2 interaction regulates fatty acid transport in adipose tissue." in: JCI insight, Vol. 1, Issue 10, 2016

Garbacz, Lu, Miller, Poloyac, Eyre, Mayrhofer, Xu, Ren, Xie: "Hepatic Overexpression of CD36 Improves Glycogen Homeostasis and Attenuates High-Fat Diet-Induced Hepatic Steatosis and Insulin Resistance." in: Molecular and cellular biology, Vol. 36, Issue 21, pp. 2715-2727, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting

Sachdev, Leopold, Bauer, Patankar, Iqbal, Obrowsky, Boverhof, Doktorova, Scheicher, Goeritzer, Kolb, Turnbull, Zimmer, Hoefler, Hussain, Groen, Kratky: "Novel role of a triglyceride-synthesizing enzyme: DGAT1 at the crossroad between triglyceride and cholesterol metabolism." in: Biochimica et biophysica acta, Vol. 1861, Issue 9 Pt A, pp. 1132-41, 2016 Method employed by authors: Western Blotting (WB)

Vaillant, Lauzier, Ruiz, Shi, Lachance, Rivard, Bolduc, Thorin, Tardif, Des Rosiers: "Ivabradine and metoprolol differentially affect cardiac glucose metabolism despite similar heart rate reduction in a mouse model of dyslipidemia." in: American journal of physiology. Heart and circulatory physiology, Vol. 311, Issue 4, pp. H991-H1003, 2016

Konstantynowicz-Nowicka, Harasim, Baranowski, Chabowski: "New evidence for the role of ceramide in the development of hepatic insulin resistance." in: PLoS ONE, Vol. 10, Issue 1, pp. e0116858, 2015 (Sample species: Rat (Rattus)). Further details: Western Blotting

Zidek, Ackermann, Hartleben, Eichwald, Kortman, Kiehntopf, Leutz, Sonenberg, Wang, von Maltzahn, Müller, Calkhoven: "Deficiency in mTORC1-controlled C/EBPβ-mRNA translation improves metabolic health in mice." in: EMBO reports, Vol. 16, Issue 8, pp. 1022-36, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting

Images

Product Details anti-CD36 Antibody Target Details CD36 Application Details Handling References for anti-CD36 antibody (ABIN152911) back to top
Supplier Images
Western Blotting (WB) image for anti-CD36 Molecule (thrombospondin Receptor) (CD36) (AA 100-200) antibody (ABIN152911) Detection of CD36 in human adipocyte extract (30 ug). Lane 1: 0.5 ug/ml ABIN152911, ...
Immunohistochemistry (IHC) image for anti-CD36 Molecule (thrombospondin Receptor) (CD36) (AA 100-200) antibody (ABIN152911) Staining of humnan adipocytes using ABIN152911.
Immunofluorescence (IF) image for anti-CD36 Molecule (thrombospondin Receptor) (CD36) (AA 100-200) antibody (ABIN152911) Immunocytochemistry/Immunofluorescence: CD36/SR-B3 Antibody [ABIN152911] - CD36 antib...
Immunofluorescence (IF) image for anti-CD36 Molecule (thrombospondin Receptor) (CD36) (AA 100-200) antibody (ABIN152911) Immunocytochemistry/Immunofluorescence: CD36/SR-B3 Antibody - Mouse liver, image is ...
Western Blotting (WB) image for anti-CD36 Molecule (thrombospondin Receptor) (CD36) (AA 100-200) antibody (ABIN152911) Western Blot: CD36/SR-B3 Antibody - Total protein from Human Skin and Adipose tissue...
Western Blotting (WB) image for anti-CD36 Molecule (thrombospondin Receptor) (CD36) (AA 100-200) antibody (ABIN152911) Western Blot: CD36/SR-B3 Antibody - Alcohol feeding significantly up-regulated CD36 ...
Western Blotting (WB) image for anti-CD36 Molecule (thrombospondin Receptor) (CD36) (AA 100-200) antibody (ABIN152911) Western Blot: CD36/SR-B3 Antibody - Mouse primary muscle cell lysate. This image was...
Western Blotting (WB) image for anti-CD36 Molecule (thrombospondin Receptor) (CD36) (AA 100-200) antibody (ABIN152911) Western Blot: CD36/SR-B3 Antibody - Alcohol feeding significantly up-regulated CD36 ...