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|Antigen||CD36 Molecule (thrombospondin Receptor) (CD36) Antibodies|
|Epitope||AA 100-200 Alternatives|
|Reactivity||Cow (Bovine), Human, Mouse (Murine), Pig (Porcine), Primate, Rat (Rattus) Alternatives|
|Conjugate||This CD36 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
|50 references available|
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Product Details anti-CD36 AntibodyTarget Details CD36 Application Details Handling References for anti-CD36 antibody (ABIN152911) Images
|Purification||Immunogen affinity purified|
|Immunogen||A synthetic peptide mapping to a region of human CD36 between residues 100-200. [UniProt# P16671]|
|Plasmids, Primers & others|
Target Details CD36Product Details anti-CD36 Antibody Application Details Handling References for anti-CD36 antibody (ABIN152911) Images back to top
|Alternative Name||CD36 (CD36 Antibody Abstract)|
|Background||Gene Symbol: CD36|
|Molecular Weight||Theoretical MW: 75 kDa|
|Pathways||TLR Signaling, Peptide Hormone Metabolism, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Lipid Metabolism by PPARalpha, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Hepatitis C, Toll-Like Receptors Cascades, Lipid Metabolism|
Application DetailsProduct Details anti-CD36 Antibody Target Details CD36 Handling References for anti-CD36 antibody (ABIN152911) Images back to top
|Application Notes||Western Blot 1:500-1:2000, Immunohistochemistry 1:200-1:400, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunohistochemistry-Paraffin 1:200-1:400, Immunohistochemistry-FrozenThis CD36 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry paraffin embedded sections, and Western blot analysis where a band is seen ~75-80 kDa. The theoretical molecular weight of CD36 is ~53 kDa. The difference in theoretical MW and actual MW as seen in Western blot is most likely due to the heavy glycosylation and palmitoylation of this protein. Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 24531551) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
Protocol specific for CD36 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed, loading 50ug of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBST for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk, 1 % BSA in TBST for 1 hour.
. Dilute the rabbit anti-CD36 primary antibody in blocking buffer and incubate overnight at 4 degrees Celcius.
. Wash the membrane in TBST 5 times for 5 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in TBST 5 times for 5 minutes each.
. Apply the detection reagent of choice in accordance with the manufacturers instructions.IHC-FFPE sectionsI. Deparaffinization:A. Treat slides with Xylene: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.B. Treat slides with 100 % Reagent Alcohol: 3 changes for 5 minutes each. Drain slides for 10 seconds between changes.II. Quench Endogenous Peroxidase:A. Place slides in peroxidase quenching solution: 15-30 minutes.To Prepare 200 mL of Quenching Solution:Add 3 mL of 30 % Hydrogen Peroxide to 200 mL of Methanol.Use within 4 hours of preparationB. Place slides in distilled water: 2 changes for 2 minutes each.III. Retrieve Epitopes:A. Preheat Citrate
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD36 Antibody Target Details CD36 Application Details References for anti-CD36 antibody (ABIN152911) Images back to top
Buffer contains: 0.02 % Sodium Azide
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Do not freeze.|
|Storage||4 °C,-20 °C|
|Storage Comment||Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.|
References for anti-CD36 antibody (ABIN152911)Product Details anti-CD36 Antibody Target Details CD36 Application Details Handling Images back to top
|Product cited in:||
Lindsey, Jung, Yabluchanskiy, Cannon, Iyer, Flynn, DeLeon-Pennell, Valerio, Harrison, Ripplinger, Hall, Ma: "Exogenous CXCL4 infusion inhibits macrophage phagocytosis by limiting CD36 signalling to enhance post-myocardial infarction cardiac dilation and mortality." in: Cardiovascular research, Vol. 115, Issue 2, pp. 395-408, 2019 Method employed by authors: Western Blotting (WB) (Sample species: Human).
Yue, Febbraio, Klenotic, Kennedy, Wu, Chen, Gohara, Li, Belcher, Kuang, McIntyre, Silverstein, Li: "CD36 Enhances Vascular Smooth Muscle Cell Proliferation and Development of Neointimal Hyperplasia." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 39, Issue 2, pp. 263-275, 2019 Method employed by authors: Immunohistochemistry (IHC) (Sample species: Mouse (Murine)).
Zhong, Li, Zhang, Zhang, Lu, Guo, Liang, Ge, Zhu, Tao, Wu, Yin: "Acetaldehyde dehydrogenase 2 interactions with LDLR and AMPK regulate foam cell formation." in: The Journal of clinical investigation, Vol. 129, Issue 1, pp. 252-267, 2019 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).
Lee, Han, Park, Baek, Song, Choi, Takuwa, Ryu, Kim, He, Schuchman, Bae, Jin: "Neuronal SphK1 acetylates COX2 and contributes to pathogenesis in a model of Alzheimer's Disease." in: Nature communications, Vol. 9, Issue 1, pp. 1479, 2018
Rozovski, Harris, Li, Liu, Jain, Ferrajoli, Burger, Thompson, Jain, Wierda, Keating, Estrov: "STAT3-activated CD36 facilitates fatty acid uptake in chronic lymphocytic leukemia cells." in: Oncotarget, Vol. 9, Issue 30, pp. 21268-21280, 2018
Misra, Feng, Chandran, Kabir, Rotllan, Aryal, Sheikh, Ding, Qin, Fernández-Hernando, Tellides, Greif: "Integrin beta3 regulates clonality and fate of smooth muscle-derived atherosclerotic plaque cells." in: Nature communications, Vol. 9, Issue 1, pp. 2073, 2018
Sankar, Donegan, Shah, Reddi, Wood: "Heme and hemoglobin suppress amyloid β-mediated inflammatory activation of mouse astrocytes." in: The Journal of biological chemistry, Vol. 293, Issue 29, pp. 11358-11373, 2018
Li, Lu, Ru, Lopes-Virella, Lyons, Huang: "Saturated Fatty Acid Combined with Lipopolysaccharide Stimulates a Strong Inflammatory Response in Hepatocytes in vivo and in vitro." in: American journal of physiology. Endocrinology and metabolism, 2018
Fang, Wang, Yao, Li, Wang, Liu, Tao, Sun, Liao: "The adhesion and migration of microglia to β-amyloid (Aβ) is decreased with aging and inhibited by Nogo/NgR pathway." in: Journal of neuroinflammation, Vol. 15, Issue 1, pp. 210, 2018
Lavie, Dyugovskaya, Polyakov, Rogovoy, Leder: "Development and Identification of a Novel Subpopulation of Human Neutrophil-derived Giant Phagocytes In Vitro." in: Journal of visualized experiments : JoVE, Issue 119, 2017
Chen, Huang, Yang, Xin, Cui, Xie, Silverstein: "Cardiotonic Steroids Stimulate Macrophage Inflammatory Responses Through a Pathway Involving CD36, TLR4, and Na/K-ATPase." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 37, Issue 8, pp. 1462-1469, 2017 (Sample species: Mouse (Murine)).
Xian, Ding, Dieckmann, Zhou, Plattner, Liu, Parks, Hammer, Boucher, Tsai, Herz: "LRP1 integrates murine macrophage cholesterol homeostasis and inflammatory responses in atherosclerosis." in: eLife, Vol. 6, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).
Guri, Colombi, Dazert, Hindupur, Roszik, Moes, Jenoe, Heim, Riezman, Riezman, Hall: "mTORC2 Promotes Tumorigenesis via Lipid Synthesis." in: Cancer cell, Vol. 32, Issue 6, pp. 807-823.e12, 2017 (Sample species: Mouse (Murine)).
Souza, Bocharov, Baranova, Vishnyakova, Huang, Wilkins, Hu, Street, Alvarez-Prats, Mullick, Patterson, Remaley, Eggerman, Yuen, Star: "Antagonism of scavenger receptor CD36 by 5A peptide prevents chronic kidney disease progression in mice independent of blood pressure regulation." in: Kidney international, Vol. 89, Issue 4, pp. 809-22, 2016 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)
Salameh, Daquinag, Staquicini, An, Hajjar, Pasqualini, Arap, Kolonin: "Prohibitin/annexin 2 interaction regulates fatty acid transport in adipose tissue." in: JCI insight, Vol. 1, Issue 10, 2016
Garbacz, Lu, Miller, Poloyac, Eyre, Mayrhofer, Xu, Ren, Xie: "Hepatic Overexpression of CD36 Improves Glycogen Homeostasis and Attenuates High-Fat Diet-Induced Hepatic Steatosis and Insulin Resistance." in: Molecular and cellular biology, Vol. 36, Issue 21, pp. 2715-2727, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting
Sachdev, Leopold, Bauer, Patankar, Iqbal, Obrowsky, Boverhof, Doktorova, Scheicher, Goeritzer, Kolb, Turnbull, Zimmer, Hoefler, Hussain, Groen, Kratky: "Novel role of a triglyceride-synthesizing enzyme: DGAT1 at the crossroad between triglyceride and cholesterol metabolism." in: Biochimica et biophysica acta, Vol. 1861, Issue 9 Pt A, pp. 1132-41, 2016 Method employed by authors: Western Blotting (WB)
Vaillant, Lauzier, Ruiz, Shi, Lachance, Rivard, Bolduc, Thorin, Tardif, Des Rosiers: "Ivabradine and metoprolol differentially affect cardiac glucose metabolism despite similar heart rate reduction in a mouse model of dyslipidemia." in: American journal of physiology. Heart and circulatory physiology, Vol. 311, Issue 4, pp. H991-H1003, 2016
Konstantynowicz-Nowicka, Harasim, Baranowski, Chabowski: "New evidence for the role of ceramide in the development of hepatic insulin resistance." in: PLoS ONE, Vol. 10, Issue 1, pp. e0116858, 2015 (Sample species: Rat (Rattus)). Further details: Western Blotting
Zidek, Ackermann, Hartleben, Eichwald, Kortman, Kiehntopf, Leutz, Sonenberg, Wang, von Maltzahn, Müller, Calkhoven: "Deficiency in mTORC1-controlled C/EBPβ-mRNA translation improves metabolic health in mice." in: EMBO reports, Vol. 16, Issue 8, pp. 1022-36, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting
ImagesProduct Details anti-CD36 Antibody Target Details CD36 Application Details Handling References for anti-CD36 antibody (ABIN152911) back to top