anti-Human Interferon Regulatory Factor 3 antibody for Immunofluorescence (Paraffin-embedded Sections)

Recommended Interferon Regulatory Factor 3 Antibody (supplied by: Log in to see )

Antigen
Interferon Regulatory Factor 3 (IRF3) Antibodies
  • irf3
  • IRF3
  • C920001K05Rik
  • IRF-3
  • interferon regulatory factor 3
  • interferon regulatory factor 7
  • irf3
  • IRF3
  • irf-3
  • Irf3
  • IRF7
Epitope
AA 375-425, pSer396
26
21
19
19
18
17
15
14
12
11
9
5
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
289
121
70
7
6
6
3
2
2
1
1
1
1
Host
Rabbit
256
63
3
Clonality
Polyclonal
Conjugate
Un-conjugated
7
6
6
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
266
129
103
77
49
41
28
25
25
14
13
8
4
3
3
2
2
1
Options
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Catalog No. ABIN742688
$ 340.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.5 ABIN682588 IF (p) IHC (p) WB Rabbit IgG AA 60-95 Log in to see Polyclonal 1
5.5 ABIN742695 IF (cc) IF (p) FITC Rabbit IgG AA 375-425, pSer396 Log in to see Polyclonal 1
1 ABIN903063 IF (p) Alexa Fluor 488 Rabbit IgG AA 60-95 Log in to see Polyclonal 0
1 ABIN903064 IF (p) Alexa Fluor 555 Rabbit IgG AA 60-95 Log in to see Polyclonal 0
1 ABIN903067 IF (p) Alexa Fluor 350 Rabbit IgG AA 60-95 Log in to see Polyclonal 0
1 ABIN903065 IF (p) Alexa Fluor 647 Rabbit IgG AA 60-95 Log in to see Polyclonal 0
1 ABIN742691 IF (cc) IF (p) Cy3 Rabbit IgG AA 375-425, pSer396 Log in to see Polyclonal 0
1 ABIN742692 IF (cc) IF (p) Cy5 Rabbit IgG AA 375-425, pSer396 Log in to see Polyclonal 0
1 ABIN742693 IF (cc) IF (p) Cy5.5 Rabbit IgG AA 375-425, pSer396 Log in to see Polyclonal 0
1 ABIN742694 IF (cc) IF (p) Cy7 Rabbit IgG AA 375-425, pSer396 Log in to see Polyclonal 0
1 ABIN682591 IF (p) Cy3 Rabbit IgG AA 60-95 Log in to see Polyclonal 0
1 ABIN682594 IF (p) Cy7 Rabbit IgG AA 60-95 Log in to see Polyclonal 0
1 ABIN682595 IF (p) FITC Rabbit IgG AA 60-95 Log in to see Polyclonal 0
1 ABIN682592 IF (p) Cy5 Rabbit IgG AA 60-95 Log in to see Polyclonal 0
1 ABIN682593 IF (p) Cy5.5 Rabbit IgG AA 60-95 Log in to see Polyclonal 0
1 ABIN875981 IF (cc) IF (p) Alexa Fluor 488 Rabbit IgG AA 375-425, pSer396 Log in to see Polyclonal 0
1 ABIN875979 IF (cc) IF (p) Alexa Fluor 647 Rabbit IgG AA 375-425, pSer396 Log in to see Polyclonal 0
1 ABIN875982 IF (cc) IF (p) Alexa Fluor 555 Rabbit IgG AA 375-425, pSer396 Log in to see Polyclonal 0
1 ABIN875980 IF (cc) IF (p) Alexa Fluor 350 Rabbit IgG AA 375-425, pSer396 Log in to see Polyclonal 0
1 ABIN2814916 IF (p) Alexa Fluor 594 Rabbit IgG AA 60-95 Log in to see Polyclonal 0

General

Antigen Interferon Regulatory Factor 3 (IRF3) Antibodies
Epitope AA 375-425, pSer396
(26), (21), (19), (19), (18), (17), (15), (14), (12), (11), (9), (5), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(289), (121), (70), (7), (6), (6), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(256), (63), (3)
Clonality
Polyclonal
Conjugate Un-conjugated
(7), (6), (6), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(266), (129), (103), (77), (49), (41), (28), (25), (25), (14), (13), (8), (4), (3), (3), (2), (2), (1)
Pubmed 4 references available
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Product details

Target Details Application Details Handling References Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic phosphopeptide derived from human IRF3 around the phosphorylation site of Ser396
Isotype IgG

Target Details

Product details Application Details Handling References Images back to top
Antigen
Alternative Name Irf3 (IRF3 Antibody Abstract)
Background

Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.

Subcellular location: Cytoplasm, Nucleus

Synonyms: Interferon regulatory factor 3, IRF-3, IRF3

Gene ID 3661
UniProt Q14653
Pathways TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades

Application Details

Product details Target Details Handling References Images back to top
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200
Restrictions For Research Use only

Handling

Product details Target Details Application Details References Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References

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Product cited in:

Ando, Matsumoto, Taguchi, Kobayashi: "Poly (I:C) impairs NO donor-induced relaxation by overexposure to NO via the NF-kappa B/iNOS pathway in rat superior mesenteric arteries." in: Free radical biology & medicine, Vol. 112, pp. 553-566, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)). Further details: Sample: arteries

Le Bel, Gosselin: "Leukotriene B4 Enhances NOD2-Dependent Innate Response against Influenza Virus Infection." in: PLoS ONE, Vol. 10, Issue 10, pp. e0139856, 2015 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)). Further details: Sample: Lung

Ichikawa, Sugiura, Koarai, Minakata, Kikuchi, Morishita, Oka, Kanai, Kawabata, Hiramatsu, Akamatsu, Hirano, Nakanishi, Matsunaga, Yamamoto, Ichinose: "TLR3 activation augments matrix metalloproteinase production through reactive nitrogen species generation in human lung fibroblasts." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 192, Issue 11, pp. 4977-88, 2014

Menasria, Boivin, Lebel, Piret, Gosselin, Boivin: "Both TRIF and IPS-1 Adaptor Proteins Contribute to the Cerebral Innate Immune Response against Herpes Simplex Virus 1 Infection." in: Journal of virology, Vol. 87, Issue 13, pp. 7301-8, 2013

Images

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Supplier Images
Immunohistochemistry (IHC) image for anti-Interferon Regulatory Factor 3 (IRF3) (AA 375-425), (pSer396) antibody (ABIN742688) Formalin-fixed and paraffin embedded mouse stomach tissue labeled with Anti-Phospho-I...
Immunofluorescence (IF) image for anti-Interferon Regulatory Factor 3 (IRF3) (AA 375-425), (pSer396) antibody (ABIN742688) Formalin-fixed and paraffin-embedded rat colitis labeled with Anti-Phospho-IRF3 (Ser3...
Western Blotting (WB) image for anti-Interferon Regulatory Factor 3 (IRF3) (AA 375-425), (pSer396) antibody (ABIN742688) Mouse liver lysates probed with Anti-IRF3 (Ser396) Polyclonal Antibody, Unconjugated ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Interferon Regulatory Factor 3 (IRF3) (AA 375-425), (pSer396) antibody (ABIN742688) Formalin-fixed and paraffin embedded mouse stomach tissue labeled with Anti-Phospho-I...
Western Blotting (WB) image for anti-Interferon Regulatory Factor 3 (IRF3) (AA 375-425), (pSer396) antibody (ABIN742688) U251 lysates probed with IRF3 (Ser396) Polyclonal Antibody, unconjugated at 1:300 ov...