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Submit your validation data for this product and get a full refund. I want to validate this productRelevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
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5.5 | ABIN1177215 | ICS | Alexa Fluor 647 | Mouse | IgG1, kappa | pSer172 | Log in to see | J133-587 | 1 | |
1 | ABIN1177214 | ICS | PE | Mouse | IgG1, kappa | pSer172 | Log in to see | J133-587 | 0 |
General |
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Antigen | TANK-Binding Kinase 1 (TBK1) Antibodies |
Epitope | pSer172 Alternatives |
Reactivity | Human, Mouse (Murine) Alternatives |
Host | Mouse Alternatives |
Clonality (Clone) | |
Conjugate | Alexa Fluor 488 Alternatives |
Application |
Intracellular Staining (ICS)
Alternatives
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1 reference available |
Supplier | Log in to see |
Product detailsTarget Details Application Details Handling References Images |
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Brand | BD Phosflow™ |
Purification | The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. |
Immunogen | Phosphorylated Human TBK1 |
Clone | J133-587 |
Isotype | IgG1, kappa |
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Antigen | |
Alternative Name | TBK1 (TBK1 Antibody Abstract) |
Background |
NF-kB is a ubiquitously expressed transcription factor that regulates many cytokine and Ig genes. It is involved in immune, inflammatory, viral, and acute phase responses. In most cells, NF-kB is sequestered in an inactive cytoplasmic form via interactions with the inhibitory proteins IkBalpha, IkBbeta, and IkBepsilon. Stimulation induces the release, activation, and nuclear translocation of NF-kB. Release of NF-kB results from the phosphorylation and proteolytic degradation of the IkB proteins. Two cytokine-inducible IkappaB kinases (IKKa and IKKb) phosphorylate and target the IkB proteins for degradation via the ubiquitin pathway. IKKg/NEMO, a third member of the IKK complex, functions as a regulatory subunit and interacts directly with IKKb. TBK1 (TANK-binding kinase 1, also known as T2K or NAK), a protein of 729 amino acids, is another member of the IKK family of kinases regulating NF-kB downstream of the tumor necrosis factor and Toll-like receptor pathways. TBK1 forms a complex with the adaptor proteins TANK (TRAF-associated NF-kB activator) and TRAF2 (TNF-receptor-associated factor 2), and this oligomer is required for activation and phosphorylation of TBK1 at serine 172 (S172). The J133-587 monoclonal antibody recognizes the phosphorylated S172 of human TBK1. Our in-house testing is performed on a cell line that has been co-transfected with TBK1 and IRF-7 because we have been unable to detect endogenous TBK1 (pS172) by western blotting of lysates from activated cell lines (HeLa activated with PMA and A431 activated with PDGF). In the transfectants, the over-expression of TBK1 is necessary for phosphorylation of IRF-7, but the presence of IRF-7 does not affect TBK1 expression or phosphorylation. We confirmed that mAb J133-587 does not cross-react with IRF-7 by ELISA. Synonyms: T2K, NAK |
Pathways | TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades |
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Sample Volume | 20 μL |
Restrictions | For Research Use only |
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Format | Liquid |
Buffer | Aqueous buffered solution containing BSA and ≤0.09 % sodium azide. |
Preservative | Sodium azide |
Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
Storage | 4 °C |
Storage Comment | Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed. |
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Product cited in: |
Viatour, Merville, Bours, Chariot: "Phosphorylation of NF-kappaB and IkappaB proteins: implications in cancer and inflammation." in: Trends in biochemical sciences, Vol. 30, Issue 1, pp. 43-52, 2005 |