anti-Mouse (Murine) TANK-Binding Kinase 1 antibody for Intracellular Staining

Recommended TANK-Binding Kinase 1 Antibody (supplied by: Log in to see )

Antigen
TANK-Binding Kinase 1 (TBK1) Antibodies
  • nak
  • t2k
  • TBK1
  • wu:fk70c05
  • zgc:136548
  • NAK
  • T2K
  • 1200008B05Rik
  • AI462036
  • AW048562
  • TANK binding kinase 1 L homeolog
  • TANK binding kinase 1
  • TANK-binding kinase 1
  • tbk1.L
  • TBK1
  • tbk1
  • Tbk1
Epitope
pSer172
19
18
17
11
7
7
5
4
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
134
83
48
27
25
9
9
8
7
5
4
3
2
1
1
1
1
1
Host
Mouse
107
34
13
Clonality
Monoclonal
Conjugate
Alexa Fluor 488
10
10
9
7
5
5
3
2
1
1
1
1
1
1
1
1
1
Application
Intracellular Staining (ICS)
136
71
62
31
24
19
9
5
3
2
2
1
Options
Supplier
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Supplier Product No.
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Pubmed
1 reference available
Catalog No. ABIN1177216
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
5.5 ABIN1177215 ICS Alexa Fluor 647 Mouse IgG1, kappa pSer172 Log in to see J133-587 1
1 ABIN1177214 ICS PE Mouse IgG1, kappa pSer172 Log in to see J133-587 0

General

Antigen TANK-Binding Kinase 1 (TBK1) Antibodies
Epitope pSer172
(19), (18), (17), (11), (7), (7), (5), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(134), (83), (48), (27), (25), (9), (9), (8), (7), (5), (4), (3), (2), (1), (1), (1), (1), (1)
Host Mouse
(107), (34), (13)
Clonality (Clone)
Monoclonal   ( )
Conjugate Alexa Fluor 488
(10), (10), (9), (7), (5), (5), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Intracellular Staining (ICS)
(136), (71), (62), (31), (24), (19), (9), (5), (3), (2), (2), (1)
Pubmed 1 reference available
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Product details

Target Details Application Details Handling References Images
Brand BD Phosflow™
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Phosphorylated Human TBK1
Clone J133-587
Isotype IgG1, kappa

Target Details

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Antigen
Alternative Name TBK1 (TBK1 Antibody Abstract)
Background NF-kB is a ubiquitously expressed transcription factor that regulates many cytokine and Ig genes. It is involved in immune, inflammatory, viral, and acute phase responses. In most cells, NF-kB is sequestered in an inactive cytoplasmic form via interactions with the inhibitory proteins IkBalpha, IkBbeta, and IkBepsilon. Stimulation induces the release, activation, and nuclear translocation of NF-kB. Release of NF-kB results from the phosphorylation and proteolytic degradation of the IkB proteins. Two cytokine-inducible IkappaB kinases (IKKa and IKKb) phosphorylate and target the IkB proteins for degradation via the ubiquitin pathway. IKKg/NEMO, a third member of the IKK complex, functions as a regulatory subunit and interacts directly with IKKb. TBK1 (TANK-binding kinase 1, also known as T2K or NAK), a protein of 729 amino acids, is another member of the IKK family of kinases regulating NF-kB downstream of the tumor necrosis factor and Toll-like receptor pathways. TBK1 forms a complex with the adaptor proteins TANK (TRAF-associated NF-kB activator) and TRAF2 (TNF-receptor-associated factor 2), and this oligomer is required for activation and phosphorylation of TBK1 at serine 172 (S172). The J133-587 monoclonal antibody recognizes the phosphorylated S172 of human TBK1. Our in-house testing is performed on a cell line that has been co-transfected with TBK1 and IRF-7 because we have been unable to detect endogenous TBK1 (pS172) by western blotting of lysates from activated cell lines (HeLa activated with PMA and A431 activated with PDGF). In the transfectants, the over-expression of TBK1 is necessary for phosphorylation of IRF-7, but the presence of IRF-7 does not affect TBK1 expression or phosphorylation. We confirmed that mAb J133-587 does not cross-react with IRF-7 by ELISA.
Synonyms: T2K, NAK
Pathways TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades

Application Details

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Sample Volume 20 μL
Restrictions For Research Use only

Handling

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Format Liquid
Buffer Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 488 under optimum conditions, and unreacted Alexa Fluor® 488 was removed.

References

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Product cited in:

Viatour, Merville, Bours, Chariot: "Phosphorylation of NF-kappaB and IkappaB proteins: implications in cancer and inflammation." in: Trends in biochemical sciences, Vol. 30, Issue 1, pp. 43-52, 2005