anti-Mouse (Murine) TLR3 antibody for Immunohistochemistry (Frozen Sections)

Recommended TLR3 Antibody (supplied by: Log in to see )

Antigen
Toll-Like Receptor 3 (TLR3) Antibodies
  • CD283
  • IIAE2
  • AI957183
  • zgc:123006
  • toll like receptor 3
  • toll-like receptor 3
  • TLR3
  • Tlr3
  • tlr3
Reactivity
Human, Mouse (Murine), Pig (Porcine)
280
206
64
45
4
4
3
2
2
2
1
1
1
1
1
Host
Rabbit
200
123
39
3
2
Clonality
Polyclonal
Conjugate
This TLR3 antibody is un-conjugated
34
30
24
16
10
8
7
6
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
239
143
104
90
67
44
40
37
13
12
12
7
5
4
1
1
1
Supplier
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Catalog No. ABIN252527
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.5 ABIN2479740 IHC (fro) WB Rabbit IgG C-Term Log in to see Polyclonal 3
7 ABIN208623 FACS IHC IHC (fro) WB Rabbit IgG AA 135-150 Log in to see Polyclonal 0
4 ABIN265140 IHC (fro) WB Rabbit IgG N-Term Log in to see Polyclonal 1
1 ABIN1106214 Func IHC (fro) IF IHC (p) IP WB FITC Mouse IgG1 Log in to see TLR3-7 4
1 ABIN1106212 Func IHC (fro) FACS IF IHC (p) IP WB Mouse IgG1 Log in to see TLR3-7 5
1 ABIN1106213 Func IHC (fro) IF IHC (p) IP WB Biotin Mouse IgG1 Log in to see TLR3-7 4
-3.7582502 ABIN4360085 ICC IF IHC (fro) WB Rabbit pTyr759 Log in to see Polyclonal 6
-12.75825 ABIN4360070 FACS IHC IHC (fro) IHC (p) WB Rabbit Log in to see Polyclonal 0

General

Antigen Toll-Like Receptor 3 (TLR3) Antibodies
Reactivity Human, Mouse (Murine), Pig (Porcine)
(280), (206), (64), (45), (4), (4), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Host Rabbit
(200), (123), (39), (3), (2)
Clonality
Polyclonal
Conjugate This TLR3 antibody is un-conjugated
(34), (30), (24), (16), (10), (8), (7), (6), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(239), (143), (104), (90), (67), (44), (40), (37), (13), (12), (12), (7), (5), (4), (1), (1), (1)
Pubmed 14 references available
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Product Details anti-TLR3 Antibody

Target Details TLR3 Application Details Handling References for anti-TLR3 antibody (ABIN252527) Images
Purification Protein G purified
Immunogen A mix of synthetic peptides corresponding to amino acids 135-150 (SIHKIKSNPFKNQKNL), 828-844 (CRRFKVHHAVQQAIEQN), and 876-891 (CILNWPVQKERINAFH) of mouse TLR3.
Plasmids, Primers & others

Target Details TLR3

Product Details anti-TLR3 Antibody Application Details Handling References for anti-TLR3 antibody (ABIN252527) Images back to top
Antigen
Alternative Name TLR3 (TLR3 Antibody Abstract)
Background Gene Symbol: TLR3
Molecular Weight Theoretical MW: 104 kDa
Gene ID 7098
UniProt O15455
Pathways TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades

Application Details

Product Details anti-TLR3 Antibody Target Details TLR3 Handling References for anti-TLR3 antibody (ABIN252527) Images back to top
Application Notes Western Blot 1-3 μg/mL, Flow Cytometry 1 μg/10^6 cells, Immunohistochemistry 1:20-1:1000, Immunohistochemistry-Paraffin 1:100, Immunohistochemistry-Frozen 1:20-1:1000Flow Cytometry (Cell Surface): see Pawar et al. (2006) for details Flow Cytometry (Intracellular): 1 μg/10^6 cells Immunohistochemistry-Frozen: see Pwar et al, 2005 for details. Immunohistochemistry-Paraffin: 1:50 (see Patol et al, 2005 for details) The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-TLR3 Antibody Target Details TLR3 Application Details References for anti-TLR3 antibody (ABIN252527) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-TLR3 antibody (ABIN252527)

Product Details anti-TLR3 Antibody Target Details TLR3 Application Details Handling Images back to top
Product cited in:

Jeon, Kim, Koh, Chung, Ha: "Pellino-1 confers chemoresistance in lung cancer cells by upregulating cIAP2 through Lys63-mediated polyubiquitination." in: Oncotarget, Vol. 7, Issue 27, pp. 41811-41824, 2016 Method employed by authors: Dot Blot (DB) (Sample species: Human).

Jiang, Broering, Trippler, Wu, Zhang, Zhang, Gerken, Lu, Schlaak: "MicroRNA-155 controls Toll-like receptor 3- and hepatitis C virus-induced immune responses in the liver." in: Journal of viral hepatitis, Vol. 21, Issue 2, pp. 99-110, 2014 (Sample species: Mouse (Murine)). Further details: Cell Sorting

Priya, Dhanwani, Patro, Rao, Parida: "Differential regulation of TLR mediated innate immune response of mouse neuronal cells following infection with novel ECSA genotype of Chikungunya virus with and without E1:A226V mutation." in: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases, Vol. 20, pp. 396-406, 2013 (Sample species: Mouse (Murine)). Further details: Western Blotting

Yamashita, Chattopadhyay, Fensterl, Saikia, Wetzel, Sen: "Epidermal growth factor receptor is essential for Toll-like receptor 3 signaling." in: Science signaling, Vol. 5, Issue 233, pp. ra50, 2012

Kleinman, Kaneko, Cho, Dridi, Fowler, Blandford, Albuquerque, Hirano, Terasaki, Kondo, Fujita, Ambati, Tarallo, Gelfand, Bogdanovich, Baffi, Ambati: "Short-interfering RNAs induce retinal degeneration via TLR3 and IRF3." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 20, Issue 1, pp. 101-8, 2012

Desai, Gong, Chan, Davey, Soong, Kolokoltsov, Sun: "Differential, type I interferon-mediated autophagic trafficking of hepatitis C virus proteins in mouse liver." in: Gastroenterology, Vol. 141, Issue 2, pp. 674-85, 685.e1-6, 2011

Wu, Meng, Jiang, Zhang, Trippler, Broering, Bucchi, Krux, Dittmer, Yang, Roggendorf, Gerken, Lu, Schlaak: "Toll-like receptor-induced innate immune responses in non-parenchymal liver cells are cell type-specific." in: Immunology, Vol. 129, Issue 3, pp. 363-74, 2010

Cho, Albuquerque, Kleinman, Tarallo, Greco, Nozaki, Green, Baffi, Ambati, De Falco, Alexander, Brunetti, De Falco, Ambati: "Small interfering RNA-induced TLR3 activation inhibits blood and lymphatic vessel growth." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 106, Issue 17, pp. 7137-42, 2009

Kleinman, Yamada, Takeda, Chandrasekaran, Nozaki, Baffi, Albuquerque, Yamasaki, Itaya, Pan, Appukuttan, Gibbs, Yang, Karikó, Ambati, Wilgus, DiPietro, Sakurai, Zhang, Smith, Taylor, Ambati: "Sequence- and target-independent angiogenesis suppression by siRNA via TLR3." in: Nature, Vol. 452, Issue 7187, pp. 591-7, 2008

Pawar, Patole, Ellwart, Lech, Segerer, Schlondorff, Anders: "Ligands to nucleic acid-specific toll-like receptors and the onset of lupus nephritis." in: Journal of the American Society of Nephrology : JASN, Vol. 17, Issue 12, pp. 3365-73, 2006

Patole, Pawar, Lech, Zecher, Schmidt, Segerer, Ellwart, Henger, Kretzler, Anders: "Expression and regulation of Toll-like receptors in lupus-like immune complex glomerulonephritis of MRL-Fas(lpr) mice." in: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, Vol. 21, Issue 11, pp. 3062-73, 2006 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Frozen Sections)

Patole, Gröne, Segerer, Ciubar, Belemezova, Henger, Kretzler, Schlöndorff, Anders: "Viral double-stranded RNA aggravates lupus nephritis through Toll-like receptor 3 on glomerular mesangial cells and antigen-presenting cells." in: Journal of the American Society of Nephrology : JASN, Vol. 16, Issue 5, pp. 1326-38, 2005 Further details: Cell Sorting,Flow Cytometry,Immunochromatography

Pawar, Patole, Zecher, Segerer, Kretzler, Schlöndorff, Anders: "Toll-like receptor-7 modulates immune complex glomerulonephritis." in: Journal of the American Society of Nephrology : JASN, Vol. 17, Issue 1, pp. 141-9, 2005 Further details: Cell Sorting,Flow Cytometry,Immunochromatography

Gao, Zhang, Wang, Liu, Wang, Cao, Hu, Yuan, Lin: "TLR3 contributes to persistent autophagy and heart failure in mice after myocardial infarction." in: Journal of cellular and molecular medicine, Vol. 22, Issue 1, pp. 395-408, 1970 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Images

Product Details anti-TLR3 Antibody Target Details TLR3 Application Details Handling References for anti-TLR3 antibody (ABIN252527) back to top
Supplier Images
Western Blotting (WB) image for anti-Toll-Like Receptor 3 (TLR3) antibody (ABIN252527) Western Blot: TLR3 Antibody [NB100-55823] - Western blot analysis of TLR3 in 15 ug/la...
Immunohistochemistry (IHC) image for anti-Toll-Like Receptor 3 (TLR3) antibody (ABIN252527) Immunohistochemistry: TLR3 Antibody [ABIN2525271] - Pig lung poisoned through aspirat...
Flow Cytometry (FACS) image for anti-Toll-Like Receptor 3 (TLR3) antibody (ABIN252527) Flow Cytometry: TLR3 Antibody [ABIN2525271] - Analysis using the FITC conjugate of AB...
Flow Cytometry (FACS) image for anti-Toll-Like Receptor 3 (TLR3) antibody (ABIN252527) Flow Cytometry: TLR3 Antibody [ABIN2525271] - Intracellular flow analysis of TLR3 in ...
Immunohistochemistry (Frozen Sections) (IHC (fro)) image for anti-Toll-Like Receptor 3 (TLR3) antibody (ABIN252527) Immunohistochemistry-Frozen: TLR3 Antibody [ABIN2525271] - Frozen mouse eye tissue se...