anti-Mouse (Murine) Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 antibody for Flow Cytometry

Recommended Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 Antibody (supplied by: Log in to see )

Antigen
Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1) Antibodies
  • FPN1
  • slc39a1
  • weh
  • slc40a1
  • MGC82700
  • SLC40A1
  • ARABIDOPSIS IRON-REGULATED TRANSPORTER 1
  • ATIRT1
  • T16H5.50
  • T16H5_50
  • iron-regulated transporter 1
  • A. THALIANA CATION DIFFUSION FACILITATOR 1
  • ATCDF1
  • ATMTP1
  • F19D11.8
  • MTP1
  • ZAT1
  • ZINC TRANSPORTER OF ARABIDOPSIS THALIANA 1
  • ZINC TRANSPORTER ZAT
  • zinc transporter of Arabidopsis thaliana
  • HFE4
  • IREG1
  • MST079
  • SLC11A3
  • Dusg
  • Fpn1
  • MTP
  • Ol5
  • Pcm
  • Slc11a3
  • Slc39a1
  • ARABIDOPSIS THALIANA IRON-REGULATED PROTEIN 1
  • ATIREG1
  • FERROPORTIN 1
  • T19C21.5
  • T19C21_5
  • iron regulated 1
  • solute carrier family 40 (iron-regulated transporter), member 1
  • Fe(2+) transport protein 1
  • metal tolerance protein 1 short form
  • solute carrier family 40 member 1
  • slc40a1
  • SLC40A1
  • IRT1
  • ZAT
  • Slc40a1
  • IREG1
Epitope
AA 250-300, Internal Region
16
13
9
6
3
3
2
2
2
1
1
Reactivity
Human, Mouse (Murine)
84
40
19
4
3
2
2
2
1
1
1
Host
Rabbit
59
24
4
Clonality
Polyclonal
Conjugate
Un-conjugated
6
4
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
67
42
38
30
18
13
13
11
1
1
1
1
Supplier
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Catalog No. ABIN439384
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.996978 ABIN4311374 FACS PE Rabbit AA 250-300, Internal Region Log in to see Polyclonal 1
13.996978 ABIN4311377 FACS ICC IF IHC IHC (p) WB Alexa Fluor 647 Rabbit AA 250-300, Internal Region Log in to see Polyclonal
13.996978 ABIN4311378 FACS ICC IF IHC IHC (p) WB Alexa Fluor 700 Rabbit AA 250-300, Internal Region Log in to see Polyclonal
1 ABIN4271947 FACS ICC IF IHC IHC (p) WB DyLight 550 Rabbit AA 250-300, Internal Region Log in to see Polyclonal
1 ABIN4271946 FACS ICC IF IHC IHC (p) WB DyLight 488 Rabbit AA 250-300, Internal Region Log in to see Polyclonal
1 ABIN4271948 FACS ICC IF IHC IHC (p) WB DyLight 650 Rabbit AA 250-300, Internal Region Log in to see Polyclonal
1 ABIN4311375 FACS ICC IF IHC IHC (p) WB Alexa Fluor 405 Rabbit AA 250-300, Internal Region Log in to see Polyclonal
1 ABIN4311376 FACS ICC IF IHC IHC (p) WB Alexa Fluor 488 Rabbit AA 250-300, Internal Region Log in to see Polyclonal
1 ABIN4311373 FACS ICC IF IHC IHC (p) WB DyLight 405 Rabbit AA 250-300, Internal Region Log in to see Polyclonal
1 ABIN4271949 FACS ICC IF IHC IHC (p) FITC Rabbit AA 250-300, Internal Region Log in to see Polyclonal

General

Antigen Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1) Antibodies
Epitope AA 250-300, Internal Region
(16), (13), (9), (6), (3), (3), (2), (2), (2), (1), (1)
Reactivity Human, Mouse (Murine)
(84), (40), (19), (4), (3), (2), (2), (2), (1), (1), (1)
Host Rabbit
(59), (24), (4)
Clonality
Polyclonal
Conjugate Un-conjugated
(6), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(67), (42), (38), (30), (18), (13), (13), (11), (1), (1), (1), (1)
Pubmed 20 references available
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Product details

Target details Application Details Handling References Images
Purification Immunogen affinity purified
Immunogen Synthetic peptide made to an internal portion of human Ferroportin 1 (within residues 250-300). [Swiss-Prot# Q9NP59]

Target details

Product details Application Details Handling References Images back to top
Antigen
Alternative Name Ferroportin/SLC40A1 (SLC40A1 Antibody Abstract)
Background Gene Symbol: SLC40A1
Molecular Weight Theoretical MW: 62 kDa
Gene ID 30061
UniProt Q9NP59
Pathways Transition Metal Ion Homeostasis

Application Details

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Application Notes Western Blot 1.0 μg/mL, Flow Cytometry 1:10, Immunohistochemistry 1:200, Immunocytochemistry/Immunofluorescence 1:500, Immunohistochemistry-Paraffin 1:200This Ferroportin 1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~62 kDa.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Protocol Protocol specific for SLC40A1 Antibody Procedure Guide for NBP1-21502 - SLC40A1 AntibodyWestern Blot Protocol
1. Perform SDS-PAGE (4-12 % MOPS) on samples to be analyzed, loading 30 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transferapparatus.
. Rinse membrane with dH2O and then stain the blot using Ponceau S for 1-2 minutes to access the transfer ofproteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locationsand locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % NFDM + 1 % BSA in TBS + Tween, 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10minutes each.
. Dilute the rabbit anti-SLC40A1 primary antibody in blocking buffer and incubate 1 hour at roomtemperature.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturersinstructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated asrequired to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions (Pierce ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, providedit does not interfere with antibody-antigen binding.
Restrictions For Research Use only

Handling

Product details Target details Application Details References Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References

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Product cited in:

Fader, Nault, Kirby, Markous, Matthews, Zacharewski: "Convergence of hepcidin deficiency, systemic iron overloading, heme accumulation, and REV-ERBα/β activation in aryl hydrocarbon receptor-elicited hepatotoxicity." in: Toxicology and applied pharmacology, Vol. 321, pp. 1-17, 2017

Marques, Porto, Rêma, Faria, Cruz Paula, Gomez-Lazaro, Silva, Martins da Silva, Lopes: "Local iron homeostasis in the breast ductal carcinoma microenvironment." in: BMC cancer, Vol. 16, pp. 187, 2016 (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Theurl, Song, Clark, Sterling, Grieco, Altamura, Galy, Hentze, Muckenthaler, Dunaief: "Mice with hepcidin-resistant ferroportin accumulate iron in the retina." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 30, Issue 2, pp. 813-23, 2016 Method employed by authors: Immunohistochemistry (IHC)

Zhang, Li, Guo, Jiang, Ma, Ke, Qian: "(Z)-ligustilide increases ferroportin1 expression and ferritin content in ischemic SH-SY5Y cells." in: European journal of pharmacology, Vol. 792, pp. 48-53, 2016

Mertens, Akam, Rehwald, Brüne, Tomat, Jung: "Intracellular Iron Chelation Modulates the Macrophage Iron Phenotype with Consequences on Tumor Progression." in: PLoS ONE, Vol. 11, Issue 11, pp. e0166164, 2016 (Sample species: Mouse (Murine)).

Gasperini, Meneghetti, Legname, Benetti: "In Absence of the Cellular Prion Protein, Alterations in Copper Metabolism and Copper-Dependent Oxidase Activity Affect Iron Distribution." in: Frontiers in neuroscience, Vol. 10, pp. 437, 2016 Method employed by authors: Western Blotting (WB) (Sample species: Rat (Rattus)).

Kim, Kim, Jung, Kim, Jeong, Lee, Yuk, Oh, Choy, Dooley, Muckenthaler, Lee, Choi: "Orphan nuclear receptor SHP regulates iron metabolism through inhibition of BMP6-mediated hepcidin expression." in: Scientific reports, Vol. 6, pp. 34630, 2016 Method employed by authors: Western Blotting (WB)

Asperti, Stuemler, Poli, Gryzik, Lifshitz, Meyron-Holtz, Vlodavsky, Arosio: "Heparanase Overexpression Reduces Hepcidin Expression, Affects Iron Homeostasis and Alters the Response to Inflammation." in: PLoS ONE, Vol. 11, Issue 10, pp. e0164183, 2016 Method employed by authors: Western Blotting (WB)

Jeong, Lee, Finley, Schmidt, Fleming, Haigis: "SIRT3 regulates cellular iron metabolism and cancer growth by repressing iron regulatory protein 1." in: Oncogene, Vol. 34, Issue 16, pp. 2115-24, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting

Katz, Reifen, Lerner: "β-Carotene can reverse dysregulation of iron protein in an in vitro model of inflammation." in: Immunologic research, Vol. 61, Issue 1-2, pp. 70-8, 2015 (Sample species: Human). Further details: Western Blotting

Gammella, Diaz, Recalcati, Buratti, Samaja, Dey, Noguchi, Gassmann, Cairo: "Erythropoietin's inhibiting impact on hepcidin expression occurs indirectly." in: American journal of physiology. Regulatory, integrative and comparative physiology, Vol. 308, Issue 4, pp. R330-5, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting

Bose, Megyesi, Shah, Hiatt, Hall, Karaduta, Swaminathan: "Evidence Suggesting a Role of Iron in a Mouse Model of Nephrogenic Systemic Fibrosis." in: PLoS ONE, Vol. 10, Issue 8, pp. e0136563, 2015 (Sample species: Mouse (Murine)). Further details: Western Blotting

Mori, Ito, Shi, Wang, Ishida, Hattori, Niwa, Hirayama, Nagasawa, Iwase, Kikkawa, Toyokuni: "Ovarian endometriosis-associated stromal cells reveal persistently high affinity for iron." in: Redox biology, Vol. 6, pp. 578-86, 2015

Tesfay, Clausen, Kim, Hegde, Wang, Miller, Deng, Blanchette, Arvedson, Miranti, Babitt, Lin, Peehl, Torti, Torti: "Hepcidin regulation in prostate and its disruption in prostate cancer." in: Cancer research, Vol. 75, Issue 11, pp. 2254-63, 2015 (Sample species: Human). Further details: Western Blotting

Scheers, Sandberg: "Iron transport through ferroportin is induced by intracellular ascorbate and involves IRP2 and HIF2α." in: Nutrients, Vol. 6, Issue 1, pp. 249-60, 2014 (Sample species: Human). Further details: Western Blotting

Kim, Jeong, Lee, Kim, Park, Kim, Koh, Shin, Jung, Kim, Lee, Oh, Kim, Park, Jeong, Lee, Park, Min, Jung, Choi, Choy, Choi: "Inverse agonist of estrogen-related receptor γ controls Salmonella typhimurium infection by modulating host iron homeostasis." in: Nature medicine, Vol. 20, Issue 4, pp. 419-24, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting

Scheers, Almgren, Sandberg: "Proposing a Caco-2/HepG2 cell model for in vitro iron absorption studies." in: The Journal of nutritional biochemistry, Vol. 25, Issue 7, pp. 710-5, 2014 (Sample species: Human). Further details: Western Blotting

Vercellotti, Khan, Nguyen, Chen, Bruzzone, Bechtel, Brown, Nath, Steer, Hebbel, Belcher: "H-ferritin ferroxidase induces cytoprotective pathways and inhibits microvascular stasis in transgenic sickle mice." in: Frontiers in pharmacology, Vol. 5, pp. 79, 2014 (Sample species: Mouse (Murine)). Further details: Western Blotting

Theurl, Nairz, Schroll, Sonnweber, Asshoff, Haschka, Seifert, Willenbacher, Wilflingseder, Posch, Murphy, Witcher, Theurl, Weiss: "Hepcidin as a predictive factor and therapeutic target in erythropoiesis-stimulating agent treatment for anemia of chronic disease in rats." in: Haematologica, Vol. 99, Issue 9, pp. 1516-24, 2014 (Sample species: Rat (Rattus)). Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

Choi, Koh, Lee, Kim, Song: "Effects of excess dietary iron and fat on glucose and lipid metabolism." in: The Journal of nutritional biochemistry, Vol. 24, Issue 9, pp. 1634-44, 2013 (Sample species: Mouse (Murine)). Further details: Western Blotting

Images

Product details Target details Application Details Handling References back to top
Supplier Images
Western Blotting (WB) image for anti-Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1) (AA 250-300), (Internal Region) antibody (ABIN439384) anti-Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1) (AA 250-300), (Internal Region) antibody
Flow Cytometry (FACS) image for anti-Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1) (AA 250-300), (Internal Region) antibody (ABIN439384) Flow Cytometry: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Histogram of Ferroportin ...
Flow Cytometry (FACS) image for anti-Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1) (AA 250-300), (Internal Region) antibody (ABIN439384) Flow Cytometry: Ferroportin/SLC40A1 Antibody [NBP1-21502] - Analysis using the PE con...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1) (AA 250-300), (Internal Region) antibody (ABIN439384) Immunohistochemistry-Paraffin: Ferroportin/SLC40A1 Antibody [NBP1-21502] - IHC-P dete...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1) (AA 250-300), (Internal Region) antibody (ABIN439384) Immunohistochemistry-Paraffin: Ferroportin/SLC40A1 Antibody [NBP1-21502] - IHC-P dete...
Immunofluorescence (IF) image for anti-Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1) (AA 250-300), (Internal Region) antibody (ABIN439384) Immunocytochemistry/Immunofluorescence: Ferroportin/SLC40A1 Antibody [NBP1-21502] - S...
Immunofluorescence (IF) image for anti-Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1) (AA 250-300), (Internal Region) antibody (ABIN439384) Immunocytochemistry/Immunofluorescence: Ferroportin/SLC40A1 Antibody was used as a c...