anti-Hamster FZD4 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended FZD4 Antibody (supplied by: Log in to see )

Antigen
Frizzled Family Receptor 4 (FZD4) Antibodies
  • CG4626
  • DFz4
  • Dfz4
  • Dm Fz4
  • Dmel\\CG4626
  • Fz4
  • anon-WO0170980.10
  • anon-WO0170980.11
  • fz1
  • zg01
  • CD344
  • EVR1
  • FEVR
  • FZD4S
  • Fz-4
  • FzE4
  • GPCR
  • hFz4
  • frizzled4
  • fz4
  • FZ-4
  • frizzled 4
  • frizzled class receptor 4
  • frizzled-4
  • frizzled class receptor 4 S homeolog
  • fz4
  • fzd4
  • Tsp_10376
  • FZD4
  • Fzd4
  • fzd4.S
Epitope
C-Term
36
25
16
15
11
8
8
4
4
4
4
3
3
3
3
2
2
1
1
1
1
1
Reactivity
Bat, Chicken, Cow (Bovine), Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
138
110
58
7
6
6
6
5
4
4
4
3
3
2
2
1
Host
Rabbit
131
27
15
8
Clonality
Polyclonal
Conjugate
This FZD4 antibody is un-conjugated
11
8
7
7
6
5
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
119
68
20
18
17
13
13
6
5
2
1
1
1
1
Supplier
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Catalog No. ABIN1048624
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
7 ABIN1048623 IHC IHC (p) Rabbit N-Term Log in to see Polyclonal 0

General

Antigen Frizzled Family Receptor 4 (FZD4) Antibodies
Epitope C-Term
(36), (25), (16), (15), (11), (8), (8), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Reactivity Bat, Chicken, Cow (Bovine), Dog (Canine), Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
(138), (110), (58), (7), (6), (6), (6), (5), (4), (4), (4), (3), (3), (2), (2), (1)
Host Rabbit
(131), (27), (15), (8)
Clonality
Polyclonal
Conjugate This FZD4 antibody is un-conjugated
(11), (8), (7), (7), (6), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(119), (68), (20), (18), (17), (13), (13), (6), (5), (2), (1), (1), (1), (1)
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Product Details anti-FZD4 Antibody

Target Details FZD4 Application Details Handling Images
Brand IHC-plus™
Specificity Human FZD4 / Frizzled 4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%) Lizard, Xenopus (92%).
Purification Immunoaffinity purified
Immunogen Synthetic 13 amino acid peptide from C-terminus of human FZD4 / Frizzled 4. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus (100%), Lizard, Xenopus (92%).

Type of Immunogen: Synthetic peptide
Plasmids, Primers & others

Target Details FZD4

Product Details anti-FZD4 Antibody Application Details Handling Images back to top
Antigen
Alternative Name FZD4 / Frizzled 4 (FZD4 Antibody Abstract)
Background Name/Gene ID: FZD4
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD4, CD344, CD344 antigen, EVR1, Frizzled family receptor 4, Frizzled-4, Fz-4, Fz4, HFz4, Exudative vitreoretinopathy 1, FEVR, FZD4S, FzE4, Frizzled 4, Frizzled homolog 4, WNT receptor frizzled-4
Gene ID 8322
Pathways WNT Signaling, Hormone Transport, Sensory Perception of Sound

Application Details

Product Details anti-FZD4 Antibody Target Details FZD4 Handling Images back to top
Application Notes Approved: IHC, IHC-P (25 μg/mL)
Comment

Target Species of Antibody: Human

Assay Procedure The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip
Restrictions For Research Use only

Handling

Product Details anti-FZD4 Antibody Target Details FZD4 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months

Images

Product Details anti-FZD4 Antibody Target Details FZD4 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Frizzled Family Receptor 4 (FZD4) (C-Term) antibody (ABIN1048624) Anti-FZD4 / Frizzled 4 antibody ABIN1048624 IHC staining of human kidney. Immunohist...
Immunohistochemistry (IHC) image for anti-Frizzled Family Receptor 4 (FZD4) (C-Term) antibody (ABIN1048624) Anti-FZD4 / Frizzled 4 antibody IHC of human Skin, Melanoma. Immunohistochemistry of ...