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|Antigen||Low Density Lipoprotein Receptor-Related Protein 6 (LRP6) Antibodies|
|Epitope||Internal Region Alternatives|
|Conjugate||This LRP6 antibody is un-conjugated Alternatives|
|Method Type||Peptide ELISA|
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
|2 references available|
|Supplier||Log in to see|
Product Details anti-LRP6 AntibodyTarget Details LRP6 Application Details Handling References for anti-LRP6 antibody (ABIN185307) Images
|Blocking Peptide||Blocking peptide for this product available: ABIN369437|
|Specificity||This antibody is expected to recognise an epitope corresponding to aa 1546-1560 of human LRP6 protein.|
|Cross-Reactivity||Mouse (Murine), Rat (Rattus), Dog (Canine)|
|Purification||Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.|
|Immunogen||Peptide with sequence CMTSVATAKGYTSDL, from the internal region of the protein sequence according to NP_002327.2.|
Target Details LRP6Product Details anti-LRP6 Antibody Application Details Handling References for anti-LRP6 antibody (ABIN185307) Images back to top
|Alternative Name||LRP6 (LRP6 Antibody Abstract)|
|Background||Synonyms: LRP6, low density lipoprotein receptor-related protein 6, low density lipoprotein-related protein 6|
|Gene ID||4040, 16974|
|Pathways||WNT Signaling, Tube Formation|
Application DetailsProduct Details anti-LRP6 Antibody Target Details LRP6 Handling References for anti-LRP6 antibody (ABIN185307) Images back to top
ELISA: antibody detection limit dilution 1:64000.
Western blot: Preliminary experiments gave bands at approx 50 kDa and 35 kDa in Human Adipose lysates after 0.1 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 180 kDa according to NP_002327.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
IHC: In paraffin embedded Human Kidney shows luminar membrane staining in DCT. Recommended concentration: 4-6 µg/mL.
|Restrictions||For Research Use only|
HandlingProduct Details anti-LRP6 Antibody Target Details LRP6 Application Details References for anti-LRP6 antibody (ABIN185307) Images back to top
|Buffer||Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Minimize freezing and thawing.|
|Storage Comment||Aliquot and store at -20 °C.|
References for anti-LRP6 antibody (ABIN185307)Product Details anti-LRP6 Antibody Target Details LRP6 Application Details Handling Images back to top
Giles, van Es, Clevers: "Caught up in a Wnt storm: Wnt signaling in cancer." in: Biochimica et biophysica acta, Vol. 1653, Issue 1, pp. 1-24, 2003
Tamai, Semenov, Kato, Spokony, Liu, Katsuyama, Hess, Saint-Jeannet, He: "LDL-receptor-related proteins in Wnt signal transduction." in: Nature, Vol. 407, Issue 6803, pp. 530-5, 2000
ImagesProduct Details anti-LRP6 Antibody Target Details LRP6 Application Details Handling References for anti-LRP6 antibody (ABIN185307) back to top