anti-Pig (Porcine) Rhodopsin antibody for Immunohistochemistry (Paraffin-embedded Sections)
Recommended Rhodopsin Antibody (supplied by: Log in to see )
- Antigen
-
Rhodopsin (RHO) Antibodies
show synonyms for this antigen- CSNBAD1
- OPN2
- RP4
- RH1
- Rh
- fi06d11
- wu:fi06d11
- zfo2
- zfrho
- Noerg1
- Opn2
- Ops
- opn2
- rh1
- rp4
- xrho
- PIGRHO1
- ops
- RDP1
- RHO
- rhodopsin
- rhodopsin L homeolog
- RHO
- Rho
- rho
- rho.L
- Epitope
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Extracellular Domain
Alternatives - Reactivity
-
Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus)
Alternatives - Host
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Rabbit
Alternatives - Clonality
- Conjugate
-
This Rhodopsin antibody is un-conjugated
Alternatives - Application
-
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Alternatives - Supplier
- Log in to see
- Supplier Product No.
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Catalog No. ABIN1049292
$ 484.00
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| Relevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | ABIN1960117 | ICC IF IHC IHC (p) WB | Mouse | IgG1, kappa | Log in to see | B630 | 0 | |||
| 1 | ABIN1960118 | IF IHC IHC (p) WB | Mouse | IgG1, kappa | Log in to see | A531 | 0 | |||
| 1 | ABIN2964046 | ICC IF IHC (p) WB | Mouse | IgG1 kappa | Log in to see | B630 | 0 |
General |
|
|---|---|
| Antigen | Rhodopsin (RHO) Antibodies |
| Epitope | Extracellular Domain Alternatives |
| Reactivity | Human, Monkey, Mouse (Murine), Pig (Porcine), Rabbit, Rat (Rattus) Alternatives |
| Host | Rabbit Alternatives |
| Clonality | |
| Conjugate | This Rhodopsin antibody is un-conjugated Alternatives |
| Application |
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Alternatives
|
| Supplier | Log in to see |
Product Details anti-Rhodopsin AntibodyTarget Details Rhodopsin Application Details Handling Images |
|
| Brand | IHC-plus™ |
| Specificity | Human RHO / Rhodopsin. BLAST analysis of the peptide immunogen showed no homology with other human proteins. |
| Predicted Reactivity | Percent identity with other species by BLAST analysis: Human, Monkey, Mouse, Rat, Rabbit, Pig, Cat, Elephant, Zebra finch (100%). |
| Purification | Immunoaffinity purified |
| Immunogen |
Synthetic 19 amino acid peptide from 2nd extracellular domain of human RHO / Rhodopsin. Percent identity with other species by BLAST analysis: Human, Monkey, Mouse, Rat, Rabbit, Pig, Cat, Elephant, Zebra finch (100%). Type of Immunogen: Synthetic peptide |
| Plasmids, Primers & others | |
Target Details RhodopsinProduct Details anti-Rhodopsin Antibody Application Details Handling Images back to top |
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| Antigen | |
| Alternative Name | Rhodopsin / rho (RHO Antibody Abstract) |
| Target Type | Chemical |
| Background |
Name/Gene ID: RHO Subfamily: Opsin Family: GPCR Synonyms: RHO, CSNBAD1, OPN2, Rhodopsin, RP4, Opsin 2, rod pigment, Opsin-2 |
| Gene ID | 6010 |
| Pathways | WNT Signaling, Sensory Perception of Sound, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction |
Application DetailsProduct Details anti-Rhodopsin Antibody Target Details Rhodopsin Handling Images back to top |
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| Application Notes | Approved: IHC, IHC-P (1 - 3 μg/mL) |
| Comment |
Target Species of Antibody: Human |
| Assay Procedure |
The IHC-pro Immunohistochemistry Protocol Tissue Preparation Formalin fixation and embedding in paraffin wax Tissue Sectioning Make 4-µm sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-drying oven for 45 minutes at 60°C Deparaffinization Wash slides in 3 changes of xylene – 5 minutes each at room temperature. Rehydration Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature. Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature. Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature. Rinse slides in gentle running distilled water – 5 minutes at room temperature. Antigen retrieval Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes Remove from heat and let stand at room temperature in buffer - 20 minutes Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature. Immunostaining Do not allow tissues to dry at any time during the staining procedure. Apply a universal protein block – 20 minutes at room temperature. Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature. Rinse slides in 1X TBST - 1 minute at room temperature. Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature. Rinse slides 1X TBST – 1 minute at room temperature. Apply alkaline phosphatase streptavidin – 30 minutes at room temperature. Rinse slides in 1X TBST - 1 minute at room temperature. Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature. Wash slides in distilled water – 1 minute at room temperature. Dehydrate This method should only be used if the chromogen substrate is alcohol insoluble. Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature. Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature. Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature. Wash slides in 3 changes of xylene – 1 minute each at room temperature. Apply coverslip |
| Restrictions | For Research Use only |
HandlingProduct Details anti-Rhodopsin Antibody Target Details Rhodopsin Application Details Images back to top |
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| Format | Liquid |
| Concentration | Lot specific |
| Buffer | PBS, less than 0.1 % sodium azide. |
| Preservative | Sodium azide |
| Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Storage | 4 °C,-20 °C |
| Storage Comment | Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles. |
| Expiry Date | 12 months |
ImagesProduct Details anti-Rhodopsin Antibody Target Details Rhodopsin Application Details Handling back to top |
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| Supplier Images |
Anti-RHO / Rhodopsin antibody ABIN1049292 IHC staining of human eye, retina. Immunoh...
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