Integrin beta 2 antibody (PE)

Catalog No. ABIN180773

Product Details anti-Integrin beta 2 Antibody (PE)

Target: Integrin beta 2 (ITGB2)

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Integrin beta 2 antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Specificity: This antibody recognises the Porcine homologue of Human CD18. Clone PNK-I immunoprecipitates molecules of 166, 155 and 95 kDa under reducing and non-reducing conditions. Clone PNK-I has been reported to inhibit Porcine NK activity without affecting antibody-dependent cellular cytoxicity.

Characteristics: Synonyms: Integrin beta-2, MFI7, Cell surface adhesion glycoproteins LFA-1/CR3/p150, 95 subunitbeta, Complement receptor C3 subunit beta

Purification: Affinity chromatography on Protein G

Immunogen: Porcine large granular lymphocytes. Spleen cells from immunised Balb/c mice were fusedwith cells of the mouse P3-X63-Ag8. 653 myeloma cell line.

Clone: PNK-I

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: use 10 μL of neat antibody to label 10^6 cells in 10 l.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL distilled water.

Buffer: PBS, pH 7.4 containing 0.09 % Sodium Azide and 1 % BSA and 5 % Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at +4 °C. This product should be stored undiluted. DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Integrin beta 2

Target: Integrin beta 2 (ITGB2)

Alternative Name: CD18 / ITGB2 (ITGB2 Products)

Synonyms: CD18 antibody, LAD antibody, LCAMB antibody, LFA-1 antibody, MAC-1 antibody, MF17 antibody, MFI7 antibody, 2E6 antibody, AI528527 antibody, Cd18 antibody, Lfa1 antibody, ITGB2 antibody, itgb2 antibody, itgb2-a antibody, itgb2-b antibody, integrin subunit beta 2 antibody, integrin beta 2 antibody, integrin subunit beta 2 S homeolog antibody, ITGB2 antibody, Itgb2 antibody, itgb2.S antibody

Background: CD18 is an integrin. Integrins are integral cell surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, beta 2 combines with the alpha L chain to form the integrin LFA1, and combines with the alpha M chain to form the integrin Mac1. Integrins are known to participate in cell adhesion as well as cell surface mediated signalling. CD18 is expressed by most leucocytes. Defects in the CD18 gene result in leukocyte adhesion deficiency.Synonyms: 95 subunit beta, Cell surface adhesion glycoproteins LFA-1/CR3/p150, Complement receptor C3 subunit beta, Integrin beta-2, MFI7

Gene ID: 396943

UniProt: P53714

Pathways: NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation