This Integrin beta 2 antibody is conjugated to Biotin
Application
Flow Cytometry (FACS)
Brand
BD Pharmingen™
Characteristics
The C71/16 antibody reacts with the common β2 chain of LFA-1 (CD11a/CD18, αLβ2 integrin), Mac-1 (CD11b/CD18, αMβ2 integrin), and gp150, 95 (CD11c/CD18, αxβ2 integrin). Expression of CD18 is limited to leukocytes, where it is widely distributed in consort with the three integrin α chains (CD11a, CD11b, and CD11c). Among splenocytes, NK cells have the highest density of CD18, and T lymphocytes express a higher density than the remaining cells. The β2 integrins are important mediators of leukocyte-endothelium interactions.
BD Pharmingen™ Biotin Rat Anti-Mouse CD18 - Biotin - Clone C71/16 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg
Purification
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen
Cell membrane glycoproteins from mouse T-cell lymphoma BW5147
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
Storage
4 °C
Storage Comment
Store undiluted at 4°C.
Springer: "Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm." in: Cell, Vol. 76, Issue 2, pp. 301-14, (1994) (PubMed).
Springer: "Adhesion receptors of the immune system." in: Nature, Vol. 346, Issue 6283, pp. 425-34, (1990) (PubMed).
Trowbridge, Omary: "Molecular complexity of leukocyte surface glycoproteins related to the macrophage differentiation antigen Mac-1." in: The Journal of experimental medicine, Vol. 154, Issue 5, pp. 1517-24, (1982) (PubMed).