Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (Middle Region) antibody

Details for Product No. ABIN2782429, Supplier: Log in to see
Antigen
  • p40phox
  • AI451400
  • NCF
  • P40PHOX
  • SH3PXD4
  • p40phox protein
  • neutrophil cytosolic factor 4
  • neutrophil cytosolic factor 4, 40kDa
  • p40phox
  • LOC100136241
  • Ncf4
  • NCF4
Epitope
Middle Region
19
9
9
4
4
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
117
57
43
6
6
4
4
3
3
2
Host
Rabbit
90
20
11
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Western Blotting (WB)
74
41
33
26
23
5
4
3
3
2
2
1
Options
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Immunogen The immunogen is a synthetic peptide directed towards the middle region of human NCF4
Blocking Peptide Blocking peptide for this product available: ABIN980523
Sequence TGIFPLSFVK ILKDFPEEDD PTNWLRCYYY EDTISTIKSV AWEGGACPAF
Predicted Reactivity Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 79%, Rabbit: 93%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against NCF4. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Alternative Name NCF4 (NCF4 Antibody Abstract)
Background NCF4 is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed. The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Alias Symbols: MGC3810, NCF, P40PHOX, SH3PXD4
Protein Interaction Partner: NCF2, UBC, CORO1A, MSN, CYBB, NCF1, CYBA, TXN, PRKCD, XRCC6, PRKDC,
Protein Size: 348
Molecular Weight 39 kDa
Gene ID 4689
NCBI Accession NM_013416, NP_038202
UniProt A8K4F9
Research Area Metabolism
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 348 AA

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Supplier Images
Western Blotting (WB) image for anti-Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (Middle Region) antibody (ABIN2782429) WB Suggested Anti-NCF4 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...
Background publications Chen, He, Minshall, Dinauer, Ye: "Characterization of a mutation in the Phox homology domain of the NADPH oxidase component p40phox identifies a mechanism for negative regulation of superoxide production." in: The Journal of biological chemistry, Vol. 282, Issue 41, pp. 30273-84, 2007 (PubMed).