c-MYC antibody (C-Term) (Alkaline Phosphatase (AP))

Catalog No. ABIN320077

This Mouse Monoclonal antibody specifically detects c-MYC in WB, IHC (p), IHC (fro) and EIA. It exhibits reactivity toward Human.

Quick Overview for c-MYC antibody (C-Term) (Alkaline Phosphatase (AP)) (ABIN320077)

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This c-MYC antibody is conjugated to Alkaline Phosphatase (AP)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: 9-00E-010

Product Details anti-c-MYC Antibody (Alkaline Phosphatase (AP))

Binding Specificity: C-Term

Sequence: EQKLISEEDL

Specificity: This antibody detects the 62 kDa c-myc gene product, which is involved in the regulation of the cell cycle and cell growth. This antibody may also be used to detect the commonly used c-Myc 'Tag'.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Immunogen: Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of Human c-myc. Spleen cells from immunised Balb/c mice were fused with cells of the myeloma cell line.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/500-1/5000. Western Blot: 1/100-1/500 (Non-Reducing Conditions). Detects a band of approximately 62 kDa in Colo 320HSR cell lysates. Immunohistochemistry on Frozen and Paraffin Sections: This product does not requireprotein digestion pre-treatment of paraffin sections prior to staining. It does not requireantigen retrieval using heat treatment prior to staining of paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Target Details for c-MYC

Target: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Alternative Name: C-Myc

Background: P62c-myc is primarily located to the cell nucleus, but has also been shown to localised to the cytoplasm in several cell lines. Overexpression of c-myc has been reported in a wide variety of human cancers.Synonyms: c-myc tag, myc tag, myc-tag

Gene ID: 4609

NCBI Accession: NP_002458

UniProt: P01106

Pathways: p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Warburg Effect