Anterior Pharynx Defective 1 Homolog A (C. Elegans) (APH1A) (N-Term), (AA 77-109) antibody

Details for Product No. ABIN390272
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Antigen
Epitope
N-Term, AA 77-109
(24), (7), (7), (5), (5), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(71), (40), (27), (13), (13), (13)
Host
Rabbit
(65), (6)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(60), (41), (15), (14), (10), (2), (1), (1)
Pubmed 5 references available
Quantity 400 µL
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Catalog No. ABIN390272
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Immunogen This APH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-109 AA from the N-terminal region of human APH1.
Clone RB4994
Isotype Ig
Specificity This APH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human APH1.
Predicted Reactivity Mouse (Murine)
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name APH1
Background APH1 is an essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid precursor protein). The gamma-secretase complex is minimally composed of a presenilin homodimer (PSEN1 or PSEN2), nicastrin (NCSTN), APH1 (APH1A or APH1B) and PEN2, although other components may exist. APH1 probably represents a stabilizing cofactor for the presenilin homodimer that promotes the formation of a stable complex.
Synonyms: Gamma-secretase subunit APH-1A, APH-1a, Aph-1alpha, Presenilin-stabilization factor, PSF
Molecular Weight 28996 DA
Gene ID 51107
UniProt Q96BI3
Research Area Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Anterior Pharynx Defective 1 Homolog A (C. Elegans) (APH1A) (N-Term), (AA 77-109) antibody Western blot analysis of anti-APH1 Antibody (N-term) (ABIN390272) in A2058 cell line lysates (35 µg/lane). APH1 (arrow) was detected using the purified polyclonal antibody.
anti-Anterior Pharynx Defective 1 Homolog A (C. Elegans) (APH1A) (N-Term), (AA 77-109) antibody (2) Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with APH1 antibody (N-term) (ABIN390272), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Background publications Edbauer, Winkler, Regula et al.: "Reconstitution of gamma-secretase activity." in: Nature cell biology, Vol. 5, Issue 5, pp. 486-8, 2003 (PubMed).

Kimberly, LaVoie, Ostaszewski et al.: "Gamma-secretase is a membrane protein complex comprised of presenilin, nicastrin, Aph-1, and Pen-2." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 11, pp. 6382-7, 2003 (PubMed).

Marlow, Canet, Haugabook et al.: "APH1, PEN2, and Nicastrin increase Abeta levels and gamma-secretase activity." in: Biochemical and biophysical research communications, Vol. 305, Issue 3, pp. 502-9, 2003 (PubMed).

Clark, Gurney, Abaya et al.: "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." in: Genome research, Vol. 13, Issue 10, pp. 2265-70, 2003 (PubMed).

Ota, Suzuki, Nishikawa et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, 2003 (PubMed).

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