Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (AA 260-289), (C-Term) antibody

Details for Product No. ABIN391905, Supplier: Log in to see
Antigen
  • p40phox
  • AI451400
  • NCF
  • P40PHOX
  • SH3PXD4
  • p40phox protein
  • neutrophil cytosolic factor 4
  • neutrophil cytosolic factor 4, 40kDa
  • p40phox
  • LOC100136241
  • Ncf4
  • NCF4
Epitope
AA 260-289, C-Term
19
9
8
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human
117
58
44
7
7
5
4
4
3
3
Host
Rabbit
90
20
11
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
74
41
33
26
22
4
4
3
3
2
2
1
Options
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Immunogen This NCF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 260-289 AA from the C-terminal region of human NCF4.
Clone RB18508
Isotype Ig
Specificity This NCF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 267~297 amino acids from the C-terminal region of human NCF4.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name NCF4 (NCF4 Antibody Abstract)
Background NCF4 is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2(NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity.
Synonyms: Neutrophil cytosol factor 4, NCF-4, Neutrophil NADPH oxidase factor 4, p40-phox, p40phox, SH3 and PX domain-containing protein 4, SH3PXD4, NCF4
Molecular Weight 39032 DA
Gene ID 4689
UniProt Q15080
Research Area Cell Signaling, Cancer, Metabolism, Cell Structure
Application Notes WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (AA 260-289), (C-Term) antibody (ABIN391905) Western blot analysis of NCF4 antibody (C-term) (ABIN391905) in K562 cell line lysate...
Immunohistochemistry (IHC) image for anti-Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (AA 260-289), (C-Term) antibody (ABIN391905) Formalin-fixed and paraffin-embedded human lymphoma reacted with NCF4 Antibody (C-ter...
Flow Cytometry (FACS) image for anti-Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (AA 260-289), (C-Term) antibody (ABIN391905) NCF4 Antibody (C-term) (ABIN391905) flow cytometry analysis of K562 cells (bottom his...
Background publications Glas, Seiderer, Pasciuto, Tillack, Diegelmann, Pfennig, Konrad, Schmechel, Wetzke, Török, Stallhofer, Jürgens, Griga, Klein, Epplen, Schiemann, Mussack, Lohse, Göke, Ochsenkühn, Folwaczny, Müller-Myhsok, Brand: "rs224136 on chromosome 10q21.1 and variants in PHOX2B, NCF4, and FAM92B are not major genetic risk factors for susceptibility to Crohn's disease in the German population." in: The American journal of gastroenterology, Vol. 104, Issue 3, pp. 665-72, 2009 (PubMed).

Leto, Adams, de Mendez: "Assembly of the phagocyte NADPH oxidase: binding of Src homology 3 domains to proline-rich targets." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 22, pp. 10650-4, 1994 (PubMed).

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