V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) (AA 379-408), (Thr400) antibody

Details for Product No. ABIN392248
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Antigen
Synonyms
B-Raf, braf1, rafb1, b-raf1, sierrabraf, BRAF, 9930012E13Rik, AA120551, AA387315, AA473386, B-raf, Braf-2, Braf2, C230098H17, C87398, D6Ertd631e, Braf, HYPT11, SME, Sm-E, B-RAF1, BRAF1, NS7, RAFB1, C- ... show more
B-Raf, braf1, rafb1, b-raf1, sierrabraf, BRAF, 9930012E13Rik, AA120551, AA387315, AA473386, B-raf, Braf-2, Braf2, C230098H17, C87398, D6Ertd631e, Braf, HYPT11, SME, Sm-E, B-RAF1, BRAF1, NS7, RAFB1, C-RMIL, RMIL show less
Epitope
AA 379-408, Thr400
(18), (17), (14), (13), (13), (13), (12), (10), (9), (6), (6), (6), (4), (4), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(252), (23), (20), (6), (4), (1), (1), (1)
Host
Rabbit
(168), (79), (5), (2)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(12), (12), (12), (9), (9), (9), (3), (3), (3), (3), (3), (3), (3), (3)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(176), (123), (82), (60), (50), (30), (29), (14), (10), (2), (1), (1)
Pubmed 3 references available
Quantity 400 μL
Options
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Catalog No. ABIN392248
291.50 $
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Immunogen This BRAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 379-408 AA from human BRAF.
Clone RB13721
Isotype Ig
Specificity This BRAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T400 of human BRAF.
Predicted Reactivity Mouse (Murine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name BRAF
Background BRAF, a member of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. This cytoplasmic protein is expressed in brain and testis. Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL). This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.
Synonyms: B-Raf proto-oncogene serine/threonine-protein kinase, p94, v-Raf murine sarcoma viral oncogene homolog B1
Molecular Weight 84490 DA
Gene ID 673
UniProt P15056
Research Area Apoptosis/Necrosis, Protein Modifications, Cell Structure, Cancer, Neurology
Application Notes WB = 1:1000, IHC = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) (AA 379-408), (Thr400) antibody Western blot analysis of BRAF (arrow) using rabbit polyclonal BRAF Antibody (T400) (ABIN392248). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the BRAF gene (Lane 2) (Origene Technologies).
anti-V-Raf Murine Sarcoma Viral Oncogene Homolog B1 (BRAF) (AA 379-408), (Thr400) antibody (2) Formalin-fixed and paraffin-embedded human brain tissue reacted with BRAF-pT400, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Background publications Davies, Bignell, Cox et al.: "Mutations of the BRAF gene in human cancer." in: Nature, Vol. 417, Issue 6892, pp. 949-54, 2002 (PubMed).

Naoki, Chen, Richards et al.: "Missense mutations of the BRAF gene in human lung adenocarcinoma." in: Cancer research, Vol. 62, Issue 23, pp. 7001-3, 2002 (PubMed).

Hingorani, Jacobetz, Robertson et al.: "Suppression of BRAF(V599E) in human melanoma abrogates transformation." in: Cancer research, Vol. 63, Issue 17, pp. 5198-202, 2003 (PubMed).

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