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BRAF antibody (V-Raf Murine Sarcoma Viral Oncogene Homolog B1) (AA 379-408)

Details for Product anti-BRAF Antibody No. ABIN392248, Supplier: Login to see
Antigen
  • B-Raf
  • braf1
  • rafb1
  • b-raf1
  • sierrabraf
  • BRAF
  • 9930012E13Rik
  • AA120551
  • AA387315
  • AA473386
  • B-raf
  • Braf-2
  • Braf2
  • C230098H17
  • C87398
  • D6Ertd631e
  • Braf
  • HYPT11
  • SME
  • Sm-E
  • B-RAF1
  • BRAF1
  • NS7
  • RAFB1
  • C-RMIL
  • RMIL
Alternatives
anti-Human BRAF antibody for Western Blotting
Epitope
AA 379-408, Thr400
29
24
23
22
21
17
17
17
16
14
14
13
9
8
7
6
5
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
393
51
43
6
4
1
1
1
Host
Rabbit
285
105
2
2
Clonality (Clone)
Polyclonal ()
Conjugate
This BRAF antibody is un-conjugated
20
20
20
17
17
17
3
3
3
3
3
3
3
3
3
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
265
213
163
83
56
49
33
20
12
4
2
1
1
1
Supplier
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Immunogen This BRAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 379-408 AA from human BRAF.
Clone RB13721
Isotype Ig
Specificity This BRAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T400 of human BRAF.
Predicted Reactivity Mouse (Murine)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name BRAF (BRAF Antibody Abstract)
Background BRAF, a member of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. This cytoplasmic protein is expressed in brain and testis. Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL). This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.
Synonyms: B-Raf proto-oncogene serine/threonine-protein kinase, p94, v-Raf murine sarcoma viral oncogene homolog B1
Molecular Weight 84490 DA
Gene ID 673
UniProt P15056
Research Area Apoptosis/Necrosis, Protein Modifications, Cell Structure, Cancer, Neurology
Pathways MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway
Application Notes WB = 1:1000, IHC (p) = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-BRAF antibody (V-Raf Murine Sarcoma Viral Oncogene Homolog B1) (AA 379-408) (ABIN392248) Western blot analysis of BRAF (arrow) using rabbit polyclonal BRAF Antibody (T400) (A...
Immunohistochemistry (IHC) image for anti-BRAF antibody (V-Raf Murine Sarcoma Viral Oncogene Homolog B1) (AA 379-408) (ABIN392248) Formalin-fixed and paraffin-embedded human brain tissue reacted with BRAF-pT400, whic...
Background publications Hingorani, Jacobetz, Robertson et al.: "Suppression of BRAF(V599E) in human melanoma abrogates transformation." in: Cancer research, Vol. 63, Issue 17, pp. 5198-202, 2003 (PubMed).

Naoki, Chen, Richards et al.: "Missense mutations of the BRAF gene in human lung adenocarcinoma." in: Cancer research, Vol. 62, Issue 23, pp. 7001-3, 2002 (PubMed).

Davies, Bignell, Cox et al.: "Mutations of the BRAF gene in human cancer. ..." in: Nature, Vol. 417, Issue 6892, pp. 949-54, 2002 (PubMed).